PROGNOSTIC VALUE OF PERIODIC ACID-SCHIFF (PAS) STAINING OF FINE-NEEDLE ASPIRATES FROM PATIENTS WITH PRIMARY HEPATOCELLULAR-CARCINOMA

The use of eyewash solutions in Japan, especially in patients with allergic conjunctivitis and contact lens wearers, has been increasing. Our aim was to investigate how the use of preservative-free eyewash solution in healthy eyes for one month affects corneal safety and ocular surface mucin. We analyzed 42 eyes of 21 individuals (17 males, four females; mean age: 36.1 ± 7.4 years) without ocular allergies, dry eyes, or other ocular diseases through a prospective study. Eyes were randomized to a wash group (group one) and a nonwash follow up group (group two). We evaluated the dry eye-related quality-of-life score (DEQS), tear film breakup time (TBUT), fluorescein staining score, mRNA expression of MUC5AC and MUC16, MUC16 immunohistochemistry, and MUC5AC periodic acid Schiff (PAS) staining. There was a significant decrease in DEQS scores after one month of eyewash use (p < 0.05). There were no significant differences in other evaluation items that were analyzed (all p > 0.05). Furthermore, no significant differences were observed between group one and group two in all endpoints (all p > 0.05). The results suggest that one month use of a nonpreserved eyewash solution has no detrimental effects on the tear film and the ocular surface mucins.

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Prognostic Value of Pre-Operative Platelet to Lymphocyte Ratio in Patients with Resected Primary Hepatocellular Carcinoma

Clinical Laboratory ◽

10.7754/clin.lab.2016.160414 ◽

2016 ◽

Vol 62 (11/2016) ◽

Cited By ~ 4

Author(s):

Lihong Dong ◽

Kai Bai ◽

Yi Cao ◽

Qiaojia Huang ◽

Lizhi Lv ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Prognostic Value ◽

Primary Hepatocellular Carcinoma ◽

Platelet To Lymphocyte Ratio ◽

Lymphocyte Ratio

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Production of capsular material by equine trophoblast transplanted into immunodeficient mice

Reproduction ◽

10.1530/rep.0.1250855 ◽

2003 ◽

pp. 855-863 ◽

Cited By ~ 21

Author(s):

A Albihn ◽

RO Waelchli ◽

J Samper ◽

JG Oriol ◽

BA Croy ◽

...

Keyword(s):

Periodic Acid ◽

Endometrial Biopsy ◽

Specific Staining ◽

Periodic Acid Schiff ◽

In Vitro Techniques ◽

Immunodeficient Mice ◽

Xenogeneic Transplantation ◽

Pas Staining ◽

Capsular Material

A novel xenogeneic transplantation approach was used to determine whether it is embryonic or maternal tissue that produces the material that gives rise to the mucin-like glycoprotein of the equine embryonic capsule. Endometrial biopsy samples and conceptuses from six mares at days 13-15 after ovulation were prepared as 1 mm(3) grafts of endometrium, trophoblast and capsule for transplantation, alone or in combination, into various sites in 88 immunodeficient (severe combined immunodeficient or RAG2/gamma(c) double mutant) mice. The overall recovery rate of grafts was over 50%, reaching 100% with experience and use of the renal subcapsular space exclusively. Periodic acid-Schiff (PAS) staining demonstrated capsule-like extracellular glycoprotein secretions at the graft site in 11 of 22 sites examined. Strong PAS-positive reactions (5-7 microm thick) were found in four of six sites containing trophoblast alone, five of six endometrium plus trophoblast sites, and zero of eight grafts of endometrium alone. Two recovered grafts of capsule were also PAS-positive. The secreted glycoprotein was identified as equine embryonic capsule material by using a monoclonal antibody (mAb) specific to equine capsule (mAb OC-1) in two experiments. In the first, in cryosections, this antibody bound to 19 of 19 recovered trophoblast graft secretions (including those in 12 from mice that had not received endometrium at any site), ten of ten recovered endometrium plus trophoblast grafts, and zero of 12 recovered endometrial grafts from mice in which trophoblast had been grafted to the same site or another site in the same mouse. In the second experiment, in paraformaldehyde-fixed sections of grafts from 11 mice, specific staining, identical to that shown by grafted capsule, was obtained with grafts of trophoblast (both alone and in combination with endometrium) but not with grafts of endometrium. These results support the contention that trophoblast is the principal source of equine embryonic capsule. In addition, they demonstrate that xenogeneic grafting is a useful means of culturing endometrium and conceptus tissues outside the mare when in vitro techniques do not suffice.

