scholarly journals OA02.11. Alpha‑amylase inhibitory property and antioxidant potential of Saraca indica and Polyalthia longifolia bark extracts

2013 ◽  
Vol 32 (5) ◽  
pp. 17
Author(s):  
P Gayathri ◽  
GP Jeyanthi
2021 ◽  
Vol 68 ◽  
pp. 35-51
Author(s):  
Sumaira Mumtaz ◽  
Raziya Nadeem ◽  
Raja Adil Sarfraz ◽  
Muhammad Shahid

Synthesis of nanoparticles of transition metals by using medicinal plants has been outstreched in recent years because of the characteristic features which are embodied in the end product. This work is proceded with the aim to synthesize and optimize copper nanoparticles (CuNPs-Pl) using aqueous extract of Polyalthia longifolia leaves (PlL) for characterization and evaluation of antimicrobial and antioxidant potential. The synthesis of CuNPs-Pl was confirmed by visual inspection of the dark brown residues in the reaction flask and via absorption band around 580nm by UV/Visible spectroscopy. Synthesis process was optimized through investigation of environmental variables. FTIR analysis was carried out for both PIL and CuNPs-Pl which identified the presence of alkanes, alcoholic, and aldehydic groups in the PlL and their encapsulation on the copper surface. The synthesized CuNPs-Pl were found to be spherical and rod shaped, and polydispersed when investigated through SEM study. Similarly, these nanoparticles had monoclinic structure and crystalline nature when analyzed by XRD. Moreover, these nanoparticles showed metallic form when EDX examination was done. Further, biological activities were performed. The synthesized nanoparticles showed considerable inhibition zones against Escherichia coli (12mm), Bacillus subtilis (11mm), Aspergillus niger (10mm), and Schyzophyllum commune (16mm) which depicted their powerful antibacterial and antifungal activity. Likewise, CuNPs-Pl were effeciently able to quench free radicals as indicated from the 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), and nitric oxide (NO) assays by exhibiting 86.32, 50.45, and 48.23% inhibition respectively. Thus, the contemporary work has substantiated that low cost CuNPs-Pl can be highly proficient alternate or substitute of synthetic formulations.


2021 ◽  
pp. 113-122
Author(s):  
N. P. G. D. Navoda ◽  
M. D. W. Samaranayake ◽  
S. L. Liyanage ◽  
H. M. T. Herath ◽  
J. M. J. K. Jayasinghe

Objective: To develop vacuum dried (VD) Ambarella fruit powder and Ambarella fruit incorporated soup mix from large and miniature Ambarella and investigation of antioxidants and anti-diabetic properties. Methods: Ethanolic extracts of two Ambarella varieties were analyzed for antioxidant potential in terms of total phenolic content (TPC), total flavonoid content (TFC), ferrous reducing antioxidant power (FRAP), radical scavenging activities of DPPH and ABTS and α-amylase inhibitory activity. Results: Fresh dwarf Ambarella showed a significantly (p<0.05) high TPC (3.35±0.10 mgGAE/g) while fresh large Ambarella showed a significantly (p<0.05) high FRAP (0.71±0.13 mgTE/g) and DPPH (3.57±0.31 mg TE/g). In comparison of ethanolic extracts of VD Ambarella powders, a significantly (p<0.05) higher antioxidant potential in terms of FRAP (4.19±0.06 mgTE/g) exhibited in dwarf variety while significantly (p<0.05) higher ABTS (4.03±0.27 mgTE/g) and DPPH (3.00±0.49mgTE/g) exhibited in large Ambarella. Alpha amylase inhibition activities of fresh fruits of large and dwarf were 46.30±4.07% and 49.55±3.18% where as in VD powders were 27.59±5.03% and 15.58±5.86% respectively. An instant soup mixture was developed incorporating 20% of VD powder from large variety due its abundance. The antioxidant potential of ethanolic extract of the soup mixture in terms of TPC, TFC, ABTS, and FRAP were 0.55±0.00 mgGAE/g, 0.04±0.00 mgQE/g, 1.65±0.06 mgTE/g, and 0.04±0.15 mgTE/g. Alpha amylase inhibition activity of soup mixture was 39.49±0.29%. Conclusion: The both types of fresh fruits exhibited higher antioxidant potential except FRAP and higher anti-amylase inhibition than VD Ambarella powder.


2020 ◽  
Vol 15 (3) ◽  
pp. 151-162
Author(s):  
Audrey Judith Mbang Mbarga ◽  
Cunégonde Blanche Etoundi Omgba ◽  
Rodolphe Djessissem ◽  
Marie Modestine Kana Sop

Le présent travail a pour but d’apporter une contribution à l’amélioration du traitement contre le diabète. Pour ce faire, trois variétés de  champignons comestibles du genre pleurote ont été mélangées (ou formulées) afin d’étudier le potentiel antioydant des extraits bruts issus de ces champignons formulés par les tests DPPH, FRAP et Folin et d’en déterminer ensuite l’influence de ces extraits sur l’activité de l’alpha amylase. En termes de résultats, on a obtenu 4 formulations et 16 extraits. Pour ce qui est du test antioxydant, le test par le DPPH a permis d’obtenir un meilleur IC50 de 4,77 mg/ml contre 0,61 mg/ml chez le standard. Aussi, pour ce qui est du test de FRAP on a obtenu des extraits qui présentent une activité supérieure au standard avec des valeurs de 2853,33mgEC/g et de 2805,94mgEC/g contre 2781,85 mgEC/g du standard. Et, pour ce qui est du test de Folin, bien que le standard a présenté un taux de 57,03±0,55mgEC/g, les extraits renferment néanmoins une bonne teneur en composés phénoliques avec un taux de 37,89±1,48mgEC/g pour l’extrait le plus riche en composés phénoliques. Enfin, pour ce qui est du test de l’alpha amylase, les extraits précisément aqueux, éthanolique et hydroéthanolique de chaque formulation ont présentés une meilleure activité inhibitrice que le standard. Ces résultats suggèrent que les extraits issus des champignons formulés présentent une activité antioxydante considérable et une bonne capacité à inhiber l’activité de l’alpha amylase. Mots clés : Diabète, champignons pleurotes, antioxydants et alpha amylase


Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
N Orhan ◽  
M Aslan ◽  
S Hoşbaş ◽  
D Deliorman Orhan

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
L Moldovan ◽  
O Craciunescu ◽  
L Toma ◽  
A Gaspar ◽  
D Constantin

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
G Costa ◽  
S González-Manzano ◽  
A González-Paramás ◽  
C Santos-Buelga ◽  
IV Figueiredo ◽  
...  

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