The Enhancing Effects of Amelogenin Exon 5-Encoded Peptide from Enamel Matrix Derivative on Odontoblast-Like KN-3 Cells

Enamel matrix derivative (EMD) is applied for periodontal therapy. We created a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. However, it is unknown whether SP affects odontoblastic differentiation. In this study, we tested the effects of SP in the odontoblast-like cells, KN-3 cells. KN-3 cells were cultured with SP (0 to 1000 ng/mL) and then cultured for 3, 8, 24, or 48 h in order to determine the effects of SP on cell proliferation and detect its optimum concentration. KN-3 cells were treated with SP in odontogenic differentiation medium cultured for 3 or 7 days. Odontogenic markers were measured by the detection of alkaline phosphatase (ALP) activity and dentin sialo phosphoprotein (DSPP) expression, the calcified nodule formation, and calcium deposition. The addition of SP significantly promoted cell proliferation at 100 ng/mL, generating the greatest change in cell proliferation. SP also showed increased odontogenic expression markers and mineralization. These results suggest that SP, derived from EMD, could have potential for application in dental pulp capping.

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The Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative Enhances in Odontoblast like Cells, KN-3 Cells

10.20944/preprints201808.0527.v1 ◽

2018 ◽

Author(s):

Hirohito Kato ◽

Yoichiro Taguchi ◽

Kazuya Tominaga ◽

Kazutaka Imai ◽

Yaru Ruan ◽

...

Keyword(s):

Cell Proliferation ◽

Biological Function ◽

Calcium Deposition ◽

Enamel Matrix Derivative ◽

Enamel Matrix ◽

Dentin Sialophosphoprotein ◽

Odontogenic Differentiation ◽

Dental Pulp Capping ◽

Periodontal Tissue Regeneration ◽

Matrix Derivative

Enamel matrix derivative (EMD) is used for periodontal tissue regeneration therapy. We designed a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. However, it is unknown whether SP affects odontoblastic differentiation. In the present study, we investigated the effects of SP in odontoblast-like cells, KN-3 cells. KN-3 cells were treated with SP (0, 1, 10, 100, or 1000 ng/mL) and then cultured for 3, 8, 24, or 48 hours, in order to determine the effects of SP on cell proliferation and detect its optimum concentration. To investigate the effect of SP on odontogenic differentiation, KN-3 cells were treated with SP in odontogenic differentiation medium cultured for 3 or 7 days. Odontogenic differentiation was performed by measuring alkaline phosphatase (ALP) activity, the mRNA expression of dentin sialophosphoprotein (DSPP), the formation of calcified nodules, and calcium deposition into the extracellular matrix. The addition of SP significantly promoted KN-3 cell proliferation; a concentration of 100 ng/ml generated the greatest change in cell proliferation. SP also showed increased expression of markers of odontogenic differentiation and mineralization. These results suggest that SP, derived from EMD, could be a potential for applicate to the dental pulp capping.

Download Full-text