Factors Affecting Isolation and Identification of Haemophilus vaginalis (Corynebacterium vaginale)

1979 ◽  
Vol 9 (1) ◽  
pp. 65-71
Author(s):  
Robert K. Bailey ◽  
Jack L. Voss ◽  
Rodney F. Smith
Keyword(s):  
Yeast Extract ◽  
Systems Analysis ◽  
Basal Medium ◽  
Gas Liquid Chromatography ◽  
Isolation And Identification ◽  
Factors Affecting ◽  
Proteose Peptone ◽  
Bifidobacteria Strain ◽  
Reference Strains ◽  
Peptone Yeast Extract Glucose

The rate of isolation of organisms resembling Haemophilus vaginalis (Corynebacterium vaginale) from vaginal specimens was not significantly affected by anaerobic versus carbon dioxide incubation atmospheres or whether specimens were inoculated on isolation media immediately after collection or after a delay of 6 h. Forty-one clinically isolated strains were provisionally divided into 30 H. vaginalis strains and 11 H. vaginalis -like (HVL) strains based on morphological and growth characteristics. The H. vaginalis strains were less reactive in API-20A identification test strips, (Analytab Products, Inc.) using Lombard-Dowell broth, than in a modified basal medium that contained proteose peptone no. 3 (Difco). The numbers and kinds of substrates fermented by 30 clinical and 2 reference strains of H. vaginalis varied among conventional, API, Minitek (Baltimore Biological Laboratory), and rapid buffered substrate fermentation systems. A greater number and variety of carbohydrates were fermented by the 11 HVL strains more consistently in all four test systems. Analysis of volatile and nonvolatile fermentation end products by gas-liquid chromatography did not reveal significant differences between the H. vaginalis and HVL strains. However, the latter group grew in peptone-yeast extract-glucose broth, whereas the H. vaginalis strains did not grow without the addition of starch to peptone-yeast extract-glucose. All of the reference and clinical strains were similar in their susceptibilities to a variety of antimicrobial compounds except sulfonamides, which inhibited the HVL strains and bifidobacteria but not the H. vaginalis strains. Sulfonamide susceptibility or resistance corresponded in part to the H. vaginalis and HVL-bifidobacteria strain reactions on selected conventional fermentation substrates. Susceptibility or resistance to sulfonamides and metronidazole in conjunction with fermentation tests is described to aid in the separation of H. vaginalis from other possibly unrecognized biotypes of H. vaginalis or other vaginal bacteria that presumptively resemble the organism. A human blood medium known as V agar was also of considerable value in distinguishing H. vaginalis from HVL strains, because only the H. vaginalis strains produced diffuse beta-hemolysis on V agar.

scholarly journals Studies on Aerobaclllus Polymyxa IV. Factors Affecting the Production of Hydrogenase and the Hydrogenlyase Systems

1953 ◽  
Vol 6 (2) ◽  
pp. 190 ◽  
Author(s):  
WG Crewther
Keyword(s):  
Methylene Blue ◽  
Hydrogen Production ◽  
Yeast Extract ◽  
Magnesium Sulphate ◽  
Gaseous Hydrogen ◽  
Factors Affecting ◽  
Whole Wheat ◽  
Glucose Phosphate ◽  
Production Of Hydrogen ◽  
Peptone Yeast Extract Glucose

Once-washed cells of Aerobacillu8 polymyxa harvested from a medium containing peptone, yeast extract, glucose, phosphate, and magnesium sulphate, did not produce hydrogen from formate, glucose, or pyruvate nor was methylene blue reduced in the presence of gaseous hydrogen. Production of hydrogen from formate could be demonstrated in fermenting whole wheat mash and by once-washed cells of A.polymyxa grown on strained 10 per cent. wheat mash.

Factors Affecting Protease Production by Bacillus stearothermophilus RM-67

1983 ◽  
Vol 46 (12) ◽  
pp. 1020-1025 ◽  
Author(s):  
A. K. CHOPRA ◽  
D. K. MATHUR
Keyword(s):  
Yeast Extract ◽  
Enzyme Production ◽  
Bacillus Stearothermophilus ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Protease Production ◽  
Salt Medium ◽  
Medium Ph ◽  
Factors Affecting ◽  
Rotary Shaker

Amongst the nitrogen sources, tryptone and yeast extract at 0.5% and 0.15% level, respectively, caused maximum enzyme production by Bacillus stearothermophilus RM-67. Addition of sodium chloride (0.5%) to the basal medium enhanced the enzyme production by 63%. Various sugars incorporated into the standardized basal medium proved inhibitory to enzyme elaboration. Maximum enzyme production was observed in the early decline growth phase of the organism in tryptone-yeast extract-salt medium (pH 6.5) when inoculated at 4% level and incubated on a rotary shaker at 55°C for 8 h and subsequently at 45°C up to 24 h.

