scholarly journals The Efficacy of Immediate Implantation of Macroporous Poly(N-[2-Hydroxypropyl]-Methacrylamide) Hydrogel after Laceration Spinal Cord Injury in Young Rats

2021 ◽  
Vol 39 (6) ◽  
pp. 1749-1757
Author(s):  
Volodymyr V Medvediev ◽  
Serhii I Savosko ◽  
Ibrahim M Abdallah ◽  
Natalya G Draguntsova ◽  
Viktoria V Vaslovych ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Young Rats ◽  
Cord Injury ◽  
Hydroxypropyl Methacrylamide ◽  
Immediate Implantation

scholarly journals DEPENDENCE OF THE RESTORATIVE EFFECT OF MACROPOROUS POLY(N-[2-HYDROXYPROPYL]-METHACRYLAMIDE HYDROGEL ON THE SEVERITY OF EXPERIMENTAL LACERATIVE SPINAL CORD INJURY

2021 ◽  
Vol 127 (4) ◽  
pp. 8-21
Author(s):  
Ibrahim Abdallah ◽  
Volodymyr Мedvediev ◽  
Nataliya Draguntsova ◽  
Nana Voitenko ◽  
Vitaliy Tsymbaliuk
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Motor Function ◽  
Severe Trauma ◽  
Individual Characteristics ◽  
Male Rats ◽  
Study Results ◽  
Cord Injury ◽  
Hydroxypropyl Methacrylamide ◽  
Immediate Implantation

restoration of the spinal cord function presents a most severe biomedical issue nowadays. The aim of the study was to detect the macroporous poly(N-[2-hydroxypropyl]-methacrylamide hydrogel (PHPMA-hydrogel, HG) restorative effect dependence on the severity of the laceration spinal cord injury in young organisms. The male rats sample (~1-month-old, ~50 g, inbred Wistar line) was represented with 4 experimental groups: 1) spinal cord lateral hemisection at the level of ~Т12–Т13 segments (Sect; n=11); 2) spinal cord lateral hemiexcision ~1 mm long at the similar level (Exc; n=8); 3) spinal cord lateral hemisection at the similar level with immediate implantation of the hydrogel fragment into the trauma region (HGsect; n=11); 4) spinal cord lateral hemiexcision at the similar level with immediate implantation of the hydrogel fragment into the affected region (HGexс; n=6). The motor function and spasticity of the paretic hindlimb was estimated respectively by the technically modified Basso–Beattie–Bresnahan (ВВВ) and Ashworth, conditionally blinded to individual characteristics of all operated animals and previous study results. The observation lasted for ~5 months. The criteria of non-inclusion were as follows: the ipsilateral hindlimb function level in a week after the injury >9 points ВВВ, and the contralateral hindlimb function level during prolonged period ≤14 points ВВВ. The results were interpreted and presented according to the standardized time scale with interpolatory representation of the motor function and spasticity individual level in certain cases. Asymptotic stage differences between the studied groups and subgroups were stated during the first three weeks as well as in 8 weeks and 3 months after the injury. We found out that in a week after injury the motor function level in group Exc made up 0.9±0.5 points ВВВ, in group HGexc — 3.6±1.2 points, in group Sect — 5.9±1.1 points, in group HGsect — 6.0±1.0 points. In 5 months the motor function level in group Sect made up 9.5±1.0 points ВВВ, in group HGsect — 9.5±1.1 points, in group Exc — 0.8±0.3 points, in group HGexc — 4.5±1.8 points. At the same study stage the spasticity level in groups Sect and HGsect was, respectively, 0.8±0.2 and 0.8±0.3 points Ashworth, in group HGexc — 1.8±0.7 points, in group Exc — 3.6±0.3 points. Throughout the study no significant differences in groups Sect and HGsect have been detected, and in groups Exc і HGexc such differences were detected only in 5 weeks after the injury. The considerable difference of spasticity in groups Sect and HGsect was noted in 1 week after the injury, in groups HGexc and Exc — during first 2 months of the experiment. In groups Sect and Exc reliable difference of both motor function and spasticity level was found at all study stages. In groups HGsect and HGexc considerable difference of the motor function level was characteristic at all stages, except for the end of the 1st and 7th weeks, whereas spasticity level differences throughout the study remained insignificant. So, the tested hydrogel in young organisms shows positive effect only with severe trauma stages accompanied with extensive spinal cord defect.

scholarly journals Model of spinal cord lateral hemi-excision at the lower thoracic level for the tasks of reconstructive and experimental neurosurgery

2021 ◽  
Vol 27 (3) ◽  
pp. 33-53
Author(s):  
Volodymyr V. Medvediev ◽  
Ibrahim M. Abdallah ◽  
Natalya G. Draguntsova ◽  
Sergiy I. Savosko ◽  
Viktoria V. Vaslovych ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Rank Order ◽  
Male Rats ◽  
Matched Pairs ◽  
Young Rats ◽  
Cord Injury ◽  
The Mean ◽  
Ashworth Scale

