Isolation and Characterization of Fungal Species from Spoilt Fruits in Utako Market, Abuja, Nigeria

Red pepper is a spice vulnerable to fungal growth and mycotoxin contamination while in the field and post-harvest during storage. Storage fungi are omnipresent and colonize fast when moisture levels go up, ultimately leading to mycotoxin production. Stored samples of the whole, crushed and ground red pepper in the Southern Indian States and Maharashtra, India were examined to isolate and characterize the prevalent contaminant fungal species. The average moisture content was below 10% for all the red pepper samples and the average total fungal load was less than 1×104 CFU/g. Crushed red pepper showed higher fungal counts than ground and whole red pepper. The identified contaminant mycoflora belonged to the group; Aspergillus, Eurotium sp., Cladosporium sp., Eupenicillium sp., Penicillium sp., Miscellaneous fungi, Neosartorya sp., Curvularia sp., and Hyphopichia sp. Aflatoxigenic Aspergillus flavus was present only in 8.3% of the crushed and 2.54% of whole red pepper samples stored for more than two months.

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Isolation and Characterization of a New Fungal Species, Chrysosporium ophiodiicola, from a Mycotic Granuloma of a Black Rat Snake (Elaphe obsoleta obsoleta)

Journal of Clinical Microbiology ◽

10.1128/jcm.01751-08 ◽

2008 ◽

Vol 47 (4) ◽

pp. 1264-1268 ◽

Cited By ~ 50

Author(s):

S. Rajeev ◽

D. A. Sutton ◽

B. L. Wickes ◽

D. L. Miller ◽

D. Giri ◽

...

Keyword(s):

Fungal Species ◽

Elaphe Obsoleta ◽

Black Rat ◽

Isolation And Characterization ◽

Rat Snake

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Preliminary Studies on Fungal Contamination of Two Rupestrian Churches from Matera (Southern Italy)

Sustainability ◽

10.3390/su12176988 ◽

2020 ◽

Vol 12 (17) ◽

pp. 6988

Author(s):

Stefania Mirela Mang ◽

Laura Scrano ◽

Ippolito Camele

Keyword(s):

Fungal Species ◽

World Heritage Site ◽

Detailed Knowledge ◽

Unesco World Heritage ◽

Isolation And Characterization ◽

Heritage Site ◽

Unesco World Heritage Site ◽

Lecanicillium Psalliotae ◽

Very High

The Sassi, a UNESCO World Heritage Site and its rupestrian churches, are richly decorated and visited by thousands of visitors every year. It is important to preserve this heritage which shows signs of deterioration due to abiotic and/or biotic factors. Aiming to carry out in the future an environmental-friendly restoration, a screening of the fungi present on walls and frescoes of two rupestrian churches “Santa Lucia alle Malve” and “La Madonna dei derelitti” located, respectively, in the “Sasso Caveoso” and in the “Sasso Barisano” was performed. Isolation and characterization of fungal species from investigated sites was carried out. Total genomic DNA (gDNA) was extracted from pure fungal cultures and subsequently utilized in PCRs using primers that amplify a portion of the ribosomal DNA (ITS5/ITS4) or the β-tubulin gene (Bt2a/Bt2b). The amplicons were directly sequenced. Obtained nucleotide sequences were compared to those present in the GenBank (NCBI) showing a very high similarity (99–100%) with the following species: Parengyodontium album, Alternaria alternata, Cladosporium cladosporioides, Lecanicillium psalliotae, Meyerozyma guilliermondii and Botryotrichum atrogriseum. All sequences from this study were deposited in the EMBL database. Detailed knowledge about fungi isolated from stone is indispensable not only to counter/reduce the structural and aesthetic damage but also to protect the health of both guardians and visitors who may develop different pathologies due to the spores diffused in the environment.

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Isolation and Characterization of New Metschnikowia pulcherrima Strains as Producers of the Antimicrobial Pigment Pulcherrimin

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-5-618 ◽

2009 ◽

Vol 64 (5-6) ◽

pp. 405-410 ◽

Cited By ~ 32

Author(s):

Sezai Türkel ◽

Beyza Ener

Keyword(s):

Antimicrobial Activities ◽

Fungal Species ◽

Antagonistic Effect ◽

Its2 Region ◽

Human Pathogens ◽

Trichoderma Spp ◽

Isolation And Characterization ◽

Metschnikowia Pulcherrima ◽

Different Strains

Metschnikowia pulcherrima is a highly effective biocontrol yeast due to its pigment pulcherrimin that accumulates in the cells and in the growth medium. Three different strains of M. pulcherrima were isolated from local grapes. The yeast isolates were characterized on the basis of their biochemical, physiological and ITS1-5.8 s rDNA-ITS2 region. Based on the obtained results, the M. pulcherrima isolates were identifi ed as new strains of M. pulcherrima. Strong antagonistic activities of the M. pulcherrima strains on the human pathogens Proteus vulgaris, Escherichia coli, Candida albicans, Candida parapsilosis, Candida krusei, and Trichosporon mucoides were determined. In addition, antagonistic effects of these M. pulcherrima strains were also tested against Aspergillus fl avus, Aspergillus fumigatus, Aspergillus niger, Trichoderma spp., Paecilomyces spp., and Bipolaris spp. and it was shown that the three different strains of M. pulcherrima also have an antagonistic effect on the growth of these fungal species at different extents. This study showed that all three strains of M. pulcherrima produce the same amount of the pigment pulcherrimin, but their antimicrobial activities on different microorganisms show important variations

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