Nitrification: an old process, a new concern

1971 ◽  
Vol 24 ◽  
Author(s):  
W. H. Verstraete
Keyword(s):  
Culture Medium ◽  
Nitrogen Sources ◽  
Living Cells ◽  
Factors Affecting ◽  
Inhibiting Effect ◽  
Proteose Peptone ◽  
Asparaginase Activity

Some  factors affecting the L-asparaginase activity of E.  aroideae were investigated. Increasing  concentrations of glucose in the culture medium had an inhibiting effect on  the production of L-asparaginase by this microorganism. Buffering of the  culture medium in order to stabilize the pH during growth resulted in a decrease  of the L-asparaginase activity. From the different nitrogen sources examined,  tryptone, proteose peptone nr 2 and nr 3 stimulated the L-asparaginase  production. Toluene treatment of the cells practically destroyed the  L-asparaginase. Acetone dried cells showed an L-asparaginase activity  comparable with the activity of living cells.

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

OBSERVATIONS ON AMYLOLYTIC BACTERIA: III. CULTURAL CONDITIONS INFLUENCING THE BREAKDOWN OF STARCH BY STENOTHERMOPHILIC BACTERIA BELONGING TO BACILLUS STEAROTHERMOPHILUS

1952 ◽  
Vol 30 (4) ◽  
pp. 360-370 ◽  
Author(s):  
Egon Stark ◽  
P. A. Tetrault
Keyword(s):  
Yeast Extract ◽  
Bacillus Stearothermophilus ◽  
Soluble Starch ◽  
The Other ◽  
Ph Changes ◽  
Proteose Peptone ◽  
Cultural Conditions ◽  
Initial Ph ◽  
Commercial Medium ◽  
Agar Media

Thirty-five cultures of Bacillus stearothermophilus hydrolyzed five starches under various cultural conditions. Hydrolysis occurred regardless of the type, brand, or batch of starch; regardless of the initial pH or of the subsequent pH changes of the medium. Starch in broth was better attacked than in agar media. Some cultures hydrolyzed 0.5%, but not 1% starch; others hydrolyzed easily 10% soluble starch. Length of incubation was important. Certain cultures never formed acid or sugar from starch. Dextrinization was a more reliable indication of starch hydrolysis than was the formation of acid or sugar. Soluble starch gave more consistent results in repeated experiments than did nonsoluble starches. The type of protein medium determines strongly the formation of amylase. Trypticase was the best commercial medium, yeast extract came second. The other 10 media yielded fewer amylolytic cultures. Yeast extract added to media enhanced amylase formation, except with trypticase. Tryptose, proteose-peptone, and neopeptone inhibited the growth of most cultures.

scholarly journals Comparison of API and Minitek to Center for Disease Control methods for the biochemical characterization of anaerobes

1976 ◽  
Vol 4 (3) ◽  
pp. 227-231 ◽  
Author(s):  
S L Hansen ◽  
B J Stewart
Keyword(s):  
Disease Control ◽  
Biochemical Characterization ◽  
Clinical Laboratory ◽  
Ease Of Use ◽  
Gas Liquid Chromatography ◽  
Control Methods ◽  
Conventional Tests ◽  
Reference Strains ◽  
Laboratory Time

Two commercially available micromethod multitest systems (API, Analytab Products, Inc., Minitek-Bioquest) were compared with conventional tests suggested by the Center for Disease Control for the identification of anaerobes. Anaerobiosis for the microsystems was achieved using GasPak system (BBL), A total of 175 anaerobes, including 158 clinical isolates and 17 reference strains, were used. Gram morphology, gas-liquid chromatography data, and biochemical reactions from the Center for Disease Control and Virginia Polytechnic Institute anaerobic manuals were used to identify the organisms. The Minitek system included a new anaerobe inoculum broth and two new disks, dextrose without nitrate and nitrate reductase disks. The percentage of correlation of 12 biochemicals using Minitek and 11 biochemicals using the API were compared with the Center for Disease Control reactions. The percentage of correlation of both positive and negative reactions with the API anaerobic strip ranged from 70.8 to 99.4% and with the Minitek from 97.1 to 100%. The microsystems were also evaluated as to the ease of use, adaptabilty to a clinical laboratory, time, and cost.

