Analysis of M Gene of Porcine Epidemic Diarrhea Virus Isolates in Different Regions of Sichuan Province

2013 ◽  
Vol 647 ◽  
pp. 450-455
Author(s):  
Jiang Mei ◽  
Yang Ze Xiao ◽  
Yao Xue Ping ◽  
Peng Bin ◽  
Liu Bo ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Genetic Relationships ◽  
M Gene ◽  
Diarrhea Virus ◽  
Bioinformatics Software ◽  
Flexible Region ◽  
Porcine Epidemic Diarrhea ◽  
Different Strains ◽  
Virus Isolates ◽  
Antigenic Domain

The highly conserved M gene of the virus PEDV that caused pigling diarrheas in 8 different regions of Sichuan was cloned and sequenced to confirm that these diarrheas were caused by virus of at least 4 different strains. Sequence analysis was then conducted to the measured gene sequence to obtain their genetic relationships with the CV777 strain and their positions in the phylogenetic tree. Bioinformatics software DNAStar Protean was utilized to predict the antigenic domain of M gene which encoded M protein of the CV777 strain. Truncation gene corresponded to major antigenic domain was selected for analysis of its secondary structure, flexible region of protein skeleton (protein backbone) and predict the surface accessibility. Online prediction of hydrophilicity of this protein and transmembrane domains were also conducted. All these were done for the purpose of finding out whether the angigenicity changes when mutation of the four cloned unused M genes occurs.

scholarly journals Characterisation of porcine epidemic diarrhea virus isolates during the 2014–2015 outbreak in the Philippines

VirusDisease ◽  
2018 ◽  
Vol 29 (3) ◽  
pp. 342-348 ◽  
Author(s):  
Gemerlyn G. Garcia ◽  
Mark Arman D. Aquino ◽  
Michelle M. Balbin ◽  
Lawrence P. Belotindos ◽  
Jonathan G. Supnet ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
The Philippines ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Virus Isolates

scholarly journals Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China

2016 ◽  
Vol 19 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Ren-feng Li ◽  
Xiang-qin Tian ◽  
Yong Liu ◽  
Jing Xu ◽  
Dong-yang Liu
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Current Knowledge ◽  
Vero Cells ◽  
Immunofluorescence Assay ◽  
Central China ◽  
M Gene ◽  
Diarrhea Virus ◽  
New Variant ◽  
Ultrathin Sections ◽  
Porcine Epidemic Diarrhea

AbstractPorcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.

scholarly journals Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China

2018 ◽  
Vol 12 (12) ◽  
pp. 1124-1133
Author(s):  
Chunyan Jiang ◽  
Xiaoju Zhang ◽  
Jianfeng Han ◽  
Haijian He ◽  
Chaoying Zhang ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Porcine Epidemic Diarrhea Virus ◽  
Eastern China ◽  
Genetic Evolution ◽  
Viral Pathogens ◽  
Diarrhea Virus ◽  
Evolution Analysis ◽  
Strain Vaccine ◽  
Porcine Epidemic Diarrhea ◽  
Virus Isolates

Introduction: Porcine epidemic diarrhea virus (PEDV) is one of the most common viral pathogens causing swine diarrhea. Methodology: We performed a genetic evolution analysis of the S1 gene of endemic PEDV strains in Eastern China. The S1 genes of 37 PEDV-positive samples were amplified and sequenced, and compared to the standard CV777 strain, 120 nucleotides were found to have mutations. Results: The nucleotide and deduced amino acid homologies between the sequences and those of the CV777 strain were 90%–91% and 88.2%–90%, respectively, and their homologies to the vaccine strain were 88.6%–89.7% and 86.2%–87.8%, respectively. Genetic evolution and variation analyses indicated that the 37 PEDV strains belonged to genogroup 2-1, while the CV777 strain, vaccine strain, and earlier Chinese strains all belonged to genogroup 1-1. Conclusions: The newly emerged clinical PEDV strains indicate that the PEDV CV777 vaccine currently used in China may not fully protect pigs from infection with recent epidemic strains, and will require the development of new vaccine strains.

scholarly journals Identifying outbreaks of Porcine Epidemic Diarrhea virus through animal movements and spatial neighborhoods

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gustavo Machado ◽  
Carles Vilalta ◽  
Mariana Recamonde-Mendoza ◽  
Cesar Corzo ◽  
Montserrat Torremorell ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Movements ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea
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