TECHNOLOGY AND EQUIPMENT FOR PROCESSING ACTIVATED AGRICULTURAL PLANT WASTE INTO BIOETHANOL

The article is devoted to the development of technology and equipment for the production of bioethanol from agricultural plant waste, activated by the steam explosion method. The value and novelty of research lies in obtaining new data on the effective acidic and enzymatic hydrolysis of activated raw materials, and developing a technology for the conversion of plant raw materials into bioethanol. The studies were carried out on the basis of the Department of Wood Materials Processing of Kazan National Research Technological University (Republic of Tatarstan, Kazan). A pilot plant for the production of bioethanol and the principle of its operation are presented. Pine wood waste and wheat straw (collected in Kukmor region of the Republic of Tatarstan in the period August-September 2021) were used as raw materials. Steam-explosive activation of raw materials was carried out at temperatures of 165 ⁰C and 210 ⁰C for 5 minutes. Acid hydrolysis parameters: H2SO4 concentration - 0.5% and 1.5%, hydromodule 1:15, hydrolysis temperature - 187⁰C, hydrolysis duration - 5 hours. Enzymatic hydrolysis parameters: preparation - Cellulox-A (OOO PO Sibbiopharm, Russia) - 6 and 12 g/kg of raw material, hydrolysis temperature - 45 ⁰C, substrate pH 4.7 (acetate buffer), raw material concentration in the substrate 33 g/l, the duration of hydrolysis is 72 h. Alcoholic fermentation of hydrolysates was carried out at 32-34⁰C using Saccharomyces cerevisiae yeast, fermentation duration 7 h, yeast concentration 25 g/l. The bioethanol yield in % of reducing substances was recalculated after determining the mass yield. It is concluded that the vapor-explosive activation of pine wood at a temperature of 210 ºC makes it possible to obtain by acid hydrolysis and anaerobic fermentation of reducing substances up to 0.26 kg (0.33 l) of ethanol from 1 kg of activated raw materials, and activation of wheat straw at the same temperature allows obtaining up to 0.172 kg (0.218 l) ethanol with 1 kg of activated straw

Cellulose-containing waste recycling using fungi

Accumulation of plant waste is a serious environmental problem. Mushrooms with high cellulolytic activity can process it into valuable products that will be useful in solving various industries and agriculture problems. The enzymes of the cellulolytic complex include 1,4-β-D-glucan-4-glucanohydrolase, exo-1,4-β-glucosidase, cellobiohydrolase, β-glucosidase. 1,4-β-D-glucan-4-glucanohydrolases destroy β-1,4-glycosidic bonds within the chain of cellulose and lichenin polysaccharides. Exoglucanases destroy β-1,3- and β-1,4-glycosidic bonds at the end of the molecule. Cellobiohydrolases cleave β-1,4-glycosidic bonds to form cellobiose and glucose. β-glucosidase complete the process of destruction. Fungi with high cellulolytic activity include both representatives of the Ascomycota and Basidiomycota divisions. Ascomycete Chaetomium globosum produces endoglucanases of two families and 8 cellobiohydrolases. Myceliophthora thermophila also produces endoglucanases and cellobiohydrolases, the most abundant of which is Mt Cel7A. The fungus is a promising producer of thermostable enzymes. Trichoderma reesei has a long history of safe use as a source of highly active cellulolytic enzymes and other valuable metabolites. LPMOs of the cellulolytic fungus Thielavia terrestris are considered auxiliary enzymes, but can negatively affect the main enzymes of the complex. Irpex lacteus also produces LPMO and a complete cellulolytic enzyme complex. The cellulolytic activity of fungi and their ability to grow on cheap substrates can be used to bioconvert plant waste into valuable products. One of the ways to utilize them is to convert into compound feed with a high protein content through the use of starter cultures. The use of mushrooms will increase the content of protein and simple carbohydrates, enrich the feed with fats. Another method is to obtain cellulases, which are widely used in many industries. Thanks to the production of biodiesel and bioethanol from cellulose-containing raw materials it is possible to solve the problem of lack of fuel by replacing energy carriers from non-renewable energy sources with their environmentally friendly counterparts. They are less toxic than diesel and gasoline and are also made from renewable resources.

SOSIALISASI PEMANFAATAN LIMBAH TANAMAN BUNCIS (PHASEOLUS VULGARIS, L) SEBAGAI PUPUK CAIR DI DESA TONGKOK

This community service activity is motivated by the large amount of waste from bean plants in Tongkok Village, Lahat Regency which has not been used optimally, especially as liquid fertilizer. Waste from the bean plant is disposed of, burned and not used . This causes a high volume of waste and environmental pollution. Therefore, the pkm stkip pgri lubuklinggau team tried to socialize the use of bean plant waste (phaseolus vulgaris, l) as liquid fertilizer in tongkok village. This PKM has been carried out by involving partners, namely the Tongkok village community and is enthusiastically followed. The output target to be achieved by the PKM team is to increase community values ​​and publications. In its implementation, it uses theoretical and practical methods starting from the preparation stage, providing materials and practices for making liquid fertilizer for bean plant waste