Enumeration of starter cultures in fermented milks

1996 ◽  
Vol 63 (1) ◽  
pp. 151-158 ◽  
Author(s):  
Hamid B. Ghoddusi ◽  
Richard K. Robinson
Keyword(s):  
Quality Control ◽  
Prussian Blue ◽  
Yeast Extract ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Proteose Peptone ◽  
Different Strains ◽  
Differential Counts

SummarySome media available for the isolation and enumeration of starter cultures employed for the manufacture of cheese, yogurt and bio-yogurt were examined. Reddy's medium or a modification of Elliker's medium was found to be most satisfactory forLactococcusspp., while trypticase phytone yeast (TPY) agar with a mixture of antibiotics proved suitable for the discrete enumeration ofBifidobacteriumspp. The inclusion of Prussian blue (PB) in reinforced clostridial medium or tryptone proteose peptone yeast extract (TPPY) agar gave excellent differential counts for the starter bacteria in yogurt even when the culture was imbalanced, while TPPY (PB) agar allowed the visible separation of all four of the organisms that might be found in a typical bio-yogurt, namelyLactobacillus delbrueckiisubsp.bulgaricus, Streptococcus thermophilus, a,Bifidobacteriumsp. andLb. acidophilus. It was noted that variation among different strains of any given species could change the expected reactions, so for quality control purposes the suggested media may need to be modified to cope with the specific cultures in use.

DECOMPOSITION OF CHLORO-SUBSTITUTED ALIPHATIC ACIDS BY SOIL BACTERIA

1957 ◽  
Vol 3 (2) ◽  
pp. 151-164 ◽  
Author(s):  
H. L. Jensen
Keyword(s):  
Amino Acids ◽  
Yeast Extract ◽  
Soil Bacteria ◽  
Basal Medium ◽  
Soil Extract ◽  
Taxonomic Position ◽  
Vitamin B ◽  
Selective Media ◽  
Aliphatic Acids

Three groups of bacteria capable of decomposing chloro-substituted aliphatic acids were isolated from soil by means of selective media. A group of Pseudomonas-like bacteria (A) decomposed monochloroacetate (and monobromoacetate) readily in media with yeast extract, peptone, or amino acids. They also decomposed α-monochloropropionate with moderate vigor, but had little effect on dichloro-acetate and -propionate, and none on trichloroacetate. A non-sporeforming bacterium of uncertain taxonomic position (B) was able to decompose trichloroacetate in media containing soil extract or vitamin B12, and also in basal medium when associated with vitamin B12-producing strains of Streptomyces. Dichloroacetate was only slightly attacked, and monochloroacetate and α-dichloropropionate not at all. A group of bacteria (C) apparently belonging to Agrobacterium decomposed α-dichloropropionate and dichloroacetate, but was less active towards α-monochloropropionate, and did not attack mono- and tri-chloroacetate. The organisms of groups B and C grew only feebly in ordinary media. The decomposition of monochloroacetate, trichloroacetate, and α-dichloropropionate in soil was accelerated by addition of cell suspensions of groups A, B, and C, respectively. The organisms seemed to be more active in the soil than in vitro.

scholarly journals Experimental Design to Improve Cell Growth and Ethanol Production in Syngas Fermentation by Clostridium carboxidivorans

Catalysts ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 59 ◽  
Author(s):  
Carolina Benevenuti ◽  
Alanna Botelho ◽  
Roberta Ribeiro ◽  
Marcelle Branco ◽  
Adejanildo Pereira ◽  
...  
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Biomass Gasification ◽  
Culture Collection ◽  
Medium Composition ◽  
Syngas Fermentation ◽  
The Cost ◽  
Clostridium Carboxidivorans ◽  
Peptone Yeast Extract Glucose

Fermentation of gases from biomass gasification, named syngas, is an important alternative process to obtain biofuels. Sequential experimental designs were used to increase cell growth and ethanol production during syngas fermentation by Clostridium carboxidivorans. Based on ATCC (American Type Culture Collection) 2713 medium composition, it was possible to propose a best medium composition for cell growth, herein called TYA (Tryptone-Yeast extract-Arginine) medium and another one for ethanol production herein called TPYGarg (Tryptone-Peptone-Yeast extract-Glucose-Arginine) medium. In comparison to ATCC® 2713 medium, TYA increased cell growth by 77%, reducing 47% in cost and TPYGarg increased ethanol production more than four-times, and the cost was reduced by 31%. In 72 h of syngas fermentation in TPYGarg medium, 1.75-g/L of cells, 2.28 g/L of ethanol, and 0.74 g/L of butanol were achieved, increasing productivity for syngas fermentation.

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