scholarly journals Development of an Analytical System for Ion Channel Proteins Based on Artificial Bilayer Lipid Membranes —Screening of Drug Components that Haveing Side Effects on hERG Channels for Personalized Medicine—

2018 ◽  
Vol 67 (12) ◽  
pp. 749-760
Author(s):  
Maki KOMIYA ◽  
Teng MA ◽  
Daisuke TADAKI ◽  
Ayumi HIRANO-IWATA
Keyword(s):  
Side Effects ◽  
Personalized Medicine ◽  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Channel Proteins ◽  
Ion Channel Proteins ◽  
Analytical System ◽  
Herg Channels

scholarly journals Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray

Micromachines ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 98
Author(s):  
Ryusuke Miyata ◽  
Daisuke Tadaki ◽  
Daichi Yamaura ◽  
Shun Araki ◽  
Madoka Sato ◽  
...  
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Solvent Free ◽  
Bilayer Lipid Membranes ◽  
Related Gene ◽  
Recording Site ◽  
Channel Proteins ◽  
Free Lipid ◽  
Ion Channel Proteins ◽  
Channel Currents

The reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the efficiency of the BLM reconstitution system, we report on a microarray of stable solvent-free BLMs formed in microfabricated silicon (Si) chips, where micro-apertures with well-defined nano- and micro-tapered edges were fabricated. Sixteen micro-wells were manufactured in a chamber made of Teflon®, and the Si chips were individually embedded in the respective wells as a recording site. Typically, 11 to 16 BLMs were simultaneously formed with an average BLM number of 13.1, which corresponded to a formation probability of 82%. Parallel recordings of ion-channel activities from multiple BLMs were successfully demonstrated using the human ether-a-go-go-related gene (hERG) potassium channel, of which the relation to arrhythmic side effects following drug treatment is well recognized.

Membrane supported bilayer lipid membranes array: preparation, stability and ion-channel insertion

2002 ◽  
Vol 460 (1) ◽  
pp. 23-34 ◽  
Author(s):  
G Favero ◽  
A D’Annibale ◽  
L Campanella ◽  
R Santucci ◽  
T Ferri
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Supported Bilayer

Ion Channel Behavior of Supported Bilayer Lipid Membranes on a Glassy Carbon Electrode

2000 ◽  
Vol 72 (24) ◽  
pp. 6030-6033 ◽  
Author(s):  
Zhengyan Wu ◽  
Jilin Tang ◽  
Zhiliang Cheng ◽  
Xiurong Yang ◽  
Erkang Wang
Keyword(s):  
Ion Channel ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Carbon Electrode ◽  
Bilayer Lipid ◽  
Supported Bilayer

Micro- and nanofabrication methods for ion channel reconstitution in bilayer lipid membranes

2017 ◽  
Vol 57 (3S2) ◽  
pp. 03EA01 ◽  
Author(s):  
Daisuke Tadaki ◽  
Daichi Yamaura ◽  
Kohei Arata ◽  
Takeshi Ohori ◽  
Teng Ma ◽  
...  
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Channel Reconstitution

scholarly journals Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Thai Phung ◽  
Yanli Zhang ◽  
James Dunlop ◽  
Julie E. Dalziel
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Electrical Impedance Spectroscopy ◽  
Bilayer Lipid Membranes ◽  
Channel Activity ◽  
Supported Bilayers ◽  
Bilayer Lipid ◽  
Filter Materials ◽  
Hydrophobic Materials ◽  
Sodium Channel Activation

To identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy. Bilayers supported by hydrophobic materials (PTFE, polycarbonate, nylon, and silanised silver) had optimal resistance (14–19 GΩ) and capacitance (0.8–1.6 μF) values whereas those with low hydrophobicity did not form BLMs (PVDF) or were short-lived (unsilanised silver). The ability of ion channels to function in BLMs was assessed using a method recently reported to improve the efficiency of proteoliposome incorporation into PTFE-supported bilayers. Voltage-gated sodium channel activation by veratridine and inhibition by saxitoxin showed activity for PTFE, nylon, and silanised silver, but not polycarbonate. Bilayers on thicker, more tortuous, and hydrophobic materials produced higher current levels. Bilayers that self-assembled on PTFE filters were the longest lived and produced the most channel activity using this method.

Stabilization of bilayer lipid membranes on solid supports by trehalose

1996 ◽  
Vol 39 (2) ◽  
pp. 299-302 ◽  
Author(s):  
T. Hianik ◽  
J. Dlugopolsky ◽  
M. Gyeppessova ◽  
B. Sivak ◽  
H.T. Tien ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Solid Supports ◽  
Bilayer Lipid
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