In vitro alpha-amylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats

Objective: This study was aimed to evaluate the antioxidant potential of sinapic acid in both in vitro and in vivo. Recently, we have reported that oral administration of sinapic acid (3,5-dimethoxy 4-hydroxycinnamic acid) an active phyto ingredient widely distributed in rye, mustard, berries, and vegetables has been shown to ameliorate hyperglycemia.Methods: Experimental Type 2 diabetes was induced in male Wistar rats by feeding high-fat diet to induce insulin resistance followed by intraperitoneal administration of a single low dose streptozotocin (35 mg/kg body weight [bw]). Sinapic acid was administered orally at a concentration of 25 mg/kg bw/rat/day for 30 days, and its efficacy was compared with metformin. In vitro, antioxidant scavenging properties of sinapic acid were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), superoxide, and nitric oxide (NO) assay.Results: Sinapic acid treatment showed a significant decline in the levels of lipid peroxides, hydroperoxides and protein carbonyls in the plasma and vital tissues of diabetic rats. The treatment also improved the antioxidant status in diabetic rats indicating the antioxidant potential of sinapic acid. In addition, the results of DPPH, ABTS, superoxide, and NO radical scavenging assays substantiate the free radical scavenging efficacy of sinapic acid.Conclusion: The results of this study evidenced that sinapic acid possess significant antioxidant properties which in turn may be responsible for its antidiabetic properties.

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Persea americana Leaf Ethyl Acetate Extract Phytochemical, In-vitro Antioxidant and In-vivo Potentials to Mitigate Oxidative Stress in Alloxan-induced Hyperglycaemic Rats

Asian Plant Research Journal ◽

10.9734/aprj/2019/v2i230041 ◽

2019 ◽

pp. 1-11

Author(s):

J. A. Mashi ◽

A. M. Sa’id ◽

R. I. Idris ◽

I. Aminu ◽

A. A. Muhammad ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Diabetic Rats ◽

Persea Americana ◽

Albino Rats ◽

Standard Drug

The purpose of this study was to investigate the in-vivo and in-vitro potentials of ethyl acetate extract of P. americana leaf in alloxan-induced diabetic rats. Quantitative phytochemicals analyzed includes; flavonoids, saponins, tannins, alkaloids and phenolics. Measurement of antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl, total antioxidant capacity, hydroxyl radical, hydrogen peroxide, superoxide radical and ferric reducing activity of the extract was carried out. Hyperglycemia was induced by intraperitoneal injection of alloxan monohydrate to albino rats. In-vivo anti-oxidant potentials of the extract were evaluated by measuring liver homogenate activity of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and malondyaldehyde in alloxan-induced diabetic rats administered with the extract. A total of 30 Albino rats were used for this experiment and they were divided into six groups of 5 rats each. Group A; normal control, Group B; diabetic control, Groups C-E; experimental groups administered with different doses (100, 200 and 400 mg/kg body weight respectively); of the extract and Group F; glucophage (84 mg/kg body weight, standard drug) for 4 weeks. This study was conducted in the Department of Biochemistry, Bayero University, Kano, in August, 2018. Data was analyzed using one-way ANOVA with P=.05 value considered as significant. Results of the quantitative phytochemical investigation shows that the extract is rich in phenolics (184.1±0.6), flavonoids (115.8±2.1), alkaloids (41.5±1.8), with least concentration of tannis (21.2±0.8) and saponins (15.2±2.3). The extract exhibited high radical scavenging activity against synthetic free radicals (DPPH), reactive oxygen species (peroxide, superoxide and hydroxyl acid) and high ability to reduce Fe3+ to Fe2+ (FRAP). The activities of antioxidant enzymes of the treated rats were increased significantly (P=.05) while the level malondyaldehyde was significantly decreased (P=.05) in the treated groups. Ethyl acetate leaf extract of Persea americana contains phytochemical substances which improved antioxidant status and can be use as herbal therapy for the management of oxidative stress induced by diabetes mellitus and associated complications.

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In vitro antioxidant potential and in vivo effects of Schinus terebinthifolia Raddi leaf extract in diabetic rats and determination of chemical composition by HPLC-ESI-MS/MS

Natural Product Research ◽

10.1080/14786419.2018.1425848 ◽

2018 ◽

Vol 33 (11) ◽

pp. 1655-1658 ◽

Cited By ~ 2

Author(s):

Camila Cristina Iwanaga ◽

Lilian dos Anjos Oliveira Ferreira ◽

Karine Zanoli Bernuci ◽

Carla Maria Mariano Fernandez ◽

Fabiana Brusco Lorenzetti ◽

...

