scholarly journals Advanced glycation end-products reduce podocyte adhesion by activating the renin-angiotensin system and increasing integrin-linked kinase

2013 ◽  
Vol 6 (6) ◽  
pp. 1494-1498 ◽  
Author(s):  
CAILIAN CHENG ◽  
ZHENDA ZHENG ◽  
CHENGGANG SHI ◽  
XUN LIU ◽  
ZENGCHUN YE ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Renin Angiotensin System ◽  
Advanced Glycation ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Glycation End Products ◽  
End Products ◽  
Integrin Linked Kinase

scholarly journals Blockade of Renin-Angiotensin System Attenuates Advanced Glycation End Products-Mediated Signaling Pathways

2010 ◽  
Vol 17 (6) ◽  
pp. 590-600 ◽  
Author(s):  
Masashi Kamioka ◽  
Toshiyuki Ishibashi ◽  
Koichi Sugimoto ◽  
Hironori Uekita ◽  
Ryoji Nagai ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Advanced Glycation End Products ◽  
Renin Angiotensin System ◽  
Advanced Glycation ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Glycation End Products ◽  
End Products

scholarly journals Advanced glycation end-products activate the renin-angiotensin system through the RAGE/PI3-K signaling pathway in podocytes

2012 ◽  
Vol 35 (5) ◽  
pp. 282 ◽  
Author(s):  
Cailan L. Cheng ◽  
Ying Tang ◽  
Zhenda Zheng ◽  
Xun Liu ◽  
Zengchun C. Ye ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Western Blot ◽  
Advanced Glycation End Products ◽  
Renin Angiotensin System ◽  
Ang Ii ◽  
Advanced Glycation ◽  
Angiotensin System ◽  
Glycation End Products ◽  
End Products ◽  
Ace Activity

Purpose: The purpose of this study was to investigate the effects of advanced glycation end-products (AGEs) on the components of the renin-angiotensin system (RAS) in podocytes and to understand the mechanism of these effects. Methods: Immortalized mouse podocytes were exposed to various concentrations of AGEs for different time intervals. The expression levels of angiotensinogen (AGT), angiotensin II type 1 and 2 receptors (AT1R and AT2R) and renin were examined by real-time PCR and western blot; the receptor for AGEs (RAGE) and both Akt and phosphorylated Akt were examined by western blot; levels of angiotensin II (Ang II) were assayed by ELISA, and the activity of angiotensin-converting enzyme (ACE) was evaluated by measuring the production of hippuric acid in vitro. Results: Treatment with AGEs resulted in significant increases in the expression of AGT (62%, P=0.002) and AT1R (59%, P=0.01). Moreover, Ang II levels increased significantly in both cell lysates (70%, P=0.018) and conditioned media (65%, P=0.01). ACE activity was also significantly higher in cell lysates (68% , P= 0.035) and conditioned media (65%, P=0.023). There were no changes in renin or AT2R expression (P > 0.05). AGEs did increase the expression of RAGE by 50% (P=0.012) and the phosphorylation of Akt by 100% (P=0.001). When podocytes were pretreated with anti-RAGE antibody (50 µg/ml) or the phosphoinositide 3-kinase (PI3-K) inhibitor, LY294002 (10 µM), the AGEs-induced increases in AGT and AT1R expression were reduced. Likewise, Ang II levels and ACE activity decreased significantly. Conclusion: AGEs activate the RAS in podocytes through the RAGE-PI3-K/Akt-dependent pathway and lead to an increase in podocyte apoptosis.

scholarly journals Hyperactivated RAGE in Comorbidities as a Risk Factor for Severe COVID-19—The Role of RAGE-RAS Crosstalk

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 876
Author(s):  
Sara Chiappalupi ◽  
Laura Salvadori ◽  
Rosario Donato ◽  
Francesca Riuzzi ◽  
Guglielmo Sorci
Keyword(s):  
Gene Polymorphisms ◽  
Renin Angiotensin System ◽  
Molecular Target ◽  
Advanced Glycation ◽  
Angiotensin System ◽  
Major Player ◽  
Glycation End Products ◽  
End Products ◽  
And Oxidative Stress

The receptor for advanced glycation-end products (RAGE) is a multiligand receptor with a role in inflammatory and pulmonary pathologies. Hyperactivation of RAGE by its ligands has been reported to sustain inflammation and oxidative stress in common comorbidities of severe COVID-19. RAGE is essential to the deleterious effects of the renin–angiotensin system (RAS), which participates in infection and multiorgan injury in COVID-19 patients. Thus, RAGE might be a major player in severe COVID-19, and appears to be a useful therapeutic molecular target in infections by SARS-CoV-2. The role of RAGE gene polymorphisms in predisposing patients to severe COVID-19 is discussed. 

scholarly journals N-Acetyl Cysteine Attenuated the Deleterious Effects of Advanced Glycation End-Products on the Kidney of Non-Diabetic Rats

2016 ◽  
Vol 40 (3-4) ◽  
pp. 608-620 ◽  
Author(s):  
Karina Thieme ◽  
Karolline S. Da Silva ◽  
Nelly T. Fabre ◽  
Sergio Catanozi ◽  
Maria Beatriz Monteiro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Advanced Glycation End Products ◽  
Kidney Diseases ◽  
Renin Angiotensin System ◽  
Macrophage Infiltration ◽  
Concomitant Treatment ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Acetyl Cysteine

Aim: To assess the renal effects of chronic exposure to advanced glycation end-products (AGEs) in the absence of diabetes and the potential impact of concomitant treatment with the antioxidant N-acetyl cysteine (NAC). Methods: Wistar rats received intraperitoneally 20 mg/kg/day of albumin modified (AlbAGE) or not (AlbC) by advanced glycation for 12 weeks and oral NAC (600mg/L; AlbAGE+NAC and AlbC+NAC, respectively). Biochemical, urinary and renal morphological analyses; carboxymethyl-lysine (CML, an AGE), CD68 (macrophage infiltration), and 4-hydroxynonenal (4-HNE, marker of oxidative stress) immunostaining; intrarenal mRNA expression of genes belonging to pathways related to AGEs (Ager, Ddost, Nfkb1), renin-angiotensin system (Agt, Ren, Ace), fibrosis (Tgfb1, Col4a1), oxidative stress (Nox4, Txnip), and apoptosis (Bax, Bcl2); and reactive oxidative species (ROS) content were performed. Results: AlbAGE significantly increased urine protein-to-creatinine ratio; glomerular area; renal CML content and macrophage infiltration; expression of Ager, Nfkb1, Agt, Ren, Tgfb1, Col4a1, Txnip, Bax/Bcl2 ratio; and 4-HNE and ROS contents. Some of these effects were attenuated by NAC concomitant treatment. Conclusion: Because AGEs are highly consumed in modern diets and implicated in the progression of different kidney diseases, NAC could be a therapeutic intervention to decrease renal damage, considering that long-term restriction of dietary AGEs is difficult to achieve in practice.

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