scholarly journals Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

2012 ◽  
Vol 36 (0E) ◽  
pp. 110-114
Author(s):  
Suha Ali Hussein
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Reduction Test ◽  
Liver Abscesses ◽  
Dye Reduction ◽  
Culture Supernatants

This study was conducted to extract and titrate the leukotoxin of Fusobacterium necrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle in Sulaimaniyah region. The culture supernatants of these isolates were subjected to the tetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F. necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titer mean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp. funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

scholarly journals Extraction and Titration of Leukotoxins from Fusobacterium necrophorum Isolates Recovered from Bovine Liver Abscesses in Sulaimaniyah Region

2012 ◽  
Vol 36 (0E) ◽  
pp. 104-109
Author(s):  
Salameh Sh. Barhoom ◽  
Suha Ali Hussein
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Reduction Test ◽  
Liver Abscesses ◽  
Dye Reduction ◽  
Culture Supernatants

This study was conducted to extract and titrate the leukotoxin of Fusobacteriumnecrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle inSulaimaniyah region. The culture supernatants of these isolates were subjected to thetetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F.necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titermean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp.funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).

scholarly journals Ribotyping to compare Fusobacterium necrophorum isolates from bovine liver abscesses, ruminal walls, and ruminal contents.

1997 ◽  
Vol 63 (12) ◽  
pp. 4671-4678 ◽  
Author(s):  
S Narayanan ◽  
T G Nagaraja ◽  
O Okwumabua ◽  
J Staats ◽  
M M Chengappa ◽  
...  
Keyword(s):  
Bovine Liver ◽  
Fusobacterium Necrophorum ◽  
Liver Abscesses

scholarly journals Isolation of Sphaerophorus necrophorus from Bovine Liver Abscesses

1967 ◽  
Vol 15 (6) ◽  
pp. 1492-1493
Author(s):  
H. E. Calkins ◽  
L. H. Scrivner
Keyword(s):  
Bovine Liver ◽  
Liver Abscesses

The N.B.T. Dye Reduction Test in Orthopedic Infections

1974 ◽  
Vol 9 (1) ◽  
pp. 91
Author(s):  
Suck-Hyun Lee ◽  
Kun-Young Park ◽  
Moon-Sik Hahn ◽  
Young-Hee Choi ◽  
Sang-In Kim
Keyword(s):  
Reduction Test ◽  
Dye Reduction

The Diagnostic Value of the Nitroblue Tetrazolium Dye Reduction Test in Orthopaedic Field

1976 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
Key Yong Kim ◽  
Duk Yun Cho ◽  
Jang Kyu Park ◽  
Byoung Soo Park ◽  
Myoung Jun Cho
Keyword(s):  
Diagnostic Value ◽  
Nitroblue Tetrazolium ◽  
Reduction Test ◽  
Dye Reduction

Hepatic lesions and bacterial changes in mice during infection of Fusobacterium necrophorum

1977 ◽  
Vol 23 (10) ◽  
pp. 1465-1477 ◽  
Author(s):  
M. M. Garcia ◽  
K. M. Charlton ◽  
K. A. McKay
Keyword(s):  
Mononuclear Cells ◽  
Plasma Cells ◽  
Inflammatory Cells ◽  
Fusobacterium Necrophorum ◽  
Post Inoculation ◽  
Liver Abscesses ◽  
Infected Liver ◽  
Ultrathin Sections ◽  
Hepatic Lesions ◽  
Intraperitoneal Inoculation

Liver abscesses were induced in male albino mice within 1 week after intraperitoneal inoculation of viable Fusobacterium necrophorum LA 19 culture. Fusobacteremia was transitory and reached a peak 2 h after inoculation then sharply declined until its disappearance 24 h post inoculation. By contrast, the number of fusobacteria in the liver increased rapidly during the first 4 h post inoculation and continued to do so less rapidly until the last sampling time (48 h post inoculation). There were small or large areas of necrosis, usually surrounded by inflammatory cells, small focal accumulations of lymphocytes, plasma cells, and macrophages in areas of parenchyma with no degenerations, generalized proliferation of Kupffer cells, and a few accumulations of fibrin and leukocytes on the surface. Ultrathin sections of infected liver tissues revealed both intact and partially degraded F. necrophorum cells enclosed in phagocytic and digestive vacuoles of mononuclear cells. The results indicate that macrophages play a key role in the pathogenesis of liver abscesses.

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