Modification of dentin surface to enamel-like structure: A potential strategy for improving dentin bonding durability, desensitizing and self-repairing

Purpose: The purpose of this study was to evaluate the immediate bond strength of two sixth generation and two seventh generation dentin bonding agents to superficial dentin. Specimens were prepared to exposed superficial dentin and either Clearfil SE, Clearfil S3, Adper Prompt-l-pop of G-bond was applied over the dentin surface and light cured. Then composite resin was applied to the treated surface and light-cured in two increments. Specimens were tested 15 minutes after they were made for shear bond strength at 0.01 cm/min. Clearfil SE had statistically higher bond strength than the other three adhesives evaluated (42.9 MPa). There was no statistical difference among Clearfil S3, Adper Prompt-l-pop and G-Bond. The dentin adhesive with an application of an acidic primer before the application of the adhesive showed better immediate bond strength.

Download Full-text

Effect of Cavity Cleanser With Long-Term Antibacterial and Anti-Proteolytic Activities on Resin–Dentin Bond Stability

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.784153 ◽

2021 ◽

Vol 11 ◽

Author(s):

Yaping Gou ◽

Wei Jin ◽

Yanning He ◽

Yu Luo ◽

Ruirui Si ◽

...

Keyword(s):

Binding Capacity ◽

Oral Microbiome ◽

Control Group ◽

Secondary Caries ◽

Dentin Bonding ◽

Dentin Surface ◽

Proteolytic Activities ◽

Hybrid Layers ◽

Bond Durability

ObjectiveSecondary caries caused by oral microbiome dysbiosis and hybrid layer degradation are two important contributors to the poor resin–dentin bond durability. Cavity cleansers with long-term antimicrobial and anti-proteolytic activities are in demand for eliminating bacteria-induced secondary caries and preventing hybrid layers from degradation. The objectives of the present study were to examine the long-term antimicrobial effect and anti-proteolytic potential of poly(amidoamine) dendrimers with amino terminal groups (PAMAM-NH2) cavity cleanser.MethodsAdsorption tests by attenuated total reflectance–infrared (ATR-IR) spectroscopy and confocal laser scanning microscopy (CLSM) were first performed to evaluate whether the PAMAM-NH2 cavity cleanser had binding capacity to dentin surface to fulfill its relatively long-term antimicrobial and anti-proteolytic effects. For antibacterial testing, Streptococcus mutans, Actinomyces naeslundii, and Enterococcus faecalis were grown on dentin surfaces, prior to the application of cavity cleanser. Colony-forming unit (CFU) counts and live/dead bacterial staining were performed to assess antibacterial effects. Gelatinolytic activity within the hybrid layers was directly detected by in situ zymography. Adhesive permeability of bonded interface and microtensile bond strength were employed to assess whether the PAMAM-NH2 cavity cleanser adversely affected resin–dentin bonding. Finally, the cytotoxicity of PAMAM-NH2 was evaluated by the Cell Counting Kit-8 (CCK-8) assay.ResultsAdsorption tests demonstrated that the binding capacity of PAMAM-NH2 on dentin surface was much stronger than that of 2% chlorhexidine (CHX) because its binding was strong enough to resist phosphate-buffered saline (PBS) washing. Antibacterial testing indicated that PAMAM-NH2 significantly inhibited bacteria grown on the dentin discs as compared with the control group (p < 0.05), which was comparable with the antibacterial activity of 2% CHX (p > 0.05). Hybrid layers conditioned with PAMAM-NH2 showed significant decrease in gelatin activity as compared with the control group. Furthermore, PAMAM-NH2 pretreatment did not adversely affect resin–dentin bonding because it did not decrease adhesive permeability and microtensile strength. CCK-8 assay showed that PAMAM-NH2 had low cytotoxicity on human dental pulp cells (HDPCs) and L929.ConclusionsPAMAM-NH2 cavity cleanser developed in this study could provide simultaneous long-term antimicrobial and anti-proteolytic activities for eliminating secondary caries that result from a dysbiosis in the oral microbiome and for preventing hybrid layers from degradation due to its good binding capacity to dentin collagen matrix, which are crucial for the maintenance of resin–dentin bond durability.

Download Full-text

Effects of resveratrol/ethanol pretreatment on dentin bonding durability

Materials Science and Engineering C ◽

10.1016/j.msec.2020.111000 ◽

2020 ◽

Vol 114 ◽

pp. 111000 ◽

Cited By ~ 1

Author(s):

Wenan Peng ◽

Luyao Yi ◽

Ziming Wang ◽

Hongye Yang ◽

Cui Huang

Keyword(s):

Dentin Bonding ◽

Bonding Durability ◽

Ethanol Pretreatment

Download Full-text

A niobophosphate bioactive glass suspension for rewetting dentin: Effect on antibacterial activity, pH and resin-dentin bonding durability

International Journal of Adhesion and Adhesives ◽

10.1016/j.ijadhadh.2018.03.004 ◽

2018 ◽

Vol 84 ◽

pp. 178-183 ◽

Cited By ~ 3

Author(s):

José Bauer ◽

Allana Silva e Silva ◽

Edilausson Moreno Carvalho ◽

Ceci Nunes Carvalho ◽

Ricardo Marins Carvalho ◽

...

