scholarly journals Isolation and Identification of Indigenous Yeast Strain and Its Potential for Yeast Extract Production

2021 ◽  
Vol 16 (1) ◽  
pp. 8
Author(s):  
S. Mahilrajan ◽  
C. J. Emmanuel ◽  
S. Srivijeindran
Keyword(s):  
Yeast Extract ◽  
Yeast Strain ◽  
Isolation And Identification ◽  
Indigenous Yeast

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

Renewable Plant Waste As Substrates for Enzyme Production, Saccharification and Direct Bioethanol Production by Indigenous Yeast Strain Pichia Exigua

2021 ◽  
Vol 47 (2) ◽  
pp. 306-316
Author(s):  
O.C. Amadi ◽  
C.C. Mbaeke ◽  
T.N. Nwagu ◽  
C.I. Nnamchi ◽  
I.A. Ndubuisi ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Nitrogen Source ◽  
Substrate Concentration ◽  
Yeast Strain ◽  
Enzyme Production ◽  
Culture Conditions ◽  
Single Step ◽  
Total Carbohydrate ◽  
Plant Waste ◽  
Indigenous Yeast

Renewable plant wastes constitute environmental nuisance. Their conversion by enzymes into bioethanol can be beneficial. We investigated the use of renewable plant waste as substrate for enzyme production and hydrolysis of the plant waste for ethanol production using an indigenous yeast strain. Five yeast strains; MCC-1, MCC-2, MCC-3, MCC-4 and MCC-5 were evaluated for production of sugars, α-amylase, glucoamylase and bioethanol using soluble starch. Phylogenetic analysis using partial sequence of the ITS gene classified MCC-4 as Pichia exigua. Proximate composition of plant wastes – cassava, wild yam, mango seed, udara seed and breadfruit were determined. Results showed total carbohydrate of (83.9%) for cassava flour. The ability of yeast to utilize these substrates and the effect of culture conditions (inoculum, pH, nitrogen source and substrate concentration) were also determined. Cassava pulp flour was the best substrate producing reducing sugar (1.471 ± 0.056mg/mL), α-amylase (0.573 ± 0.019U/mL), glucoamylase (1.605 ± 0.119U/mL), and ethanol (4.440 ± 0.014g/L). Culture conditions revealed optimum for inoculum concentration as (1mL), pH (4), nitrogen source (soya bean, 3g/L) and substrate concentration of (8%). Pichia exigua (MCC-4) a natural yeast strain isolated from the soil has the potential for both enzyme and ethanol production in a single step process.

Isolation and identification of a yeast strain involved in the degradation of Marrakech olive mill wastewater

2011 ◽  
pp. 127-137
Author(s):  
Nawal Fakharedine ◽  
Mouna Ouadghiri ◽  
Mohamed Amar ◽  
Peter Winterton ◽  
Mohamed Hafidi ◽  
...  
Keyword(s):  
Yeast Strain ◽  
Olive Mill Wastewater ◽  
Isolation And Identification ◽  
Olive Mill

scholarly journals Isolation and Partial Evaluation of a Potential Indigenous Yeast Strain <i>Pichia kudriavzevii</i> from a Traditional Rice Beer—“Gora” Prepared by the Koloi Tribes of Tripura

2019 ◽  
Vol 09 (09) ◽  
pp. 824-841 ◽  
Author(s):  
Sushanta Ghosh ◽  
Surajit De Mandal ◽  
Nandita Nath ◽  
David L. Kaipeng ◽  
Lovely Rahaman ◽  
...  
Keyword(s):  
Yeast Strain ◽  
Partial Evaluation ◽  
Pichia Kudriavzevii ◽  
Indigenous Yeast ◽  
Traditional Rice

Detection, Isolation and Identification of Osmotolerant Yeasts from High-Sugar Products

