Response of five triticale genotypes to salt stress in in vitro culture

Soil salinization is an important abiotic factor negatively affecting plant growth, development and productivity. Fast-growing poplar and willow trees are important plants for bioenergy production demonstrating varying degrees of adaptation to different habitats. The study of salt resistance in different clones of poplars and willows will reveal genotypes that can be planted in saline soils for producing biomass for the bioenergy industry. Therefore, the aim of the study was to investigate the effects of salt stress on poplar plants of clone 'INRA 353-38' (Populus tremula × P. tremuloides) and willow clone 'Zhytomyrska – 1' (Salix sp.) under in vitro culture. For this purpose the plants were cultivated on MS nutrient medium with the addition of sodium chloride in concentrations 25 mM, 50 mM and 100 mM. The control plants were grown on the sodium chloridefree medium. The plant status (with a 4-score scale), the intensity of their growth (by shoot length) and rooting capacity (by the number of roots) were assessed on the 10th and the 30th day of cultivation. The results obtained indicate a high level of sensitivity to sodium chloride of both studied clones under in vitro cultivation. But the willow 'Zhytomyrska – 1' had a higher sensitivity to salt stress comparing to hybrid polar 'ІNRA 353-38' since growth parameters of willow were significantly decreased even under the concentration of sodium chloride 50 mM, and in the case of short term influence (10 days) of the highest concentration of sodium chloride (100 mM) all willow plants terminated their growth and quickly died. The growth parameters of hybrid poplar were declined within a month, mainly under the highest concentration of sodium chloride, but even under such conditions some part of the shoots were able to survive.

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IN VITRO CULTURE USED FOR SCREENING SALT STRESS TOLERANT LETTUCE CULTIVARS

Acta Horticulturae ◽

10.17660/actahortic.2012.936.64 ◽

2012 ◽

pp. 477-484

Author(s):

A. Maamouri ◽

Y. Trifa ◽

K. Kouki ◽

K. Aounallah ◽

C. Karmous

Keyword(s):

Salt Stress ◽

In Vitro Culture

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Response of Tomato (Lycopersicon esculentum Mill.) Cultivars to MS, Water Agar and Salt Stress in in vitro Culture

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2006.170.175 ◽

2005 ◽

Vol 9 (1) ◽

pp. 170-175 ◽

Cited By ~ 2

Author(s):

F. Amini . ◽

A.A. Ehsanpour .

Keyword(s):

Salt Stress ◽

Lycopersicon Esculentum ◽

In Vitro Culture ◽

Lycopersicon Esculentum Mill

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P5CS and HSP 81-2 Gene Expression Profile of Banana (Musa acuminata) in vitro Culture Under Salt Stress Condition

Journal of Plant Sciences ◽

10.3923/jps.2016.91.95 ◽

2016 ◽

Vol 11 (4) ◽

pp. 91-95

Author(s):

S.N. Widiyanto ◽

Kusdianti . ◽

D.S. Diningrat ◽

Iriawati .

Keyword(s):

Gene Expression ◽

Salt Stress ◽

In Vitro Culture ◽

Gene Expression Profile ◽

Expression Profile ◽

Stress Condition ◽

Musa Acuminata ◽

Salt Stress Condition

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Co-treatment effect of triadimefon and salt stress on antioxidant responses, NHX1 and LEA expression in two alfalfa cultivars (Medicago sativa L.) under in vitro culture

Journal of the Korean Society for Applied Biological Chemistry ◽

10.1007/s13765-013-3047-3 ◽

2013 ◽

Vol 56 (4) ◽

pp. 409-417

Author(s):

Leila Arab ◽

Ali Akbar Ehsanpour ◽

Nam Soo Jwa

Keyword(s):

Salt Stress ◽

In Vitro Culture ◽

Medicago Sativa ◽

Treatment Effect ◽

Antioxidant Responses ◽

Medicago Sativa L

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IN VITRO CULTURE REVEALS DIFFERENCES IN THE ORGAN RESPONSIBLE FOR CITRUS RESPONSES TO OSMOTIC OR SALT STRESS

Acta Horticulturae ◽

10.17660/actahortic.2012.961.44 ◽

2012 ◽

pp. 335-341

Author(s):

R.M. Pérez-Clemente ◽

A. Montoliu ◽

C. de Ollas ◽

M.F. López ◽

V. Arbona ◽

...

