Improving the Yields of Carotene from Red Yeast by Using Ultra High Pressure and its Mutagenic Mechanism

2014 ◽  
Vol 941-944 ◽  
pp. 1016-1020
Author(s):  
Sui Lou Wang ◽  
Hai Xiang Wang ◽  
Zhi Ping Yang
Keyword(s):  
High Pressure ◽  
Acid Level ◽  
Fragment Length Polymorphism ◽  
Survival Curve ◽  
Polymorphism Analysis ◽  
Genetic Quality ◽  
Ultra High Pressure ◽  
Β Carotene ◽  
Mutagenic Mechanism ◽  
Restriction Fragment Length

The R. glutinis NR98 of high-producing β-carotene was treated by ultra high pressure (UHP) of 100~500 MPa for 10~30 min. The survival curve of NR98 treated for 10 min was saddle, which shows that the effect of UHP on this strain maybe was the cumulation of many biology effects. The rate of β-carotene production from mutant G39 (obtained at 300 MPa for 10 min) was increased by 59.87% compared with the initial strain NR98, and its genetic quality was proved to be stable by experiments. The result of restriction fragment length polymorphism analysis suggested that mutant strain G39 was likely to change in nucleic acid level.

scholarly journals Structure of the Chromosome VII Centromere Region in Neurospora crassa: Degenerate Transposons and Simple Repeats

1998 ◽  
Vol 18 (9) ◽  
pp. 5465-5477 ◽  
Author(s):  
Edward B. Cambareri ◽  
Rafael Aisner ◽  
John Carbon
Keyword(s):  
Neurospora Crassa ◽  
Yeast Artificial Chromosome ◽  
Fragment Length Polymorphism ◽  
Artificial Chromosome ◽  
Polymorphism Analysis ◽  
Dna Repeats ◽  
Centromere Region ◽  
Simple Repeats ◽  
Restriction Fragment Length ◽  
Repeat Induced Point Mutation

ABSTRACT DNA from the centromere region of linkage group (LG) VII ofNeurospora crassa was cloned previously from a yeast artificial chromosome library and was found to be atypical ofNeurospora DNA in both composition (AT rich) and complexity (repetitive). We have determined the DNA sequence of a small portion (∼16.1 kb) of this region and have identified a cluster of three new retrotransposon-like elements as well as degenerate fragments from the 3′ end of Tad, a previously identified LINE-like retrotransposon. This region contains a novel full-length but nonmobilecopia-like element, designated Tcen, that is only associated with centromere regions. Adjacent DNA contains portions of a gypsy-like element designated Tgl1. A third new element, Tgl2, shows similarity to theTy3 transposon of Saccharomyces cerevisiae. All three of these elements appear to be degenerate, containing predominantly transition mutations suggestive of the repeat-induced point mutation (RIP) process. Three new simple DNA repeats have also been identified in the LG VII centromere region. While Tcenelements map exclusively to centromere regions by restriction fragment length polymorphism analysis, the defective Tad elements appear to occur most frequently within centromeres but are also found at other loci including telomeres. The characteristics and arrangement of these elements are similar to those seen in theDrosophila centromere, but the relative abundance of each class of repeats, as well as the sequence degeneracy of the transposon-like elements, is unique to Neurospora. These results suggest that the Neurospora centromere is heterochromatic and regional in character, more similar to centromeres of Drosophila than to those of most single-cell yeasts.

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