Optimizing Tissue Culture Protocol for In Vitro Shoot and Root Development and Acclimatization of Date Palm (Phoenix dactylifera L.) Plantlets

2022 ◽  
Author(s):  
Najamuddin Solangi ◽  
Mushtaque Ahmed Jatoi ◽  
Ghulam Sarwar Markhand ◽  
Adel Ahmed Abul-Soad ◽  
Muhammad Aslam Solangi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Root Development ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L ◽  
Culture Protocol

scholarly journals Effects of Polyamines And Silver Thiosulphate On Phoenix Dactylifera L. Cv. Quntar Micropropagation And Molecular Analysis.

2021 ◽  
Author(s):  
Ahmed Almayahi
Keyword(s):  
Tissue Culture ◽  
Date Palm ◽  
Genetic Fidelity ◽  
Phoenix Dactylifera ◽  
Silver Thiosulfate ◽  
Practical Applications ◽  
Root Regeneration ◽  
Phoenix Dactylifera L ◽  
Fidelity Assessment

Abstract There are some limitations in the practical applications of in vitro date palm tissue culture, such as low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. The objective of the present study is to determine the effect of polyamines (putrescine "PUT" and spermidine" SPD") and silver thiosulfate (STS) on enhancing propagation of date palm cv Quntar in vitro. Media supplemented with 75 mg L-1 SPD in combination with 10 mgL-1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective in root regeneration and the number of roots per shoot, where the best result 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mgL-1 PUT and 10 mgL-1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 90% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar is beneficial in improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

scholarly journals The Effects of Some Antioxidants on the Growth of Excised Date Palm (Phoenix dactylifera L.) Roots Cultured in Vitro

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 875B-875
Author(s):  
Yousef I. Dlaigan ◽  
A.E. Said ◽  
M.A. El-Hamady
Keyword(s):  
Tissue Culture ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Date Palm ◽  
Root Culture ◽  
Phoenix Dactylifera ◽  
Sodium Metabisulfite ◽  
Phoenix Dactylifera L ◽  
Significant Difference

The objective of this investigation was to study the effects of some antioxidants on the growth and elongation of in vitro-cultured date palm roots. Several trials were conducted to determine the antioxidant and the concentration of antioxidants used in date palm tissue culture. Three types of antioxidants were tested—activated charcoal, polyvinylpyrrolidone (PVP), and sodium metabisulfite (NA2S2O5)—in various concentrations, singly, or in combinations. The medium used for root culture consisted of MS salts mixture, 1/2 modified White's organics, 60% sucrose, 0.004% inositol, 240 mg adenine sulfate/liter, 1.0 mg 2,4-D/liter, and 2.0 mg kinetin/liter. pH was adjusted at 5.7 ± 0.1. Concentrations of activated charcoal tested (in g–liter–1) were: 0.0, 1, 2, 3, 4, 6, 8, 10, and those of PVP (in g–liter–1) were: 0.0, 5, 10, 20, 40 (with or without 4 g activated charcoal/liter). NA2S2O5 concentrations (in mM) tested were: 0.0, 0.2, 0.4, 0.8, 1.6 (with 4 g activated charcoal/liter). The addition of activated charcoal to the culture medium of date palm roots is of vital importance. Four grams per liter gave the best growth and elongation, and there was no significant difference between it and 10 g–liter–1. No growth or elongation occurred in the control. PVP, however, significantly inhibited growth and elongation of cultured roots at all concentrations tested. NA2S2O5, however, stimulated growth and elongation at 0.2 mM. Higher concentrations inhibited growth and elongation.

scholarly journals The Effect of Thidiazuron on Callus and in vitro Shoots Development of Date Palm (Phoenix dactylifera L.) cv. Barhee

2019 ◽  
Vol 32 ◽  
pp. 258-265 ◽  
Author(s):  
Ahmed Z. Al-Asadi ◽  
Aqeel H. Abdulwahid ◽  
Ahmed M.W. Al-Mayahi
Keyword(s):  
Tissue Culture ◽  
Date Palm ◽  
Fruit Trees ◽  
Phoenix Dactylifera ◽  
Optimal Concentration ◽  
Working Paper ◽  
Phoenix Dactylifera L ◽  
In Vitro Shoots ◽  
Southern Iraq

Date palm (Phoenix dactylifera L.) is one of the most important fruit trees spread in southern Iraq, but because of many circumstances decreased their numbers significantly, the propagation of date palm by tissue culture was the best solution for re-preparation of the problem as it was previously, because propagation is accelerated. Summarizes the working paper in an attempt to find the optimal concentration of thidiazuran (TDZ) (0.0 , 0.5 and 1.0) mgl-1, in production of callus and regeneration of shoots, at different times of initial and callus culturing in medium budding. The results showed that the concentration of (0.5) mgl-1 was optimal in all traits under study.

scholarly journals 2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

2021 ◽  
Author(s):  
Emna Baklouti ◽  
Thierry Beulé ◽  
Amèni Nasri ◽  
Amal Ben Romdhane ◽  
Riadh Drira ◽  
...  
Keyword(s):  
Date Palm ◽  
Specific Activity ◽  
Phoenix Dactylifera ◽  
Proline Content ◽  
Epigenetic Changes ◽  
Negative Effects ◽  
Somaclonal Variations ◽  
Phoenix Dactylifera L ◽  
Free Media

Abstract The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L− 1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some significant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 nmol g− 1 to 2973 nmol g− 1 FW when 40 mg L− 1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA); likewise, the specific activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also confirmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8–18.93%.

scholarly journals In vitro Morphogenesis of Arabian Date Palm (Phoenix dactylifera L.)

2014 ◽  
Vol 23 (2) ◽  
pp. 211-219
Author(s):  
Md. Abul Kalam Azad ◽  
Hasnatul Arefin ◽  
Md. Amzad Hossain
Keyword(s):  
Date Palm ◽  
Callus Formation ◽  
Shoot Proliferation ◽  
Phoenix Dactylifera ◽  
Shoot Induction ◽  
In Vitro Morphogenesis ◽  
Shoot Initiation ◽  
Phoenix Dactylifera L ◽  
Young Leaves

After inoculation of young leaves of date palm offshoot required about six months to complete the morphogenesis process. Fourteen weeks were required for embryogenic callus formation under continuous dark condition and nine weeks for shoot initiation (under 16/8 h light/dark). The highest number of explants (80%) produced callus in modified MS containing 5 mg/l 2,4-D + 2 mg/l 2ip. Sixty per cent of explants produced callus in the modified medium containing 5 mg/l 2,4-D + 5 mg/l NAA. while only 50 per cent of the explants formed callus in the same medium when supplemented with only 5 mg/l 2,4-D. The induced calli were transferred to modified MS for shoot proliferation. A combination of two cytokines showed better performance than single ones in shoot induction. The highest percentage (70) of shoot developed in modified MS containing 2 mg/l BAP + 1 mg/l Kn. Forty per cent shoot induction was found in the same medium supplemented with 2 mg/l of BAP. Thirty per cent shoot formed in MS containing 1 mg/l of Kn. The shoots were subcultured at three- four week intervals throughout culture duration. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17522 Plant Tissue Cult. & Biotech. 23(2): 211-219, 2013  (December)

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