Human herpes virus 8 antibodies in HIV-positive patients in Surabaya, Indonesia

Background: Human herpesvirus 8 (HHV-8) infection is etiologically related to Kaposi’s sarcoma. Antibodies directed against HHV-8 can be detected in 80%–95% of HIV-seropositive patients with KS. HHV-8 serological tests have been done in several countries in Southeast Asia such as Malaysia, and Thailand however no serological data is available in Indonesia. This study was to examine the presence of HHV-8 antibodies in HIV-positive patients in Surabaya, Indonesia. Material and methods: Ninety-one serum samples were collected from HIV-positive patients in Surabaya, Indonesia. Human immunodeficiency virus-positive serum samples were collected from 10 homosexual men, 25 intravenous drug users (IVDUs) and 56 heterosexuals. Serums were then tested for the presence of HHV-8 antibody by using sandwich ELISA (Abbexa Ltd, Cambridge, UK). Results: The total of 91 HIV-infected were testing with antibodies to HHV-8 using enzyme-linked immunosorbent assay. Antibodies of HHV-8 were detected in 7/91 (7.7%) of the samples. According to a gender, six men (85.7%) and a women (14.3%) were positive of HHV-8 antibodies. No correlation regarding the gender and age from this study. The antibodies of HHV-8 was detected among intravenous drug users (IVDUs) men 5/7 (42.8%) and 2/7 (28.6%) from homosexual and heterosexual, respectively. Conclusion: This study found the presence of HHV-8 antibodies in 7.7% of patients in Surabaya, Indonesia. This finding was higher more than Southeast Asian countries. The patients with a positive result could suggest measures to prevent HHV-8 infection.

Download Full-text

Prevalence of human herpesvirus-8 among HIV-infected patients, intravenous drug users and the general population in Iran

Sexual Health ◽

10.1071/sh15192 ◽

2016 ◽

Vol 13 (3) ◽

pp. 295 ◽

Cited By ~ 8

Author(s):

Rezvan Kakavand-Ghalehnoei ◽

Zabihollah Shoja ◽

Alireza Najafi ◽

Mostafa Haji Mollahoseini ◽

Shohreh Shahmahmoodi ◽

...

Keyword(s):

General Population ◽

Drug Users ◽

Blood Donors ◽

Human Herpesvirus ◽

Intravenous Drug ◽

Human Herpesvirus 8 ◽

Intravenous Drug Users ◽

Herpesvirus 8 ◽

Sexual Behaviours ◽

Polymerase Chain

Studies looking at the frequency of human herpesvirus-8 (HHV-8) among Iranian blood donors have produced conflicting results. The aim of this study was to investigate the prevalence of HHV-8 DNA by using polymerase chain reaction methods among 168 healthy individuals, 60 intravenous drug users and 100 HIV-infected patients from Iran. The prevalence of HHV-8 was significantly higher among intravenous drug users (13.3%) compared with the general population (3.6%; P = 0.017). The HHV-8 genome was mostly detected among intravenous drug users who displayed high-risk sexual behaviours. Moreover, the HHV-8 genome was also detected in 8% of HIV-infected patients. The present study findings support the likelihood that the transmission of HHV-8 is via a sexual route in the Iranian population.

Download Full-text

Pregnancy outcome among HIV positive and negative intravenous drug users

European Journal of Obstetrics & Gynecology and Reproductive Biology ◽

10.1016/s0301-2115(97)02699-7 ◽

1997 ◽

Vol 72 (2) ◽

pp. 169-174 ◽

Cited By ~ 14

Author(s):

Anna Bucceri ◽

Laura Luchini ◽

Laura Rancilio ◽

Emilio Grossi ◽

Gabriele Ferraris ◽

...

Keyword(s):

Pregnancy Outcome ◽

Drug Users ◽

Intravenous Drug ◽

Hiv Positive ◽

Intravenous Drug Users

Download Full-text

Depressive disorders and personality variables in HIV positive and negative intravenous drug-users

Journal of Affective Disorders ◽

10.1016/s0165-0327(00)00269-x ◽

2001 ◽

Vol 65 (1) ◽

pp. 45-53 ◽

Cited By ~ 24

Author(s):

Cesare Turrina ◽

Alessandra Fiorazzo ◽

Agata Turano ◽

Paolo Cacciani ◽

Cristiana Regini ◽

...

Keyword(s):

Drug Users ◽

Depressive Disorders ◽

Intravenous Drug ◽

Hiv Positive ◽

Intravenous Drug Users ◽

Personality Variables

Download Full-text

Development of a Measure of Nurses' Aids Attitudes and Conservative Views

Psychological Reports ◽

10.2466/pr0.1994.74.3.1043 ◽

1994 ◽

Vol 74 (3) ◽

pp. 1043-1048 ◽

Cited By ~ 12

Author(s):

Melinda Harrison ◽

Marcelline R. Fusilier ◽

Joel K. Worley

Keyword(s):

Factor Analysis ◽

Drug Users ◽

Coefficient Alpha ◽

Intravenous Drug ◽

Hiv Positive ◽

Intravenous Drug Users ◽

Short Scale ◽

Family Issues ◽

Attitudes Toward Homosexuals

The literature concerning measurement of nurses' attitudes toward AIDS was reviewed. A short scale to measure nurses' attitudes toward AIDS was developed and evaluated. The instrument has three subscales on (a) willingness to care for those who are HIV positive, homosexuals, and intravenous drug users as patients, (b) attitudes toward homosexuals and intravenous drug users, and (c) general conservative views on religious, political, and family issues. The three subscales were supported by results of a factor analysis. Coefficient alpha ranged from .69 to .92.