Purpose. To test the model of spinal cord lateral hemiexcision in young rats. Materials and methods. Animals ‒ male rats (age about 1 month, body weight about 50 g, inbred derivatives of the Wistar line); the number of experimental groups is: 1) lateral spinal cord hemisection at the level of segments about T12–T13 (Sect; n=11); 2) lateral spinal cord hemiexcision about 1 mm long at the similar level (Exc; n=8). Assessment of motor Function Index (FI) and the Spasticity Index (SI) of the paretic hindlimb was carried out using the Basso–Beattie–Bresnahan (BBB) scale and Ashworth scale, respectively, in our technical modifications. The non-inclusion criteria: the BBB score above 9 points of FI for the ipsilateral hindlimb in a week after injury ‒and / or BBB score less than or equal to 14 points of FI of the contralateral hindlimb during a long follow-up period (in general, 2 animals in the Sect group, 3 animals ‒ in the Exc group). Asymptotic differences in the timing of testing between subgroups and groups were revealed during the first three weeks of follow-up. Interpolation reproduction of individual values of FI and SI was used in exceptional cases. The total follow-up period was 5 months. Statistical analysis was performed using the Mann-Whitney U Test, Wilcoxon Matched Pairs Test, Spearman’s Rank Order Correlation. For pathomorphological study, the method of silver impregnation of the spinal cord longitudinal sections of the Exc group animals obtained in 5 months after the simulation of injury was used. Results. One week after injury, the FI in the Sect group was 5.9±1.1 according to BBB points, a statistically significant increase in the FI lasted for the first 3 weeks (p<0.05; Wilcoxon Matched Pairs Test), the FI maximum in the group was 10.1±1.1 BBB points, and the FI value at the end of the study was 9.5±1.0 BBB points. In the Exc group, 1 week after injury, the FI was 0.9±0.5 BBB points, during the next week it reached the actual maximum (1.9±0.7 BBB points), by the end of the 5th month it significantly decreased to 0.8±0.3 BBB points (p<0.05; Wilcoxon Matched Pairs Test). One week after injury, the SI value in the Sect group was 0.3±0.1 points according to Ashworth scale, in the Exc group ‒ 0.7±0.1 Ashworth points, a significant increase (p<0.05; Wilcoxon Matched Pairs Test) in SI in the Sect group was noted during the 2nd week and the 2nd month, in the Exc group ‒ during the 2nd and 6th week, as well as the 3rd and 5th month after injury. The SI final and maximal score for the Sect group was 0.8±0.2 Ashworth points, and for the Exc group ‒ 3.6±0.3 Ashworth points. For both groups, there was no correlation between the mean FI value and a significant positive correlation of the mean SI value with the value of the follow-up period (p<0.05; Spearman’s Rank Order Correlation), as well as the absence of correlation between the mean FI and SI values during the total follow-up period. A significant negative correlation (p<0.05; Spearman’s Rank Order Correlation) between individual FI and SI values was found after 1 and 4 weeks, 3 and 5 months after the injury for the Sect group, as well as after 5, 7, 8 weeks and after 3 and 4 months for the Exc group. At all periods of follow-up, the difference in both FI and SI mean values of both groups was significant (p<0.05; the Mann-Whitney U Test). Conclusions. The studied model of spinal cord injury in young rats is the means of choice for testing solid neural transplantation means for the spinal cord injury restorative treatment. The interpretation of data obtained using the BBB scale on models of lateral half spinal cord injury should be carried out with caution, and the methodology for verifying spasticity requires significant improvement. We recommend that the optimal timing for the FI and SI monitoring after lateral half spinal cord injury is 7 days, 14 days and in 1, 2, 3, 4, 5, 6, and 7 months.

scholarly journals Regeneration of dorsal column axons after spinal cord injury in young rats

1998 ◽  
Vol 249 (2-3) ◽  
pp. 135-138 ◽  
Author(s):  
Soki Kikukawa ◽  
Saburo Kawaguchi ◽  
Akira Mizoguchi ◽  
Chizuka Ide ◽  
Morimichi Koshinaga
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Dorsal Column ◽  
Young Rats ◽  
Cord Injury

Glutamate receptor subunit expression after spinal cord injury in young rats

2004 ◽  
Vol 152 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Kwame M Brown ◽  
Jean R Wrathall ◽  
Robert P Yasuda ◽  
Barry B Wolfe
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Glutamate Receptor ◽  
Receptor Subunit ◽  
Young Rats ◽  
Glutamate Receptor Subunit ◽  
Cord Injury ◽  
Subunit Expression

Rapid Functional Recovery after Spinal Cord Injury in Young Rats

2005 ◽  
Vol 22 (5) ◽  
pp. 559-574 ◽  
Author(s):  
Kwame M. Brown ◽  
Barry B. Wolfe ◽  
Jean R. Wrathall
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Functional Recovery ◽  
Young Rats ◽  
Cord Injury

scholarly journals Regeneration of dorsal column axons after spinal cord injury in young rats

1998 ◽  
Vol 31 ◽  
pp. S317
Author(s):  
Soki Kikukawa ◽  
Saburo Kawaguchi ◽  
Akira Mizoguchi ◽  
Chizuka Ide ◽  
Morimichi Koshinaga
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Dorsal Column ◽  
Young Rats ◽  
Cord Injury

Antiapoptotic and neuroprotective effects of mycophenolate mofetil after acute spinal cord injury in young rats

2009 ◽  
Vol 25 (12) ◽  
pp. 1555-1561 ◽  
Author(s):  
Burcak Bilginer ◽  
Mehmet Bulent Onal ◽  
Fırat Narin ◽  
Huseyin Ustun ◽  
Kamer Kilinc ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Mycophenolate Mofetil ◽  
Acute Spinal Cord Injury ◽  
Neuroprotective Effects ◽  
Young Rats ◽  
Cord Injury
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