Keyword(s):

Chemical Composition ◽

Leaf Extract ◽

Diabetic Rats ◽

Antioxidant Potential ◽

Esi Ms ◽

In Vivo Effects

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Is There a Correlation Between In Vitro Antioxidant Potential and In Vivo Effect of Carvacryl Acetate Against Oxidative Stress in Mice Hippocampus?

Neurochemical Research ◽

10.1007/s11064-014-1267-5 ◽

2014 ◽

Vol 39 (4) ◽

pp. 758-769 ◽

Cited By ~ 13

Author(s):

Lúcio Fernandes Pires ◽

Luciana Muratori Costa ◽

Antonia Amanda Cardoso de Almeida ◽

Oskar Almeida Silva ◽

Gilberto Santos Cerqueira ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Potential

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In vitroandin vivoevaluation of an exopolysaccharide produced byLactobacillus helveticusKLDS1.8701 for the alleviative effect on oxidative stress

Food & Function ◽

10.1039/c8fo01920g ◽

2019 ◽

Vol 10 (3) ◽

pp. 1707-1717 ◽

Cited By ~ 10

Author(s):

Bailiang Li ◽

Peng Du ◽

Etareri Evivie Smith ◽

Song Wang ◽

Yuehua Jiao ◽

...

Keyword(s):

Oxidative Stress ◽

Lactobacillus Helveticus ◽

Antioxidant Potential

We systematically investigated thein vitroandin vivoantioxidant potential of EPS produced byLactobacillus helveticusKLDS1.8701.

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Antioxidant Potential of Psychotropic Drugs: From Clinical Evidence to In Vitro and In Vivo Assessment and toward a New Challenge for in Silico Molecular Design

Antioxidants ◽

10.3390/antiox9080714 ◽

2020 ◽

Vol 9 (8) ◽

pp. 714 ◽

Cited By ~ 3

Author(s):

Giovanni Ribaudo ◽

Marco Bortoli ◽

Chiara Pavan ◽

Giuseppe Zagotto ◽

Laura Orian

Keyword(s):

Oxidative Stress ◽

In Silico ◽

Rational Design ◽

Molecular Design ◽

Antioxidant Potential ◽

Oxidative Stress Biomarkers ◽

Systematic Analysis ◽

Antioxidant Supplements

Due to high oxygen consumption, the brain is particularly vulnerable to oxidative stress, which is considered an important element in the etiopathogenesis of several mental disorders, including schizophrenia, depression and dependencies. Despite the fact that it is not established yet whether oxidative stress is a cause or a consequence of clinic manifestations, the intake of antioxidant supplements in combination with the psychotropic therapy constitutes a valuable solution in patients’ treatment. Anyway, some drugs possess antioxidant capacity themselves and this aspect is discussed in this review, focusing on antipsychotics and antidepressants. In the context of a collection of clinical observations, in vitro and in vivo results are critically reported, often highlighting controversial aspects. Finally, a new challenge is discussed, i.e., the possibility of assessing in silico the antioxidant potential of these drugs, exploiting computational chemistry methodologies and machine learning. Despite the physiological environment being incredibly complex and the detection of meaningful oxidative stress biomarkers being all but an easy task, a rigorous and systematic analysis of the structural and reactivity properties of antioxidant drugs seems to be a promising route to better interpret therapeutic outcomes and provide elements for the rational design of novel drugs.

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In vivo correlation of olive leaves extract on some oxidative stress markers in streptozotocin-induced diabetes mellitus in rats

Grasas y Aceites ◽

10.3989/gya.1104172 ◽

2018 ◽

Vol 69 (1) ◽

pp. 243 ◽

Cited By ~ 2

Author(s):

