scholarly journals Autologous non-cultured epidermal cell suspension combined with platelet rich fibrin for the treatment of stable vitiligo: A case series

2019 ◽  
Author(s):  
Adniana Nareswari ◽  
Dendy Zulfikar ◽  
Indah Julianto ◽  
Suci Widhiati
Keyword(s):  
Wound Healing ◽  
Cell Suspension ◽  
Epidermal Cell ◽  
Case Series ◽  
Poor Response ◽  
Platelet Rich Fibrin ◽  
Donor Skin ◽  
Grafting Technique ◽  
Surgical Modality ◽  
Fibrin Membrane

Non-cultured epidermal cell suspension (NCECS) is a relatively new cellular grafting technique for vitiligo. Platelet rich fibrin (PRF) is a platelet and immune concentrate gather on a single fibrin membrane which can be used in conjunction with grafts and has several advantages, such as promoting wound healing, haemostasis, and give better handling properties to graft materials. This study was conducted to determine the efficacy of NCECS combined with PRF in patients with stable vitiligo. Seven patients with stable vitiligo which not responding to topical and phototherapy for more than 12 months were included in the study. The melanocytes were harvested as an autologous melanocyte rich suspension from a donor skin. The non cultured melanocyte transplanted to recipient area that had been superficially dermabraded and smeared with PRF gel. Of all 7 patients, 1 patients showed excellent pigmentation (90-100%), 2 had good repigmentation (60-89%), 1 had fair repigmentation (25-59%) and 3 patients had a poor response (0-24%). The procedure is safe and promising surgical modality for stable vitiligo.

537 Autologous Skin Cell Suspension for the Treatment of Small (≤10% TBSA) Mixed-Depth Burns

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S116-S117
Author(s):  
Jeffrey E Carter ◽  
James H Holmes ◽  
Kevin N Foster ◽  
David J Smith ◽  
Jeffrey W Shupp
Keyword(s):  
Wound Healing ◽  
Cell Suspension ◽  
Safety Data ◽  
Elderly Subjects ◽  
Full Thickness ◽  
Serious Adverse Events ◽  
Impaired Wound Healing ◽  
Burn Wounds ◽  
Skin Cell ◽  
Donor Skin

Abstract Introduction Split-thickness skin grafts (STSGs) have been the standard of care for many decades. Despite their widespread use, STSGs frequently fail. Autologous skin cell suspension (ASCS) is an FDA approved point of care regenerative medicine technology that reduces donor skin requirements without compromising clinical outcomes. ASCS allows for early treatment and less donor skin harvested that may be useful for hard-to-treat anatomical locations, in compromised patients that have risks for impaired wound healing, or elderly patients with thinner skin. We examined ASCS treatment as an adjunct to meshed autografts in adults with small mixed-depth/full-thickness burns. Methods We obtained IRB-approval for a prospective, multi-center, uncontrolled observational study that allowed continued access to ASCS before FDA approval (ClinicalTrials.gov Identifiers: NCT03333941). Subjects with mixed-depth/full-thickness injuries that required skin grafting with a minimum treatment area of 320 cm2 and burns ranging from 5–50% TBSA were eligible for study enrollment. Our analyses included only patients >18 years of age and ≤10% TBSA mixed-depth/full-thickness injuries that had completed the trial. All subjects had ≥1 burn wounds treated with meshed autografts (2:1–4:1) in combination with ASCS. Healing outcomes were accessed following ASCS treatment by direct visualization of each individual wound and included healing, scar outcomes, and safety data. Results Analyses included 20 subjects older than 18 years of age with ≤10% TBSA mixed-depth/full-thickness injuries. Of these, compromised wound healing was seen in 50.0% of subjects. Burn wounds with ≥90% re-epithelialization increased over time, with 62%, 80%, and 100% of wounds achieving closure at Weeks 1, 2, and 8, respectively. Similar results were seen in subjects with comorbidities known to affect wound healing and in elderly subjects despite their risks for impaired healing. Total POSAS patient (37.8 and 35.4) and observer scores were comparable (23.3 and 18.4) at Weeks 12 and 24. Safety events were typical for this patient population, and no serious adverse events occurred for any of the wounds. Conclusions This analysis provides additional information supporting the use of ASCS for the treatment of small, mixed-depth/full-thickness acute thermal burn injuries in adults, notably those with risk factors for impaired wound healing.

scholarly journals Author′s reply: Platelet-rich fibrin as an adjunct to palatal wound healing after harvesting a free gingival graft: A case series

2014 ◽  
Vol 18 (6) ◽  
pp. 684
Author(s):  
ThomasBetsy Sara ◽  
VargheseJothi Mariam ◽  
GiliyarSubraya Bhat ◽  
KulkarniMihir Raghavendra
Keyword(s):  
Wound Healing ◽  
Case Series ◽  
Platelet Rich Fibrin ◽  
Free Gingival Graft ◽  
Gingival Graft

Autologous, Noncultured Epidermal Cell Suspension Grafting in the Management of Mechanically and Chemically Induced Leukodermic Scars

2015 ◽  
Vol 19 (5) ◽  
pp. 488-493 ◽  
Author(s):  
Nikki S. Vyas ◽  
Kelsey L. Lawrence ◽  
James L. Griffith ◽  
Iltefat H. Hamzavi
Keyword(s):  
Cell Suspension ◽  
Epidermal Cell ◽  
Case Series ◽  
Prognostic Indicator ◽  
The Third ◽  
Small Case Series ◽  
Chemically Induced ◽  
Transplant Procedure ◽  
Skin Phototype

Background: Melanocyte-keratinocyte transplant procedure (MKTP) successfully repigments postburn leukodermic scars. Objective: To further investigate the efficacy and limitations of MKTP for treatment of mechanically and chemically induced leukodermic scars. Methods: Ten patients with mechanically or chemically induced, depigmented or hypopigmented scars were preoperatively evaluated with Wood’s light examination, treated with MKTP, and followed for at least 6 months, with monitoring of repigmentation and colour matching. Results: Nine patients attended at least 6 months of follow-up. Six patients showed no fluorescence of scars under Wood’s lamp. All patients whose lesions didn’t fluoresce displayed less than 50% repigmentation, with 5 of 6 attaining 0% to 24%. Of the 3 patients displaying bright or some fluorescence, more than 95% repigmentation was achieved in 2 patients (skin phototypes V and VI), while less than 24% was attained for the third (skin phototype II). Conclusions: In this small case series, lack of fluorescence in leukodermic scars may be a useful negative prognostic indicator for MKTP, but additional trials are needed to verify that this is not due to melanocompetency.

scholarly journals Platelet-rich fibrin as an adjunct to palatal wound healing after harvesting a free gingival graft: A case series

2014 ◽  
Vol 18 (3) ◽  
pp. 399 ◽  
Author(s):  
BetsySara Thomas ◽  
JothiM. Varghese ◽  
GiliyarSubraya Bhat ◽  
MihirRaghavendra Kulkarni
Keyword(s):  
Wound Healing ◽  
Case Series ◽  
Platelet Rich Fibrin ◽  
Free Gingival Graft ◽  
Gingival Graft
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