scholarly journals Histological evaluation of mineral trioxide aggregate and enamel matrix derivative combination in direct pulp capping: An in vivo study

2016 ◽  
Vol 19 (6) ◽  
pp. 536 ◽  
Author(s):  
IndiraPriyadarshini Bollu ◽  
LDeepa Velagula ◽  
Nagesh Bolla ◽  
KKiran Kumar ◽  
Archana Hari ◽  
...  
Keyword(s):  
Mineral Trioxide Aggregate ◽  
In Vivo Study ◽  
Histological Evaluation ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Matrix Derivative

Direct Pulp Capping in Primary Molars with Enamel Matrix Derivative: Report of a Case

2009 ◽  
Vol 34 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Arturo Garrocho-Rangel ◽  
Hector Flores ◽  
Daniel Silva-Herzog ◽  
Raul Rosales-Ibañez ◽  
Amaury Pozos-Guillen
Keyword(s):  
Root Resorption ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Primary Molar ◽  
Radiographic Findings ◽  
Pulp Capping ◽  
External Root Resorption ◽  
Matrix Derivative

Enamel Matrix Derivative (EMD) is a rich amelogenin and amelin biomaterial that has been demonstrated to induce a reparative process similar to normal odontogenesis when placed in contact with pulp tissue. However, its effects in pulp capping on primary teeth has not been previously reported. The aim of the present case report is to present the favorable clinical and radiographic findings of a primary molar treated with direct pulp capping (DPC) and using EMD as capping material in a 6-year-old girl. Results: After 12 months, there was no sign or symptom indicative of treatment failure, such as pain, gingival swelling, sinus tract, sensitivity to percussion or palpation, abnormal mobility, widening of periodontal space, internal or external root resorption, or supporting bone or furcal area radiolucencies.

scholarly journals The Enhancing Effects of Amelogenin Exon 5-Encoded Peptide from Enamel Matrix Derivative on Odontoblast-Like KN-3 Cells

2018 ◽  
Vol 8 (10) ◽  
pp. 1890 ◽  
Author(s):  
Hirohito Kato ◽  
Yoichiro Taguchi ◽  
Kazutaka Imai ◽  
Yaru Ruan ◽  
Yu-Wei Tsai ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Biological Function ◽  
Calcium Deposition ◽  
Enamel Matrix Derivative ◽  
Nodule Formation ◽  
Enamel Matrix ◽  
Pulp Capping ◽  
Odontoblastic Differentiation ◽  
Dental Pulp Capping ◽  
Matrix Derivative

Enamel matrix derivative (EMD) is applied for periodontal therapy. We created a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. However, it is unknown whether SP affects odontoblastic differentiation. In this study, we tested the effects of SP in the odontoblast-like cells, KN-3 cells. KN-3 cells were cultured with SP (0 to 1000 ng/mL) and then cultured for 3, 8, 24, or 48 h in order to determine the effects of SP on cell proliferation and detect its optimum concentration. KN-3 cells were treated with SP in odontogenic differentiation medium cultured for 3 or 7 days. Odontogenic markers were measured by the detection of alkaline phosphatase (ALP) activity and dentin sialo phosphoprotein (DSPP) expression, the calcified nodule formation, and calcium deposition. The addition of SP significantly promoted cell proliferation at 100 ng/mL, generating the greatest change in cell proliferation. SP also showed increased odontogenic expression markers and mineralization. These results suggest that SP, derived from EMD, could have potential for application in dental pulp capping.

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