The Role of Copper in the Growth of Methylosinus trichosporium IMV 3011 and Poly-β-Hydroxybutyrate Biosynthesis

2012 ◽  
Vol 268-270 ◽  
pp. 221-224 ◽  
Author(s):  
Jing Dong
Keyword(s):  
Growth Rate ◽  
Cell Growth ◽  
Copper Concentration ◽  
Methane Monooxygenase ◽  
Lag Time ◽  
Particulate Methane Monooxygenase ◽  
Methylosinus Trichosporium ◽  
Monooxygenase Activity ◽  
Copper Addition

Methanotrophs are aerobic microorganisms that utilize methane as substrates for growth and PHB Biosynthesis. Copper plays an important role in cell growth and PHB biosynthesis. The effect of initial copper concentration on cultivation of M. trichosporium IMV 3011 on methane was investigated. With the addition of 30μmol/L CuSO4•5H2O, PMMO activity improved to 4 times of that without copper addition. The highest density of cultivated cells is 0.48g dry wtL-1, which is 2 times of that without copper addition. The lag time shortened to 15.87h, and the growth rate increased to 0.082h-1.The PHB content increased to 8.3%. It is found that certain initial copper concentration is beneficial to expression of high particulate methane monooxygenase activity, which may contribute to the synthesis of PHB in the cell.

scholarly journals Methanobactin from Methylocystis sp. Strain SB2 Affects Gene Expression and Methane Monooxygenase Activity in Methylosinus trichosporium OB3b

2015 ◽  
Vol 81 (7) ◽  
pp. 2466-2473 ◽  
Author(s):  
Muhammad Farhan Ul-Haque ◽  
Bhagyalakshmi Kalidass ◽  
Alexey Vorobev ◽  
Bipin S. Baral ◽  
Alan A. DiSpirito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
Monooxygenase Activity ◽  
Content Type ◽  
Soluble Methane Monooxygenase ◽  
A Subunit ◽  
Membrane Bound

ABSTRACTMethanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced byMethylosinus trichosporiumOB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. WhenMethylosinus trichosporiumOB3b was grown in the presence of 1 μM CuCl2, expression ofmmoX, encoding a subunit of the hydroxylase component of sMMO, was very low.mmoXexpression increased, however, when methanobactin fromMethylocystissp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated withM. trichosporiumOB3b. IfM. trichosporiumOB3b was grown in the absence of CuCl2, themmoXexpression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure ofMethylosinus trichosporiumOB3b to SB2-Mb had no effect on expression ofmbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2.mbnAexpression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin “piracy” may be commonplace.

Intracytoplasmic membrane formation in Methylomicrobium album BG8 is stimulated by copper in the growth medium

1997 ◽  
Vol 43 (7) ◽  
pp. 672-676 ◽  
Author(s):  
Christine A. Brantner ◽  
Lorie A. Buchholz ◽  
Claudia L. McSwain ◽  
Laura L. Newcomb ◽  
Charles C. Remsen ◽  
...  
Keyword(s):  
Membrane Structure ◽  
Copper Concentration ◽  
Methane Monooxygenase ◽  
Cell Mass ◽  
Sole Source ◽  
Intracytoplasmic Membrane ◽  
Particulate Methane Monooxygenase ◽  
Monooxygenase Activity ◽  
Oxidation Of Methane ◽  
Methylomicrobium Album

Methylomicrobium album BG8 uses methane as its sole source of carbon and energy. The oxidation of methane to methanol is catalyzed by the enzyme methane monooxygenase. Methane monooxygenase activity, intracytoplasmic membrane abundance, and cell mass increased with increasing copper concentration in the medium. When copper was added to copper-deficient cultures, cell mass and intracytoplasmic membrane structure increased. These findings are consistent with the presence of copper in the particulate methane monooxygenase. Methane monooxygenase activity and intracytoplasmic membrane abundance were correlated, suggesting that the methane monooxygenase may be involved in intracytoplasmic membrane proliferation.Key words: Methylomicrobium album BG8, copper, intracytoplasmic membrane, methane monooxygenase.

Particulate methane monooxygenase from Methylosinus trichosporium is a copper-containing enzyme

2002 ◽  
Vol 295 (1) ◽  
pp. 182-186 ◽  
Author(s):  
Jia-Ying Xin ◽  
Jun-Ru Cui ◽  
Xiao-Xue Hu ◽  
Shu-Ben Li ◽  
Chun-Gu Xia ◽  
...  
Keyword(s):  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Methylosinus Trichosporium
Sign in / Sign up

Export Citation Format

Share Document