Keyword(s):

Antibacterial Activity ◽

Bioactive Glass ◽

Dentin Bonding ◽

Bonding Durability ◽

Glass Suspension

Download Full-text

Degree of Conversion Contributes to Dentin Bonding Durability of Contemporary Universal Adhesives

Operative Dentistry ◽

10.2341/19-165-l ◽

2020 ◽

Vol 45 (5) ◽

pp. 556-566 ◽

Cited By ~ 3

Author(s):

A Tichy ◽

K Hosaka ◽

A Abdou ◽

M Nakajima ◽

J Tagami

Keyword(s):

Bond Strength ◽

Resin Composite ◽

Degree Of Conversion ◽

Significance Level ◽

Dentin Bonding ◽

Light Curing ◽

Bonding Durability ◽

Universal Adhesives ◽

High Degree

Clinical Relevance The degree of conversion of contemporary universal adhesives positively correlates with the bond strength to dentin. The correlation is more marked after thermocycling, suggesting that a high degree of conversion is required for long-term dentin bonding durability. SUMMARY Purpose: The objectives of this study were to evaluate the micro-tensile bond strength (μTBS) of five contemporary universal adhesives to dentin after 24 hours and thermocycling (TC), to measure their degrees of conversion (DC) and to test the correlation between μTBS and DC. Methods and Materials: Four commercially available universal adhesives, Prime&Bond universal (PBU), Ecosite Bond (EB), G-Premio Bond (GPB), and Clearfil Universal Bond Quick (UBQ), and one experimental adhesive, UBQ without an amide monomer (UBQ-A), were used in this study. For the μTBS test, midcoronal dentin of 50 human molars was exposed, ground using 600-grit SiC paper, and the adhesives were applied according to the manufacturers’ instructions. After resin-composite buildup and 24-hour water storage, one-half of the specimens were subjected to 15,000 thermal cycles. The specimens were sectioned into beams and stressed in tension at a crosshead speed of 1 mm/min until failure. The DC of adhesives applied to dentin was evaluated using attenuated total reflectance Fourier-transform infrared spectroscopy immediately after light-curing. All data were statistically analyzed at a significance level of 0.05. Results: The highest μTBSs were obtained with UBQ, UBQ-A, and PBU, which were not significantly different from each other both after 24 hours and TC. The μTBS of GPB was lower compared with the aforementioned adhesives, but significantly only after TC, and the lowest μTBSs were obtained with EB. TC did not affect the μTBSs of UBQ, UBQ-A, and PBU significantly, but a significant decrease was observed with GPB and EB. The highest DC was obtained with PBU and UBQ, followed by 2-hydroxyethyl methacrylate–rich adhesives UBQ-A and EB, which exhibited significantly lower DCs. The DC of GPB could not be determined because the reference peak at 1608 cm−1 was not detected in its spectra. A significant positive correlation was shown between μTBS and DC after 24 hours (r=0.716) and TC (r=0.856). Conclusion: μTBS and DC were positively correlated, more markedly after TC, which suggests that DC may be an important factor for bond durability.

Download Full-text

Effect of Chitosan pretreatment of the demineralized dentin surface on the durability of resin dentin bonding. An in vitro study

Egyptian Dental Journal ◽

10.21608/edj.2019.72879 ◽

2019 ◽

Vol 65 (2) ◽

pp. 1697-1702

Author(s):

Mostafa Ata

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Dentin Bonding ◽

Dentin Surface ◽

Demineralized Dentin

Download Full-text

Marginal Adaptation of Class V Restorations with Current- Generation Dentin-Bonding Agents: Effect of Different Dentin Surface Treatments

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1538 ◽

2014 ◽

Vol 15 (3) ◽

pp. 331-335

Author(s):

Marcelo Ferrarezi de Andrade ◽

Alvaro Henrique Borges ◽

Matheus Coelho Bandéca ◽

José Roberto Cury Saad ◽

Edson Alves de Campos ◽

...

Keyword(s):

Mechanical Load ◽

Surface Treatments ◽

Wilcoxon Test ◽

Marginal Adaptation ◽

Class V ◽

Current Generation ◽

Bonding Agents ◽

Dentin Bonding ◽

Dentin Surface ◽

Dentin Bonding Agents

Abstract This study sought to assess the use of chlorhexidine with several excipients as a dentin surface treatment and its effect on marginal adaptation of class V restorations with currentgeneration dentin bonding agents. A total of 120 human third molars were selected and allocated into 12 groups, with standardized buccal class V restorations randomly divided into preconditioned dentin rinsed with: water; water + chlorhexidine; ethanol; or ethanol + chlorhexidine. After rinsing of dentin (previously conditioned with 35% phosphoric acid) with the test solutions, the Adper single bond 2, prime and bond 2.1, and Excite bonding systems were applied randomly. Restorations were performed with Filtek™ Z350 XT composite resin. The resulting specimens were subjected to thermal and mechanical load cycling. Quantitative analysis of marginal adaptation was performed on epoxy replicas by means of scanning electron microscopy. Results were assessed by means of the Kruskal- Wallis test (percentages of continuous margins) and Wilcoxon test (differences between percentages of continuous margins before and after thermal cycling and mechanical loading), at a significance level of p < 0.05. Outcomes in the chlorhexidinetreated groups were not superior to those obtained with other treatments. How to cite this article Cavalheiro JP, Tonetto MR, Borges ÁH, Bandéca MC, dos Santos RSS, de Campos EA, Saad JRC, Dantas AAR, de Andrade MF. Marginal Adaptation of Class V Restorations with Current-Generation Dentin-Bonding Agents: Effect of Different Dentin Surface Treatments. J Contemp Dent Pract 2014;15(3):331-335.

Download Full-text