Isolation and identification of the volatile components of an extruded autolyzed yeast extract

1991 ◽  
Vol 39 (12) ◽  
pp. 2245-2248 ◽  
Author(s):  
Henry V. Izzo ◽  
Chi Tang Ho
Keyword(s):  
Yeast Extract ◽  
Volatile Components ◽  
Isolation And Identification

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

scholarly journals Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Guangqiang Huang ◽  
Jie Liang ◽  
Xiaosi Chen ◽  
Jing Lin ◽  
Jinyu Wei ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Relevant Literature ◽  
Ultra Performance Liquid Chromatography ◽  
Negative Ion ◽  
Volatile Components ◽  
Isolation And Identification ◽  
Fragmentation Patterns ◽  
Nitrogen Containing Compounds

Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.

Detection, Isolation and Identification of Osmotolerant Yeasts from High-Sugar Products

1987 ◽  
Vol 50 (6) ◽  
pp. 468-472 ◽  
Author(s):  
MARCO F. G. JERMINI ◽  
OTTO GEIGES ◽  
WILHELM SCHMIDT-LORENZ
Keyword(s):  
Yeast Extract ◽  
Enrichment Culture ◽  
Debaryomyces Hansenii ◽  
Yeast Cells ◽  
Isolation And Identification ◽  
Torulaspora Delbrueckii ◽  
Enrichment Medium ◽  
Zygosaccharomyces Bailii ◽  
Zygosaccharomyces Rouxii ◽  
High Sugar

A simple presence-absence test for detection of small numbers of osmotolerant yeasts in foods was developed. Yeast extract glucose 50 broth [consisting of 0.5% (w/w) yeast extract and 50% (w/w) glucose] was used as enrichment medium and was incubated with agitation at 30°C. The detection was done by (a) microscope and (b) streaking 0.03 ml of enrichment culture on selective yeast extract glucose 50 agar and incubation at 30°C for 5–7 d. If no yeast cells were observed under the microscope within 10 d of incubation, the product sample was judged as “free from osmotolerant yeasts.” In accordance with this method 28 strains of osmotolerant yeasts were isolated from 27 spoiled high-sugar products. Twenty-four strains were identified as Zygosaccharomyces rouxii, 2 Zygosaccharomyces bailii and 1 each as Torulaspora delbrueckii and Debaryomyces hansenii.

Factors Affecting Isolation and Identification of Haemophilus vaginalis (Corynebacterium vaginale)

1979 ◽  
Vol 9 (1) ◽  
pp. 65-71
Author(s):  
Robert K. Bailey ◽  
Jack L. Voss ◽  
Rodney F. Smith
Keyword(s):  
Yeast Extract ◽  
Systems Analysis ◽  
Basal Medium ◽  
Gas Liquid Chromatography ◽  
Isolation And Identification ◽  
Factors Affecting ◽  
Proteose Peptone ◽  
Bifidobacteria Strain ◽  
Reference Strains ◽  
Peptone Yeast Extract Glucose

The rate of isolation of organisms resembling Haemophilus vaginalis (Corynebacterium vaginale) from vaginal specimens was not significantly affected by anaerobic versus carbon dioxide incubation atmospheres or whether specimens were inoculated on isolation media immediately after collection or after a delay of 6 h. Forty-one clinically isolated strains were provisionally divided into 30 H. vaginalis strains and 11 H. vaginalis -like (HVL) strains based on morphological and growth characteristics. The H. vaginalis strains were less reactive in API-20A identification test strips, (Analytab Products, Inc.) using Lombard-Dowell broth, than in a modified basal medium that contained proteose peptone no. 3 (Difco). The numbers and kinds of substrates fermented by 30 clinical and 2 reference strains of H. vaginalis varied among conventional, API, Minitek (Baltimore Biological Laboratory), and rapid buffered substrate fermentation systems. A greater number and variety of carbohydrates were fermented by the 11 HVL strains more consistently in all four test systems. Analysis of volatile and nonvolatile fermentation end products by gas-liquid chromatography did not reveal significant differences between the H. vaginalis and HVL strains. However, the latter group grew in peptone-yeast extract-glucose broth, whereas the H. vaginalis strains did not grow without the addition of starch to peptone-yeast extract-glucose. All of the reference and clinical strains were similar in their susceptibilities to a variety of antimicrobial compounds except sulfonamides, which inhibited the HVL strains and bifidobacteria but not the H. vaginalis strains. Sulfonamide susceptibility or resistance corresponded in part to the H. vaginalis and HVL-bifidobacteria strain reactions on selected conventional fermentation substrates. Susceptibility or resistance to sulfonamides and metronidazole in conjunction with fermentation tests is described to aid in the separation of H. vaginalis from other possibly unrecognized biotypes of H. vaginalis or other vaginal bacteria that presumptively resemble the organism. A human blood medium known as V agar was also of considerable value in distinguishing H. vaginalis from HVL strains, because only the H. vaginalis strains produced diffuse beta-hemolysis on V agar.