Download Full-text

Differences in Mortality Rates and Causes of Death between HIV Positive and HIV Negative Intravenous Drug Users

International Journal of Epidemiology ◽

10.1093/ije/22.2.315 ◽

1993 ◽

Vol 22 (2) ◽

pp. 315-320 ◽

Cited By ~ 46

Author(s):

ANNE ESKILD ◽

PER MAGNUS ◽

SVEN OVE SAMUELSEN ◽

CHRISTIAN SOHLBERG ◽

PETER KITTELSEN

Keyword(s):

Drug Users ◽

Causes Of Death ◽

Mortality Rates ◽

Intravenous Drug ◽

Hiv Positive ◽

Intravenous Drug Users ◽

Hiv Negative

Download Full-text

Detection of a Novel DNA Virus (TT Virus) in Semen of HIV-Positive Intravenous Drug Users

Fertility and Sterility ◽

10.1016/s0015-0282(00)00943-2 ◽

2000 ◽

Vol 74 (3) ◽

pp. S82

Author(s):

T Persico ◽

V Savasi ◽

C Lanzani ◽

R Tuveri ◽

C Bréchot ◽

...

Keyword(s):

Drug Users ◽

Intravenous Drug ◽

Hiv Positive ◽

Intravenous Drug Users ◽

Dna Virus ◽

Tt Virus

Download Full-text

Adherence to antiretroviral therapy in HIV-positive, male intravenous drug users in Pakistan

Eastern Mediterranean Health Journal ◽

10.26719/2018.24.3.237 ◽

2018 ◽

Vol 24 (03) ◽

pp. 237-242 ◽

Cited By ~ 2

Author(s):

Bakht Ali ◽

Nighat Nisar ◽

Faryal Nawab

Keyword(s):

Antiretroviral Therapy ◽

Drug Users ◽

Intravenous Drug ◽

Hiv Positive ◽

Intravenous Drug Users

Download Full-text

The use of complementary serological and molecular testing for blood-borne pathogens and evaluation of socio-demographic characteristics of intravenous drug users on substitution therapy from Sumadia district of Serbia

Vojnosanitetski pregled ◽

10.2298/vsp170814129b ◽

2019 ◽

Vol 76 (6) ◽

pp. 587-592

Author(s):

Nemanja Borovcanin ◽

Elizabeta Ristanovic ◽

Milena Todorovic ◽

Milica Borovcanin ◽

Mirjana Jovanovic ◽

...

Keyword(s):

Drug Users ◽

Pneumocystis Carinii ◽

Pathogen Transmission ◽

Intravenous Drug ◽

Enzyme Linked Immunosorbent Assay ◽

Molecular Testing ◽

Intravenous Drug Users ◽

Substitution Therapy ◽

Negative Results ◽

Positive Results

Background/Aim. Intravenous drug users (IDUs) are still a high risk-group for cross-reacting blood-borne infections, for vertical pathogen transmission as well as for potentially blood/plasma donation (especially as ?paid? donors). The aim of our study was to establish the profile of opiate addict and prevalence of blood-borne pathogens ? Hepatitis B virus (HBV), Hepatitis C virus (HCV) and Human Immunodeficiency Virus (HIV) among 99 patients on substitution therapy with methadone and buprenorphine from Sumadia District. Methods. The Treatment Demand Indicator (TDI) of Pompidou-questionnaire was used to assess the history of drug abuse and risk behavior. All blood samples were tested for HBV surface antigen (HBsAg), anti-HCV antibody (anti-HCV) and HIV antigen/antibody (HIVAg/ Ab) by Enzyme-Linked ImmunoSorbent Assay (ELISA) or Chemiluminescent Immuno-Assay (CIA). Investigations were also performed for HBV, HCV and HIV by molecular testing ? Polymerase Chain Reaction (PCR) method. Results. The majority of patients were males (81.8%), median age 32 (19?57) years, lived in a city (99%), unemployed (58.6%), with finished secondary school (67.7%), unsafe injecting practices (34.3%) and never previously tested for HBV (39.4%), HCV (36.4%) nor HIV (28.3%); only 4% of them previously got HBV-vaccine. The complementary testing resulted with following results: HBV ELISA/CIA and PCR negativity for 66 patients and positive results (by ELISA/CIA and PCR) for 19 patients. However, a difference was observed in the ELISA/CIAnegative/ PCR-positive result for 12 and ELISA/CIApositive/ PCR-negative for two patients respectively. Further, the negative results for HCV (ELISA/CIA and PCR testing) were found in 15 IDUs and positive results (using both methods) were found in 58 patients. Different results for ELISA/CIA-negative / PCR-positive results were found in 11 IDUs and ELISA/CIA-positive/PCR ? negative results were found in 15 patients. All investigated IDUs were negative for HIV (ELISA/CIA and PCR testing) and for pathogens of opportunistic infection (Cryptococcus neoformans; Pneumocystis carinii; PCR testing), as well as for West Nile Virus (PCR testing). Just one IDU was positive for syphilis (ELISA and confirmatory testing). Conclusion. Our study demonstrated that the positivity for HBV and HCV is still very high (33.4% and 84.8%, respectively) in IDUs. Thus, we suggest that drug users have to be periodically screened using a complementary serological/molecular testing ? concerning differences/discrepancies in the results obtained using these methods.

Download Full-text