Keyword(s):

Salt Stress ◽

In Vitro Culture

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Biotechnological assessment of the birch and poplar collection material in salt stress in vitro culture

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-96 ◽

2020 ◽

Author(s):

T.M. Tabatskaya ◽

◽

E.Yu. Amineva ◽

O.S. Mashkina ◽

◽

...

Keyword(s):

Salt Stress ◽

In Vitro Culture ◽

Nutrient Medium ◽

Selective Exposure ◽

In Vitro System ◽

Species Hybrids ◽

Selection Of

A biotechnological assessment (growth, viability, preservation of regeneration potentials) of 16 clones of different birch and poplar species, hybrids and polyploids under saline NaCl conditions in vitro culture was carried out. Three methods of selective exposure to the stressor were tested: chronic (0.2 % NaCl), stair-step (staged increase in the NaCl content in the nutrient medium from 0.2 % to 1.0 %) and hard (1.0 % NaCl). An algorithm for the formation of a selective in vitro system for differentiation and selection of birch and poplar resistant clones under conditions of artificial salinization is proposed.

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Mild Salinity Stimulates Biochemical Activities and Metabolites Associated with Anticancer Activities in Black Horehound (Ballota nigra L.)

Agronomy ◽

10.3390/agronomy11122538 ◽

2021 ◽

Vol 11 (12) ◽

pp. 2538

Author(s):

Mehdi Younessi-Hamzekhanlu ◽

Zahra Dibazarnia ◽

Shahin Oustan ◽

Teniyah Vinson ◽

Ramesh Katam ◽

...

Keyword(s):

Antioxidant Activity ◽

Salt Stress ◽

In Vitro Culture ◽

Salinity Stress ◽

Membrane Integrity ◽

Morphological Characteristics ◽

Culture Conditions ◽

Salinity Level ◽

Total Phenolic

Black horehound (Ballota nigra L.) is one of the most important medicinal plants, as a rich source of health-promoting essential oils and metabolites. Salinity stress affects plant development and alters antioxidant activity and plant metabolite composition. The present research aimed to study the effect of salinity on physiological and biochemical changes and metabolites of B. nigra under greenhouse and in vitro culture conditions. The plants were treated with different concentrations of NaCl (25, 50, 75, 100 mM), and morphological characteristics of the plant were measured. The growth-related traits and soil plant analysis development (SPAD) were decreased both in vivo and in vitro. Additionally, increased salt concentration negatively affected the cell membrane integrity. The total phenolic content and flavonoids of plants growing in the greenhouse increased by 21% at 50 mM of NaCl, but the amounts decreased significantly at higher stress levels (100 mM of NaCl). Antioxidant activity was also measured. Among the NaCl treatments, the most increased DPPH scavenging activities (IC50) under greenhouse and in vitro conditions were detected at mild salinity stress, but the activity significantly decreased in higher salinity treatments (i.e., 75 and 100 mM). In general, with increasing the salinity level to 75 mM, the activities of CAT and APX were significantly upregulated in both greenhouse and in vitro culture conditions. A correlation between total phenolics and flavonoids contents as well as antioxidant activity was obtained. Salinity level caused a shift in the metabolite expression. Mild salinity stress elevated the metabolites involved in anticancer and anti-inflammatory activities, such as β-ionone and caryophyllene oxide. However, the higher salt stress resulted in a significant reduction in their expression. Differential expression of metabolites to various levels of salt stress can be further exploited for the in vitro biosynthesis of metabolites.

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