Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors

To identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy. Bilayers supported by hydrophobic materials (PTFE, polycarbonate, nylon, and silanised silver) had optimal resistance (14–19 GΩ) and capacitance (0.8–1.6 μF) values whereas those with low hydrophobicity did not form BLMs (PVDF) or were short-lived (unsilanised silver). The ability of ion channels to function in BLMs was assessed using a method recently reported to improve the efficiency of proteoliposome incorporation into PTFE-supported bilayers. Voltage-gated sodium channel activation by veratridine and inhibition by saxitoxin showed activity for PTFE, nylon, and silanised silver, but not polycarbonate. Bilayers on thicker, more tortuous, and hydrophobic materials produced higher current levels. Bilayers that self-assembled on PTFE filters were the longest lived and produced the most channel activity using this method.

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Membrane supported bilayer lipid membranes array: preparation, stability and ion-channel insertion

Analytica Chimica Acta ◽

10.1016/s0003-2670(02)00139-3 ◽

2002 ◽

Vol 460 (1) ◽

pp. 23-34 ◽

Cited By ~ 35

Author(s):

G Favero ◽

A D’Annibale ◽

L Campanella ◽

R Santucci ◽

T Ferri

Keyword(s):

Ion Channel ◽

Lipid Membranes ◽

Bilayer Lipid Membranes ◽

Bilayer Lipid ◽

Supported Bilayer

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Ion Channel Behavior of Supported Bilayer Lipid Membranes on a Glassy Carbon Electrode

Analytical Chemistry ◽

10.1021/ac000764x ◽

2000 ◽

Vol 72 (24) ◽

pp. 6030-6033 ◽

Cited By ~ 42

Author(s):

Zhengyan Wu ◽

Jilin Tang ◽

Zhiliang Cheng ◽

Xiurong Yang ◽

Erkang Wang

Keyword(s):

Ion Channel ◽

Glassy Carbon Electrode ◽

Glassy Carbon ◽

Lipid Membranes ◽

Bilayer Lipid Membranes ◽

Carbon Electrode ◽

Bilayer Lipid ◽

Supported Bilayer

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Study of the ion-channel behavior on glassy carbon electrode supported bilayer lipid membranes stimulated by perchlorate anion

Materials Science and Engineering C ◽

10.1016/j.msec.2015.05.067 ◽

2015 ◽

Vol 55 ◽

pp. 431-435 ◽

Cited By ~ 2

Author(s):

Zhiquan Zhang ◽

Jun Shi ◽

Weimin Huang

Keyword(s):

Ion Channel ◽

Glassy Carbon Electrode ◽

Glassy Carbon ◽

Lipid Membranes ◽

Bilayer Lipid Membranes ◽

Carbon Electrode ◽

Bilayer Lipid ◽

Supported Bilayer

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Electrochemical Analysis of Alamethicin Reconstituted Planar Bilayer Lipid Membranes Supported on Polypyrrole Membranes

ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems, Volume 2 ◽

10.1115/smasis2011-5038 ◽

2011 ◽

Author(s):

Hao Zhang ◽

Vishnu Baba Sundaresan ◽

Sergio Salinas ◽

Robert Northcutt

Keyword(s):

Energy Conversion ◽

Conducting Polymer ◽

Lipid Membranes ◽

Cell Membranes ◽

Mechanical Energy ◽

Hybrid Membrane ◽

Electrical Impedance Spectroscopy ◽

Bilayer Lipid Membranes ◽

Bilayer Lipid

Conducting polymers possess similarity in ion transport function to cell membranes and perform electro-chemo-mechanical energy conversion. In an in vitro setup, protein-reconstituted bilayer lipid membranes (bioderived membranes)perform similar energy conversion and behave like cell membranes. Inspired by the similarity in ionic function between a conducting polymer membrane and cell membrane, this article presents a thin-film laminated membrane in which alamethicin-reconstituted lipid bilayer membrane is supported on a polypyrrole membrane. Owing to the synthetic and bioderived nature of the components of the membrane, we refer to the laminated membrane as a hybrid bioderived membrane. In this article, we describe the fabrication steps and electrochemical characterization of the hybrid membrane. The fabrication steps include electropolymerization of pyrrole and vesicle fusion to result in a hybrid membrane; and the characterization involves electrical impedance spectroscopy, chronoamperometry and cyclic voltammetry. The resistance and capacitance of BLM have the magnitude of 4.6×109Ω-cm2 and 1.6×10−8 F/cm2.The conductance of alamethicin has the magnitude of 6.4×10−8 S/cm2. The change in ionic conductance of the bioderived membrane is due to the electrical field applied across alamethicin, a voltage-gated protein and produces a measurable change in the ionic concentration of the conducting polymer substrate.

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