scholarly journals Direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation of Solanum trilobatum L.

2016 ◽  
Vol 40 ◽  
pp. 866-877 ◽  
Author(s):  
Jayabalan SHILPHA ◽  
Manoharan JAYASHRE ◽  
Muthiah JOE VIRGIN LARGIA ◽  
Manikandan RAMESH
2021 ◽  
Vol 289 ◽  
pp. 110400
Author(s):  
Eveline Y.Y. Kong ◽  
Julianne Biddle ◽  
Mike Foale ◽  
Bart Panis ◽  
Stephen W. Adkins

2010 ◽  
Vol 9 (44) ◽  
pp. 7453-7461 ◽  
Author(s):  
Kumar Ghimire Bimal ◽  
Soo Seong Eun ◽  
Hye Kim Eun ◽  
Lamsal Kabir ◽  
Yeon Yu Chang ◽  
...  

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 628f-628
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass

An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.


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