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Isolation, culture and characterization of chicken primordial germ cells

Chinese Journal of Agricultural Biotechnology ◽

10.1079/cjb2006107 ◽

2006 ◽

Vol 3 (3) ◽

pp. 183-188 ◽

Cited By ~ 3

Author(s):

Tang Xin-Yan ◽

Zeng Wei-Dong ◽

Mi Yu-Ling ◽

Liu Hong-Yun ◽

Zhang Cai-Qiao

Keyword(s):

Germ Cells ◽

Gradient Centrifugation ◽

Periodic Acid ◽

Primordial Germ Cells ◽

Culture Conditions ◽

Nuclear Antigen ◽

Periodic Acid Schiff ◽

Ficoll Density Gradient Centrifugation ◽

Pas Staining ◽

Proliferating Activity

AbstractPrimordial germ cells (PGCs) were isolated from the genital ridges of chicken (Gallus domesticus) embryos at the 19th stage and purified by Ficoll density-gradient centrifugation. PGCs were co-cultured with somatic cells in preliminary culture and subcultured. Identification of PGCs was carried out by histochemical methods, including alkaline phosphatase (AKP) and periodic acid–Schiff (PAS). The proliferating activity of PGCs in subculture was demonstrated by immunocytochemistry of proliferating cell nuclear antigen (PCNA). Meanwhile, proliferating PGCs were compared under different culture conditions of 5–20% fetal cattle serum (FCS), insulin–transferrin–selenite (ITS) medium, conditioned medium (CM), 15% FCS+ITS, 15% FCS+40% CM. The results showed that the cultured PGCs were positive for AKP and PAS staining and displayed intensive proliferating activity by PCNA. The PGCs without centrifugation grew better than those with centrifugation. The PGCs formed larger colonies in media with 5% FCS or ITS than other media, indicating that 5% FCS or ITS supplemented media could be an ideal culture system for PGC proliferation in the PGC-somatic cell co-culture, in addition to the embryonic fibroblast feeder layer.

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The role of histopathology in the diagnosis of dermatophytoses / Značaj patohistološkog nalaza u dijagnostici dermatofitoza

Serbian Journal of Dermatology and Venerology ◽

10.2478/v10249-011-0021-0 ◽

2010 ◽

Vol 2 (2) ◽

pp. 45-53 ◽

Cited By ~ 3

Author(s):

Đorđi Gocev ◽

Katerina Damevska

Keyword(s):

Fungal Infection ◽

Periodic Acid ◽

False Negative ◽

Histopathological Analysis ◽

Unexpected Finding ◽

Skin Infections ◽

Specific Staining ◽

Periodic Acid Schiff ◽

Skin Reactions ◽

Pas Staining

Abstract Histopathological analysis is not a routine procedure for diagnosing fungal skin infections. In the histopathological specimens, fungi are visible only when using special stain such as periodic acid-Schiff (PAS). However, histopathological analysis may not be performed in small laboratories. Histopathological characteristics of fungal skin infections are not specific. In all skin biopsy cases, obtained without clinical suspicion of fungal infection, the knowledge of certain, most frequent histopathological reaction patterns, as well as specific histopathological indicators (a diagnostic histopathological “clue”), of certain superficial mycoses e.g., dermatophytoses, may raise a suspicion of fungal infection and warrant a fungal-specific staining. A retrospective analysis of all PAS-stained sections was carried out. All PAS-positive biopsy specimens were assessed for clinical features, histopathological patterns of skin reactions, and presence of histopathological indicators. Our results have shown that out of the total of 361 PAS-stained sections, fungal hyphae were identified in 12 (3.3%) specimens. In 5 (1.4%) cases, the diagnosis of fungal infection was suspected on clinical grounds, while in 7 (1.9%) cases detection of fungi was an unexpected finding. The most frequent type of histopathological pattern was spongiotic, and the most frequent histopathological indicator was the presence of neutrophils within the epidermis. Our results confirm that dermatophytoses may present with clinical and histological non-specific findings. PAS staining represents a relatively cheap and simple fungal-specific staining. It has been suggested that it not only confirms that the selected material is actually invaded, but also reduces the number of false-negative direct reports, where fungi are cultured from a microscopically negative specimen. Apart from a small percentage of positive findings, our results justify the need for routine PAS staining of all clinically and histologically non-specific inflammatory skin conditions.

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Use of CD34 and Factor VIII To Diagnose Hepatocellular Carcinoma on Fine Needle Aspirates

Acta Cytologica ◽

10.1159/000331828 ◽

1998 ◽

Vol 42 (3) ◽

pp. 691-696 ◽

Cited By ~ 27

Author(s):

Shoshana Gottschalk-Sabag ◽

Noemi Ron ◽

Tzipporah Glick

Keyword(s):

Hepatocellular Carcinoma ◽

Factor Viii ◽

Fine Needle ◽

Fine Needle Aspirates

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