A. M.R. Afify ◽

H. S. El-Beltagi ◽

S. A. Fayed ◽

A. E. El-Ansary

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Diabetic Control ◽

Diabetic Rats ◽

Adult Rats ◽

Male Adult ◽

Stress Markers ◽

Streptozotocin Induced Diabetes

Diabetes mellitus type two (T2DM) is one of the most extensive diseases in the world. Herbal therapy remains a possible adjunct therapy to sustain better glycemic control and reduce complications arising from diabetes. In order to evaluate the curative impacts of olive leaf extract (OLE) on streptozotocin (STZ)-induced diabetic rats, twenty-four Wistar male adult rats were divided into four equal groups; control, diabetic control (45 mg/kg STZ), normal rats treated with OLE (17.8 mg/kg b.wt.), and diabetic rats treated with OLE (45 mg/kg STZ + 17.8 mg/kg b.wt.). The OLE extract was investigated for in vitro antioxidant activity using the DPPH• assay. The phenolic, tannin, and flavonoid contents were determined. The activity of GPX, SOD, and GSH in RBC lysate, CAT in plasma and MDA in serum were measured. The OLE prevented the decrease in GSH and kept MDA around the normal range in the treated diabetic rats. The current study suggests that OLE might be used safely to ameliorate T2DM and its accompanying oxidative stress.

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Core structure of flavonoids precursor as an antihyperglycemic and antihyperlipidemic agent: an in vivo study in rats.

Acta Biochimica Polonica ◽

10.18388/abp.2010_2443 ◽

2010 ◽

Vol 57 (4) ◽

Cited By ~ 24

Author(s):

Mahmoud Najafian ◽

Azadeh Ebrahim-Habibi ◽

Parichehreh Yaghmaei ◽

Kazem Parivar ◽

Bagher Larijani

Keyword(s):

Beneficial Effect ◽

Therapeutic Potential ◽

Urine Volume ◽

Diabetic Rats ◽

Core Structure ◽

Alpha Amylase ◽

Glucose Levels ◽

Blood Glucose Levels

trans-Chalcone is the core structure of naringenin chalcone, located halfway in the biosynthesis pathway of flavonoids. Flavonoids have been reported as mammalian alpha-amylase inhibitors, a property which could be useful in the management of postprandial hyperglycemia in diabetes and related disorders. As a mammalian alpha-amylase inhibitor in vitro, the putative beneficial effect of trans-chalcone on diabetes was tested in a streptozotocin-induced rat model of diabetes type 1, and the results analyzed with commonly used statistical methods. Significant reduction of blood glucose levels and beneficial effect on dyslipidemia were observed in diabetic rats, as well as reduction of disturbing consequences of diabetes such as high urine volume and water intake. trans-chalcone was observed to have a weight loss-inductive effect, alongside with a reduction in food intake, which is suggestive of a therapeutic potential of this compound in overweight and obese patients.

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Biomarkers and Utility of the Antioxidant Potential of Probiotic Lactobacilli and Bifidobacteria as Representatives of the Human Gut Microbiota

Biomedicines ◽

10.3390/biomedicines9101340 ◽

2021 ◽

Vol 9 (10) ◽

pp. 1340

Author(s):

Olga V. Averina ◽

Elena U. Poluektova ◽

Mariya V. Marsova ◽

Valery N. Danilenko

Keyword(s):

Oxidative Stress ◽

Gut Microbiota ◽

Antioxidant Properties ◽

Natural Antioxidants ◽

Antioxidant Potential ◽

Diagnostic Systems ◽

Human Gut ◽

Human Gut Microbiota

Lactobacilli and bifidobacteria are an important part of human gut microbiota. Among numerous benefits, their antioxidant properties are attracting more and more attention. Multiple in vivo and in vitro studies have demonstrated that lactobacilli and bifidobacteria, along with their cellular components, possess excellent antioxidant capacity, which provides a certain degree of protection to the human body against diseases associated with oxidative stress. Recently, lactobacilli and bifidobacteria have begun to be considered as a new source of natural antioxidants. This review summarizes the current state of research on various antioxidant properties of lactobacilli and bifidobacteria. Special emphasis is given to the mechanisms of antioxidant activity of these bacteria in the human gut microbiota, which involve bacterial cell components and metabolites. This review is also dedicated to the genes involved in the antioxidant properties of lactobacilli and bifidobacteria strains as indicators of their antioxidant potential in human gut microbiota. Identification of the antioxidant biomarkers of the gut microbiota is of great importance both for creating diagnostic systems for assessing oxidative stress and for choosing strategies aimed at restoring the normal functioning of the microbiota and, through it, restoring human health. In this review, the practical application of probiotic strains with proven antioxidant properties to prevent oxidative stress is also considered.

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