Electrochemical Behavior of Glucose Oxidase on Gold Electrode Modified by Poly(3, 4-Ethylenedioxy-Thiophene)

Conducting poly (3, 4-ethylenedioxy-thiophene) (PEDOT) thin film was prepared onto gold electrode by electrodepositing from aqueous electrolyte. Glucose oxidase (Gox) was further immobilized on the PEDOT/Au by physical adsorption. The morphology the modified electrode was investigated by FE-SEM. In phosphate buffer medium (0.1mol·L-1 pH 5.5), cyclic voltmetric results indicated that GOx can be immobilized on the PEDOT film, and the biological activation of GOx was retained. A direct electron transfer was found between immobilized GOx and electrode, which suggested that the PEDOT film could not only supply a platform for enzyme immobilization but also offer a effectively electrical contact with the active sites of enzyme.