Preparation and Characterize of Konjac Glucomannan-Based Protein Molecularly Imprinted Polymer

2011 ◽  
Vol 366 ◽  
pp. 460-463
Author(s):  
Zhi Yuan Mi ◽  
Zhuo Ma ◽  
Xiao Li Li ◽  
Pan Wang ◽  
Ying Qing Zhang

Objective: To prepare a novel molecularly imprinted materials for protein recognition. Methods: Konjac glucomannan was used as fundamental materials, after swollen, added 3-Chloro-1,2- epoxypropane and glycerol cross-linked for 3 h at 40 °C. Then added bovine serum albumin (BSA) solution, agitated 1 h at room temperature. Natural dried. Results: After eluted, the imprinted efficiency of the imprinted membrane were (59.27±3.53) % (n=6) with blank membrane as control. The adsorption rate increased from 2 h to 20 h, and reached saturation at 22 to 24 h. The selectively recognition experiment indicated the BSA imprinted membrane have significant selectively recognition abilities for the template protein. Conclusions: konjac glucomannan-based imprinted membrane shows great possibilities to be a promising material for protein molecularly imprinted recognition.

2010 ◽  
Vol 40 (3) ◽  
pp. 282-290
Author(s):  
JingXia YAO ◽  
MinJie GUO ◽  
JianJun MI HuaiFeng XIA ◽  
怀风 宓 ◽  
Ting GAO ◽  
...  

2005 ◽  
pp. 3224 ◽  
Author(s):  
A. Salinas-Castillo ◽  
I. Sánchez-Barragán ◽  
J. M. Costa-Fernández ◽  
R. Pereiro ◽  
A. Ballesteros ◽  
...  

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