PEMANFAATAN BATOK KELAPA SEBAGAI MEDIA PEMBUATAN BIO-INSTRUMEN MUSIK

<p>Bio-musical instruments can be defined as musical instruments made from plant wastes in the surrounding <br />environment. One of the plant waste that is easily found in the community is coconut shell. Coconut shells are <br />often overlooked for their use as a creative medium, especially in the field of music. Musical instruments are the <br />main media for creativity in the art of music. The rise of musical instruments or musical instruments with high <br />prices has become one of the inhibiting factors for young people to be creative in the field of music. Seeing this <br />phenomenon, it is deemed necessary to develop people's creativity in making their own musical instruments at <br />economical prices but still able to compete with manufactured musical instruments. In making this bio-<br />instrument, the method used consists of problem identification, preparation, application, and evaluation. <br />Through this method, the results obtained in the use of coconut shell waste into creative media are the Kalimba <br />instrument played by plucking the iron keys as the source of the tone, while the coconut shell is used as the main <br />medium for the sound resonance of the Kalimba instrument. Kalimba is classified into a type of lamellophone <br />instrument, which is a musical instrument that has a tongue or a thin plate. In addition to the easy-to-use <br />manufacturing process, the tools and materials in making these instruments are also easy to find and can even <br />take advantage of used materials. The results of making bio-musical instruments are expected to become a <br />reference and reference for academics and non-academics, regarding how to make musical bio-instruments <br />using coconut shell waste. <br /> <br />Keywords : Bio-Music Intrument, Coconut Shells, Kalimba</p>

EVALUATION OF ENGINEERING PROPERTIES OF THERMAL POWER PLANT WASTE FOR SUBGRADE TREATMENT

Soft cohesive soils have low strength, high plasticity, and a large expansion ratio making them unsuitable as a road subgrade. This study aims to evaluate the potential of power plant waste (fly ash) from the Barapukuria Thermal Power Plant, Dinajpur, Bangladesh to improve the characteristics of such soft cohesive soil. X-ray fluorescence test conducted to classify the power plant fly ash and the type was identified as “Class F” according to “American Association of State Highway and Transportation Officials” and "American Society for Testing and Materials". Laboratory tests were conducted on clay soil obtained from Dinajpur region modified by the collected power plant waste. As the Class F fly ash has low cementing property, 3% cement was added with it. Cement mixed soil was modified with 5%, 10%, 15%, and 20% fly ash respectively. Specific Gravity, Atterberg limits, Modified Proctor Compaction, Unconfined Compressive Strength (UCS) and California Bearing Ratio (CBR) tests were conducted. The study reveals that there is a decrease in specific gravity, dry density, and plasticity index with the addition of power plant waste. On the other hand, there is an increase in optimum moisture content, UCS, and CBR value. UCS and CBR values were found to be improved remarkably. Soaked CBR value of soil is found to be improved from 2.79% to 92.59% when treated with 5% fly ash and 3% cement. The UCS value of this modified soil was 560.36 kPa. The stabilized soil thus obtained meets the requirements for subgrade as specified by the Local Government Engineering Department (LGED)’s design manual (2005), Bangladesh. Since there is a possibility of leaching by dumping a large quantity of fly ash in the pond, the use of fly ash from the power plants to improve soft cohesive soils for road subgrade may be an environment-friendly alternative to its disposal in the ponds.

Surfactant rhamnolipid promotes anaerobic codigestion of excess sludge and plant waste

In order to solve the bottleneck of low methane production in anaerobic co digestion of excess sludge (ES) and plant waste (PW), a new strategy of enhancing hydrolysis and acidification by rhamnolipid (RL) was proposed under thermophilic condition . The results showed that the optimal dosage of RL was 50 g/Kg total suspended solids, and the maximum yield of methane was 198.5 mL/g volatile suspended solid (VSS), which was 2.3 times of that in the control. RL promoted the dissolution of organic matter in the codigestion process of ES and PW, and the higher the dosage of RL, the higher the concentration of soluble chemical oxygen demand (SCOD) in the fermentation broth. When RL was 100 g/Kg, the maximum content of SCOD in fermentation broth was 2,451 mg/L, and the contents of soluble protein and polysaccharide were 593 mg/L and 419 mg/L on 10 d, respectively, which were significantly higher than other groups. In addition, the yield of VFA in RL group was also significantly increased, and acetate and propionate were the main components of VFAs. This research work provides data support for the resource utilization of ES and PW, and expands the application field of RL.

Natural Crude Dye from Cucurbita Pepo Leaves for Dying, Antimicrobial, and Antioxidant Activities

: Owing to environmental concerns, a great attention is given to use plant waste for obtaining natural dyes. Cucurbita pepo (C. pepo) is one of the most abundantly grown seasonal vegetable plants in Pakistan. However, its leaves are of no use after crop season, and consequently, they are usually discarded. Hence, in this study an attempt is made to extract dye from C. pepo leaves for dying textiles and antimicrobial applications. The fractionation of the obtained dye was done by column chromatography while TLC, UV-Vis and FTIR analyses were performed to further evaluate the composition and nature of the obtained dye. The principal constituents of the obtained dye such as alkaloids, tannins, flavonoids, proteins and sugar were identified by chemical tests. The dye was successfully applied for dying of synthetic and cotton cloths which showed resistivity and high color fastness even after successive washings. Interestingly, the obtained dye changed color with change in temperature. For evaluating potential biomedical importance of the obtained dye, antibacterial and antioxidant activities were checked. Effective antioxidants as well as antimicrobial activity against E. coli were observed. From the present study, we concluded that the nature, composition, washing, and thermal stability, as well as the potential biomedical applicability of the obtained natural dye from C. Pepo leaves, can be a better option for commercialization in future.