Volatile Components of a Yeast Extract Composition

2006 ◽  
Vol 50 (1) ◽  
pp. 125-131 ◽  
Author(s):  
JENNIFER M. AMES ◽  
GLESNI MAC LEOD
Keyword(s):  
Yeast Extract ◽  
Volatile Components

scholarly journals Isolation and Identification of Volatile Components in Tempe by Simultaneous Distillation-Extraction Method by Modified Extraction Method

2010 ◽  
Vol 1 (2) ◽  
pp. 63-72 ◽  
Author(s):  
Syahrial Syahrial ◽  
M. Muchalal
Keyword(s):  
Extraction Method ◽  
Volatile Components ◽  
Isolation And Identification ◽  
Freeze Concentration ◽  
Extraction Solvent ◽  
Concentration Method ◽  
Extraction Apparatus ◽  
Maximum Water Temperature ◽  
Simultaneous Distillation Extraction ◽  
Maximum Water

An isolation and identification of volatile components in temps for 2, 5 and 8 days fermentation by simultaneous distillation-extraction method was carried out. Simultaneous distillation-extraction apparatus was modified by Muchalal from the basic Likens-Nickerson's design. Steam distillation and benzena as an extraction solvent was used in this system. The isolation was continuously carried out for 3 hours which maximum water temperature In the Liebig condenser was 8 °C. The extract was concentrated by freeze concentration method, and the volatile components were analyzed and identified by combined gas chromatography-mass spectrophotometry (GC-MS). The Muchalal's simultaneous distillation extraction apparatus have some disadvantage in cold finger condenser, and it's extractor did not have condenser. At least 47, 13 and 5 volatile components were found in 2, 5 and 8 days fermentation, respectively. The volatile components in the 2 days fermentation were nonalal, ɑ-pinene, 2,4-decadienal, 5-phenyldecane, 5-phenylundecane, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3-phenyldodecane, 2-phenyldodecane, 5-phenyltridecane, and caryophyllene; in the 5 days fermentation were nonalal, caryophyllene, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3-phenyldodecane, 2-phenyldodecane; and in the 8 days fermentation were ethenyl butanoic, 2-methy1-3-(methylethenyl)ciclohexyl etanoic and 3,7-dimethyl-5-octenyl etanoic.

scholarly journals Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

2020 ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Nancy M. El-Shweihy
Keyword(s):  
Yeast Extract ◽  
Cholesterol Oxidase ◽  
Agitation Speed ◽  
Isolation And Identification ◽  
Box Behnken Design ◽  
Positive Effects ◽  
Wide Range ◽  
Burman Design ◽  
Promising Source ◽  
Plackett Burman Design

Abstract Background: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification of a new microbial source (s) of cholesterol oxidase are very important. Results: The local isolate Streptomyces sp. strain NEAE-94 is a promising source of cholesterol oxidase. It was identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence. The sequencing product had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 in shake flasks was optimized using surface response methodology. The different process parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentrations had the most significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 was 11.03, 27.31 U/mL after Plackett-Burman Design and Box-Behnken design; respectively, with a fold of increase of 6.06 times compared to the production before applying the Plackett-Burman design (4.51 U/mL). Conclusions: Maximum cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4 % (v/v), temperature 37°C, agitation speed 150 rpm, medium volume 50 mL and incubation time 5 days.

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