scholarly journals New applications of the acridine orange fluorescence staining method: Screening for circulating tumor cells

2017 ◽  
Vol 13 (4) ◽  
pp. 2221-2229 ◽  
Author(s):  
Min Liu ◽  
Ruizhe Li ◽  
Yang Tang ◽  
Jiwu Chang ◽  
Rong Han ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Staining Method ◽  
Fluorescence Staining ◽  
Orange Fluorescence ◽  
Acridine Orange Fluorescence ◽  
New Applications

scholarly journals Acridine Orange Fluorescence Stain for the Diagnosis of Malarial Parasite

1970 ◽  
Vol 16 (2) ◽  
pp. 58-61
Author(s):  
Md A Siddique ◽  
Md A Alam ◽  
Md Tipu Sultan ◽  
Md Q Uddin ◽  
SMA Hossain ◽  
...  
Keyword(s):  
Thin Film ◽  
Acridine Orange ◽  
Malarial Parasite ◽  
Fluorescence Staining ◽  
College Hospital ◽  
Medical College ◽  
Thin Film Preparation ◽  
Orange Fluorescence ◽  
Film Preparation ◽  
Acridine Orange Fluorescence

A total of six hundred suspected cases of malaria (150 from each centre of Rajshahi MedicalCollege Hospital and three thanas under Rajshalhi district viz. Paba, Godagari and Charghat)were examined through both thick and thin film preparation and stained by both Giemsa andacridine orange fluorescence staining respectively. All the slides in both stained preparationwere examined by three competent microbiologists in three different centres. Malarial parasitedetection in cases of Rajshahi Medical College Hospital. Paba, Godagari and Charghat. InGiemsa staining parasites were positive in 14 (9.33%), 16(10.66%),26(17.33%) and 41(27.33%)cases respectively while it was found to be positive in 21(14.00%), 36(24.00%),49(32.66%) and61(40.66%) cases in acridine orange fluorescence staining respectively. In comparison acridineorange fluorescence staining was found to be more sensitive over Giemsa staining especially inlow parasitaemic cases.doi: 10.3329/taj.v16i2.3883TAJ December 2003; Vol.16(2): 58-61

scholarly journals Acridine Orange Fluorescence Staining as a Means of Detecting Sperm-Egg Fusion in Mammals.

1999 ◽  
Vol 16 (3) ◽  
pp. 141-147 ◽  
Author(s):  
Haruo Katayose ◽  
Kaoru Yanagida ◽  
Shinako Hashimoto ◽  
Hiroko Yamada ◽  
Akira Sato
Keyword(s):  
Acridine Orange ◽  
Fluorescence Staining ◽  
Orange Fluorescence ◽  
Acridine Orange Fluorescence

Na+/H+ antiporter of brush border vesicles: studies with acridine orange uptake

1982 ◽  
Vol 242 (6) ◽  
pp. F733-F739 ◽  
Author(s):  
D. G. Warnock ◽  
W. W. Reenstra ◽  
V. J. Yee
Keyword(s):  
Fluorescence Quenching ◽  
Acridine Orange ◽  
Brush Border ◽  
Membrane Vesicles ◽  
Sodium Concentration ◽  
Sodium Influx ◽  
Ph Gradients ◽  
Orange Fluorescence ◽  
Acridine Orange Fluorescence ◽  
Rate Limiting

Fluorescence quenching of acridine orange was used to characterize the generation and collapse of pH gradients by the Na+/H+ antiporter of brush border membrane vesicles prepared from rabbit renal cortex. Quenching was observed when acridine orange, a weak base, was taken up to an acidic intravesicular space. Na+/H+ exchange was examined with both Na+ uptake and efflux studies. Acridine orange fluorescence quenching demonstrated the cation specificity of the Na+/H+ antiporter (i.e., sodium and lithium) and was inhibited by amiloride. Parallel studies with nigericin, a K+/H+ antiporter, demonstrated that acridine orange responded very rapidly to pH gradients. Therefore, acridine orange equilibration was not rate limiting in our studies of the Na+/H+ antiporter. Initial rate measurements were made to obtain kinetic parameters for the Na+/H+ antiporter. In sodium influx studies, the half-maximal rate of acridine orange fluorescence change was obtained with an external sodium concentration of 13.3 +/- 0.5 mM.

Further comments on the distribution of acridine orange fluorescence in P. falciparum–infected erythrocytes

2002 ◽  
Vol 119 (2) ◽  
pp. 311-313 ◽  
Author(s):  
Patrick G Bray ◽  
Kevin J Saliba ◽  
Jill D Davies ◽  
David G Spiller ◽  
Michael R.H White ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Orange Fluorescence ◽  
Acridine Orange Fluorescence
Sign in / Sign up

Export Citation Format

Share Document