The Diagnostic Value of the Nitroblue Tetrazolium Dye Reduction Test in Orthopaedic Field

1976 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
Key Yong Kim ◽  
Duk Yun Cho ◽  
Jang Kyu Park ◽  
Byoung Soo Park ◽  
Myoung Jun Cho
Keyword(s):  
Diagnostic Value ◽  
Nitroblue Tetrazolium ◽  
Reduction Test ◽  
Dye Reduction

Decreased Nitroblue Tetrazolium Dye Reduction in the Phagocytes of Patients Receiving Prednisone

PEDIATRICS ◽  
1970 ◽  
Vol 45 (5) ◽  
pp. 861-865
Author(s):  
Denis R. Miller ◽  
Henry G. Kaplan
Keyword(s):  
Chronic Granulomatous Disease ◽  
Nitroblue Tetrazolium ◽  
Quantitative Assay ◽  
Granulomatous Disease ◽  
Host Defenses ◽  
Dye Reduction ◽  
Slide Test ◽  
Intracellular Metabolism

Nitroblue tetrazolium (NBT) dye reduction by leukocytes of 21 patients receiving prednisone was significantly decreased. Nineteen percent of the patients had values similar to those found in children with chronic granulomatous disease, and 57% had heterozygous-range NBT dye reduction. A qualitative NBT dye reduction "slide test" correlated well with the quantitative assay. The uptake of particles by the phagocytes of steroid-treated patients appeared normal. The exact mechanism of corticosteroid action remains unknown. The decreased dye reduction observed in vitro suggests an induced defect of intracellular metabolism which may be related to known alterations of host defenses which occur in patients receiving these hormones.

scholarly journals Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

2012 ◽  
Vol 36 (0E) ◽  
pp. 104-109
Author(s):  
Salameh Sh. Barhoom ◽  
Suha Ali Hussein
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Reduction Test ◽  
Liver Abscesses ◽  
Dye Reduction ◽  
Culture Supernatants

This study was conducted to extract and titrate the leukotoxin of Fusobacteriumnecrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle inSulaimaniyah region. The culture supernatants of these isolates were subjected to thetetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F.necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titermean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp.funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

scholarly journals Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

2012 ◽  
Vol 36 (0E) ◽  
pp. 110-114
Author(s):  
Suha Ali Hussein
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Reduction Test ◽  
Liver Abscesses ◽  
Dye Reduction ◽  
Culture Supernatants

This study was conducted to extract and titrate the leukotoxin of Fusobacterium necrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle in Sulaimaniyah region. The culture supernatants of these isolates were subjected to the tetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F. necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titer mean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp. funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

The N.B.T. Dye Reduction Test in Orthopedic Infections

1974 ◽  
Vol 9 (1) ◽  
pp. 91
Author(s):  
Suck-Hyun Lee ◽  
Kun-Young Park ◽  
Moon-Sik Hahn ◽  
Young-Hee Choi ◽  
Sang-In Kim
Keyword(s):  
Reduction Test ◽  
Dye Reduction
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