Ion Channel Formation in Bilayer Lipid Membranes

2018 ◽  
pp. 278-287
Author(s):  
R. Guidelli ◽  
L. Becucci
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Channel Formation ◽  
Bilayer Lipid

Membrane supported bilayer lipid membranes array: preparation, stability and ion-channel insertion

2002 ◽  
Vol 460 (1) ◽  
pp. 23-34 ◽  
Author(s):  
G Favero ◽  
A D’Annibale ◽  
L Campanella ◽  
R Santucci ◽  
T Ferri
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Supported Bilayer

Ion Channel Behavior of Supported Bilayer Lipid Membranes on a Glassy Carbon Electrode

2000 ◽  
Vol 72 (24) ◽  
pp. 6030-6033 ◽  
Author(s):  
Zhengyan Wu ◽  
Jilin Tang ◽  
Zhiliang Cheng ◽  
Xiurong Yang ◽  
Erkang Wang
Keyword(s):  
Ion Channel ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Carbon Electrode ◽  
Bilayer Lipid ◽  
Supported Bilayer

Micro- and nanofabrication methods for ion channel reconstitution in bilayer lipid membranes

2017 ◽  
Vol 57 (3S2) ◽  
pp. 03EA01 ◽  
Author(s):  
Daisuke Tadaki ◽  
Daichi Yamaura ◽  
Kohei Arata ◽  
Takeshi Ohori ◽  
Teng Ma ◽  
...  
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Channel Reconstitution

scholarly journals Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Thai Phung ◽  
Yanli Zhang ◽  
James Dunlop ◽  
Julie E. Dalziel
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Electrical Impedance Spectroscopy ◽  
Bilayer Lipid Membranes ◽  
Channel Activity ◽  
Supported Bilayers ◽  
Bilayer Lipid ◽  
Filter Materials ◽  
Hydrophobic Materials ◽  
Sodium Channel Activation

To identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy. Bilayers supported by hydrophobic materials (PTFE, polycarbonate, nylon, and silanised silver) had optimal resistance (14–19 GΩ) and capacitance (0.8–1.6 μF) values whereas those with low hydrophobicity did not form BLMs (PVDF) or were short-lived (unsilanised silver). The ability of ion channels to function in BLMs was assessed using a method recently reported to improve the efficiency of proteoliposome incorporation into PTFE-supported bilayers. Voltage-gated sodium channel activation by veratridine and inhibition by saxitoxin showed activity for PTFE, nylon, and silanised silver, but not polycarbonate. Bilayers on thicker, more tortuous, and hydrophobic materials produced higher current levels. Bilayers that self-assembled on PTFE filters were the longest lived and produced the most channel activity using this method.

Sign in / Sign up

Export Citation Format

Share Document