Bacterionanofibers Applicable to a New Method for Surface Immobilization of Microbial Cells

ABSTRACT We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.

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A new method for immobilization of microbial cells by cross-linking

Applied Biochemistry and Microbiology ◽

10.1007/bf02742586 ◽

2000 ◽

Vol 36 (3) ◽

pp. 310-314 ◽

Cited By ~ 1

Author(s):

V. A. Abelyan

Keyword(s):

New Method ◽

Cross Linking ◽

Microbial Cells

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Method to Rapidly Enumerate Salmonella on Chicken Carcasses

Journal of Food Protection ◽

10.4315/0362-028x-47.12.932 ◽

1984 ◽

Vol 47 (12) ◽

pp. 932-936 ◽

Cited By ~ 11

Author(s):

S. G. HAWA ◽

G. J. MORRISON ◽

G. H. FLEET

Keyword(s):

New Method ◽

Lysine Decarboxylase ◽

Detection Rates ◽

Microbial Cells ◽

Rapid Enumeration

A method for rapid enumeration of Salmonella on chicken carcasses was developed. Carcass rinses were centrifuged to sediment and concentrate Salmonella and other microbial cells. After washing and resuspending the pelleted cells to 1.0 ml, Salmonella was selectively isolated and differentiated from other species by plating onto newly developed dulcitol bile novobiocin agar. Rapid lysine decarboxylase and ONPG tests were developed for biochemical confirmation of presumptive Salmonella colonies. Fully confirmed Salmonella counts were obtained within 48 h. The new method gave Salmonella counts and detection rates that were significantly higher than those found by conventional enrichment, plating procedures.

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New Method of Immobilization of Microbial Cells by Capture on the Surface of Insoluble Pyridinium-type Resin

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1990.tb18276.x ◽

1990 ◽

Vol 613 (1 Enzyme Engine) ◽

pp. 856-857

Author(s):

NARIYOSHI KAWABATA ◽

SADAKI NISHIMURA ◽

TOHRU YOSHIMURA

Keyword(s):

New Method ◽

Microbial Cells

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Selective Sampling and Orientation of Non-Homogeneous Tissues

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100238 ◽

1968 ◽

Vol 26 ◽

pp. 98-99

Author(s):

C. C. Clawson ◽

L. W. Anderson ◽

R. A. Good

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Light Microscopy ◽

Random Sampling ◽

New Method ◽

Microscope Examination ◽

Small Areas ◽

Selective Sampling ◽

Large Numbers ◽

Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

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