Response of Tomato (Lycopersicon esculentum Mill.) Cultivars to MS, Water Agar and Salt Stress in in vitro Culture

Soil salinization is an important abiotic factor negatively affecting plant growth, development and productivity. Fast-growing poplar and willow trees are important plants for bioenergy production demonstrating varying degrees of adaptation to different habitats. The study of salt resistance in different clones of poplars and willows will reveal genotypes that can be planted in saline soils for producing biomass for the bioenergy industry. Therefore, the aim of the study was to investigate the effects of salt stress on poplar plants of clone 'INRA 353-38' (Populus tremula × P. tremuloides) and willow clone 'Zhytomyrska – 1' (Salix sp.) under in vitro culture. For this purpose the plants were cultivated on MS nutrient medium with the addition of sodium chloride in concentrations 25 mM, 50 mM and 100 mM. The control plants were grown on the sodium chloridefree medium. The plant status (with a 4-score scale), the intensity of their growth (by shoot length) and rooting capacity (by the number of roots) were assessed on the 10th and the 30th day of cultivation. The results obtained indicate a high level of sensitivity to sodium chloride of both studied clones under in vitro cultivation. But the willow 'Zhytomyrska – 1' had a higher sensitivity to salt stress comparing to hybrid polar 'ІNRA 353-38' since growth parameters of willow were significantly decreased even under the concentration of sodium chloride 50 mM, and in the case of short term influence (10 days) of the highest concentration of sodium chloride (100 mM) all willow plants terminated their growth and quickly died. The growth parameters of hybrid poplar were declined within a month, mainly under the highest concentration of sodium chloride, but even under such conditions some part of the shoots were able to survive.

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Response of five triticale genotypes to salt stress in in vitro culture

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY ◽

10.3906/tar-1703-103 ◽

2017 ◽

Vol 41 ◽

pp. 372-380 ◽

Cited By ~ 4

Author(s):

İsmail BEZİRĞANOĞLU

Keyword(s):

Salt Stress ◽

In Vitro Culture

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In vitro Regeneration and Agrobacterium-mediated Genetic Transformation of Tomato (Lycopersicon esculentum Mill.)

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v19i1.5004 ◽

1970 ◽

Vol 19 (1) ◽

pp. 101-111 ◽

Cited By ~ 1

Author(s):

Rakha Hari Sarker ◽

Khaleda Islam ◽

M.I. Hoque

Keyword(s):

Lycopersicon Esculentum ◽

Genetic Transformation ◽

In Vitro Regeneration ◽

Leaf Explants ◽

Multiple Shoots ◽

Multiple Shoot ◽

Root Induction ◽

Cotyledonary Leaf ◽

Lycopersicon Esculentum Mill

Agrobacterium-mediated genetic transformation system has been developed for two tomato (Lycopersicon esculentum Mill.) varieties, namely Pusa Ruby (PR) and BARI Tomato-3 (BT-3). Prior to the establishment of transformation protocol cotyledonary leaf explants from the two varieties were cultured to obtain genotype independent in vitro regeneration. Healthy multiple shoot regeneration was obtained from the cut ends of cotyledonary leaf segments for both the varieties on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. The maximum root induction from the regenerated shoots was achieved on half the strength of MS medium supplemented with 0.2 mg/l IAA. The in vitro grown plantlets were successfully transplanted into soil where they flowered and produced fruits identical to those developed by control plants. Transformation ability of cotyledonary leaf explants was tested with Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121, containing GUS and npt II genes. Transformed cotyledonary leaf explants were found to produce multiple shoots on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. Selection of the transformed shoots was carried out by gradually increasing the concentration of kanamycin to 200 mg/l since kanamycin resistant gene was used for transformation experiments. Shoots that survived under selection pressure were subjected to rooting. Transformed rooted plantlets were transferred to soil. Stable expression of GUS gene was detected in the various tissues from putatively transformed plantlets using GUS histochemical assay. Key words: In vitro regeneration, transformation, tomato D.O.I. 10.3329/ptcb.v19i1.5004 Plant Tissue Cult. & Biotech. 19(1): 101-111, 2009 (June)

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IN VITRO CULTURE USED FOR SCREENING SALT STRESS TOLERANT LETTUCE CULTIVARS

Acta Horticulturae ◽

10.17660/actahortic.2012.936.64 ◽

2012 ◽

pp. 477-484

Author(s):

A. Maamouri ◽

Y. Trifa ◽

K. Kouki ◽

K. Aounallah ◽

C. Karmous

Keyword(s):

Salt Stress ◽

In Vitro Culture

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Avaliação da capacidade de regeneração in vitro em tomateiro industrial

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2000000800004 ◽

2000 ◽

Vol 35 (8) ◽

pp. 1523-1529

Author(s):

MIKLÓS FÁRI ◽

GERALDO MILANEZ DE RESENDE ◽

NATONIEL FRANKLIN DE MELO

Keyword(s):

Lycopersicon Esculentum ◽

Lycopersicon Esculentum Mill

Este trabalho teve por objetivo avaliar a capacidade de regeneração das cultivares de tomateiro industrial (Lycopersicon esculentum Mill) IPA-5 e IPA-6, utilizando quatro composições de meio de cultura descritos na literatura e cinco variações de inoculação. Foi testada uma nova variação de inoculação, denominada cotilédone fendido. A maior freqüência de formação de gemas vegetativas foi 100% no caso de IPA-5, e 65% no caso de IPA-6. Para induzir o alongamento de brotos, foram necessários três subcultivos dos explantes apresentando gemas. No caso de IPA-5, o número de brotos obtidos foi maior quando a indução de gemas foi realizada em meio contendo BAP (2,5 mg L-1) e AIA (0,2 mg L-1) seguido de três subcultivos, em meio como zeatina (0,5 mg L-1). Usando esse protocolo, a cultivar IPA-5 produziu uma média de 5,45 brotos alongados a partir do cotilédone fendido. Essa capacidade excedeu significativamente o cotilédone aparado, que produziu 4,4 brotos alongados por explante. No caso de IPA-6, a melhor combinação de meios e método de inoculação produziu 0,87 broto alongado por explante. Os brotos alongados foram enraizados e transferidos para casa de vegetação.

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