Genetic Relatedness of Vibrio cholerae O1 and non-O1 by ERIC Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis

2000 ◽  
Vol 3 (4) ◽  
pp. 621-622
Author(s):  
Yuherman . ◽  
Son Radu . ◽  
Gulam Rusul . ◽  
Lum Keang Yeang . ◽  
Ooi Wai Ling . ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Genetic Relatedness ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Vibrio Cholerae O1 ◽  
Polymerase Chain

scholarly journals Epidemiological Investigation ofSalmonella Tileneby Pulsed-Field Gel Electrophoresis and Polymerase Chain Reaction

1997 ◽  
Vol 8 (6) ◽  
pp. 318-322 ◽  
Author(s):  
Chandar M Anand ◽  
Kevin Fonseca ◽  
Ken Longmore ◽  
Robert Rennie ◽  
Linda Chui ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Washington State ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Fingerprint ◽  
Rrna Genes ◽  
23S Rrna ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Pulsed-field gel electrophoresis (PFGE) and DNA fingerprinting by the polymerase chain reaction (PCR) were performed on 11 isolates ofSalmonella tilene. Five strains were from a cluster of human patients, six from sugar gliders and pygmy hedgehogs kept as family pets or from local pet retailers, and one isolate from the first North American case ofS tilenedescribed in Washington State in 1994. The PFGE restriction patterns showed all isolates to be similar. However, PCR using primers to the 16S and 23S rRNA genes ofEscherichia colidemonstrated that the Washington State isolate differed from the rest of the other isolates, which were all similar based upon their DNA fingerprint. This study indicates that reliance on one technique alone may be insufficient to show nuances between strains that are, in many respects, closely related.

Interinstitutional and Intrainstitutional Transmission of a Strain ofAcinetobacter baumanniiDetected by Molecular Analysis Comparison of Pulsed-Field Gel Electrophoresis and Repetitive Sequence–Based Polymerase Chain Reaction

2006 ◽  
Vol 27 (9) ◽  
pp. 981-983 ◽  
Author(s):  
S. Saeed ◽  
M. G. Fakih ◽  
K. Riederer ◽  
A. R. Shah ◽  
R. Khatib
Keyword(s):  
Polymerase Chain Reaction ◽  
Acute Care ◽  
Repetitive Sequence ◽  
Gel Electrophoresis ◽  
Care Facility ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Pulsed-field gel electrophoresis and repetitive sequence-based polymerase chain reaction provided comparable strain discrimination with minor discordance in typingAcinetobacter baumanniiclinical isolates from patients at our hospital and affiliated institutions. Typing revealed a cluster strain with intrainstitutional and interinstitutional spread during the study period. A long-term acute care facility may have been the reservoir.

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