1987 ◽  
Vol 50 (6) ◽  
pp. 468-472 ◽  
Author(s):  
MARCO F. G. JERMINI ◽  
OTTO GEIGES ◽  
WILHELM SCHMIDT-LORENZ
Keyword(s):  
Yeast Extract ◽  
Enrichment Culture ◽  
Debaryomyces Hansenii ◽  
Yeast Cells ◽  
Isolation And Identification ◽  
Torulaspora Delbrueckii ◽  
Enrichment Medium ◽  
Zygosaccharomyces Bailii ◽  
Zygosaccharomyces Rouxii ◽  
High Sugar

A simple presence-absence test for detection of small numbers of osmotolerant yeasts in foods was developed. Yeast extract glucose 50 broth [consisting of 0.5% (w/w) yeast extract and 50% (w/w) glucose] was used as enrichment medium and was incubated with agitation at 30°C. The detection was done by (a) microscope and (b) streaking 0.03 ml of enrichment culture on selective yeast extract glucose 50 agar and incubation at 30°C for 5–7 d. If no yeast cells were observed under the microscope within 10 d of incubation, the product sample was judged as “free from osmotolerant yeasts.” In accordance with this method 28 strains of osmotolerant yeasts were isolated from 27 spoiled high-sugar products. Twenty-four strains were identified as Zygosaccharomyces rouxii, 2 Zygosaccharomyces bailii and 1 each as Torulaspora delbrueckii and Debaryomyces hansenii.

Factors Affecting Isolation and Identification of Haemophilus vaginalis (Corynebacterium vaginale)

1979 ◽  
Vol 9 (1) ◽  
pp. 65-71
Author(s):  
Robert K. Bailey ◽  
Jack L. Voss ◽  
Rodney F. Smith
Keyword(s):  
Yeast Extract ◽  
Systems Analysis ◽  
Basal Medium ◽  
Gas Liquid Chromatography ◽  
Isolation And Identification ◽  
Factors Affecting ◽  
Proteose Peptone ◽  
Bifidobacteria Strain ◽  
Reference Strains ◽  
Peptone Yeast Extract Glucose

The rate of isolation of organisms resembling Haemophilus vaginalis (Corynebacterium vaginale) from vaginal specimens was not significantly affected by anaerobic versus carbon dioxide incubation atmospheres or whether specimens were inoculated on isolation media immediately after collection or after a delay of 6 h. Forty-one clinically isolated strains were provisionally divided into 30 H. vaginalis strains and 11 H. vaginalis -like (HVL) strains based on morphological and growth characteristics. The H. vaginalis strains were less reactive in API-20A identification test strips, (Analytab Products, Inc.) using Lombard-Dowell broth, than in a modified basal medium that contained proteose peptone no. 3 (Difco). The numbers and kinds of substrates fermented by 30 clinical and 2 reference strains of H. vaginalis varied among conventional, API, Minitek (Baltimore Biological Laboratory), and rapid buffered substrate fermentation systems. A greater number and variety of carbohydrates were fermented by the 11 HVL strains more consistently in all four test systems. Analysis of volatile and nonvolatile fermentation end products by gas-liquid chromatography did not reveal significant differences between the H. vaginalis and HVL strains. However, the latter group grew in peptone-yeast extract-glucose broth, whereas the H. vaginalis strains did not grow without the addition of starch to peptone-yeast extract-glucose. All of the reference and clinical strains were similar in their susceptibilities to a variety of antimicrobial compounds except sulfonamides, which inhibited the HVL strains and bifidobacteria but not the H. vaginalis strains. Sulfonamide susceptibility or resistance corresponded in part to the H. vaginalis and HVL-bifidobacteria strain reactions on selected conventional fermentation substrates. Susceptibility or resistance to sulfonamides and metronidazole in conjunction with fermentation tests is described to aid in the separation of H. vaginalis from other possibly unrecognized biotypes of H. vaginalis or other vaginal bacteria that presumptively resemble the organism. A human blood medium known as V agar was also of considerable value in distinguishing H. vaginalis from HVL strains, because only the H. vaginalis strains produced diffuse beta-hemolysis on V agar.

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