scholarly journals Manual versus Automated Rodent Behavioral Assessment: Comparing Efficacy and Ease of Bederson and Garcia Neurological Deficit Scores to an Open Field Video-Tracking System

2014 ◽  
Vol 6 ◽  
pp. JCNSD.S13194 ◽  
Author(s):  
Fiona A. Desland ◽  
Aqeela Afzal ◽  
Zuha Warraich ◽  
J Mocco
Keyword(s):  
Open Field ◽  
Neurological Deficit ◽  
Tracking System ◽  
Behavioral Assessment ◽  
Cohort Analysis ◽  
Video Tracking ◽  
Field Analysis ◽  
Post Stroke ◽  
Increased Sensitivity ◽  
Video Tracking System

Animal models of stroke have been crucial in advancing our understanding of the pathophysiology of cerebral ischemia. Currently, the standards for determining neurological deficit in rodents are the Bederson and Garcia scales, manual assessments scoring animals based on parameters ranked on a narrow scale of severity. Automated open field analysis of a live-video tracking system that analyzes animal behavior may provide a more sensitive test. Results obtained from the manual Bederson and Garcia scales did not show significant differences between pre- and post-stroke animals in a small cohort. When using the same cohort, however, post-stroke data obtained from automated open field analysis showed significant differences in several parameters. Furthermore, large cohort analysis also demonstrated increased sensitivity with automated open field analysis versus the Bederson and Garcia scales. These early data indicate use of automated open field analysis software may provide a more sensitive assessment when compared to traditional Bederson and Garcia scales.

Abstract T P200: CD13 is an Immune Regulator in the CNS Following Ischemic Stroke

Stroke ◽  
2014 ◽  
Vol 45 (suppl_1) ◽  
Author(s):  
Sami Tarabishy ◽  
Anthony Patrizz ◽  
Nikolas S Mancini ◽  
Linda Shapiro ◽  
Louise D McCullough
Keyword(s):  
Immune Response ◽  
Ischemic Stroke ◽  
Open Field ◽  
Neurological Deficit ◽  
Focal Cerebral Ischemia ◽  
Brain Slices ◽  
Motor Dysfunction ◽  
Field Analysis ◽  
Cresyl Violet ◽  
Post Stroke

Background: Focal cerebral ischemia results in marked inflammatory response initiated by microglia and astrocyte activation, upregulation of pro-inflammatory cytokines, and disruption of the blood brain barrier leading to invasion of peripheral immune cells. CD13 (Aminopeptidase N), a 150 kD type II transmembrane metalloprotease highly expressed on myeloid cells, has recently been shown to possess several non-enzymatic functions involving the regulation of processes related to inflammation including antigen presentation and cell trafficking into sites of inflammation. We hypothesized that deletion of CD13 would alter the innate immune response after stroke leading to smaller infarcts and neuroprotection. Methods: C57BL/6 WT and CD13 KO male mice were subjected to transient focal ischemia by middle cerebral artery occlusion for 90 minutes. Motor and sensory deficits were assessed using the Benderson neurological deficit score and open field analysis. Mice were sacrificed at 48hrs and brain slices stained with Cresyl Violet for infarct analysis. Immunohistochemistry was performed on fresh frozen slices to visualize microglial activation. Results: There was significant reduction in total hemispheric and cortical infarct in KO compared to WT at 48 hours post stroke (Hemispheric 50.8 ± 2.0 WT vs. 40.7 ± 3.8 KO p=.04, Cortical 56.5 ± 4.0 WT vs. 40.1 ± 5.8 KO p=.04, n=6-10). Behaviorally, CD13 KO mice performed better in the open field test than WT, displaying less motor dysfunction (p=.047). 48hrs post stroke. CD13 KO mice had lower neurological deficit scores (3 WT vs. 2 KO, IQR=0, U=0, n=6-10). CD13 mice had less mortality at all time-points. IHC revealed increased microglia activation in the infarct hemisphere of KO mice when compared to WT. Conclusions: CD13 KO mice had smaller infarct sizes and better acute behavioral outcomes than WT mice. Furthermore, initial IHC results displayed a phenotypically different immune response in KO animals. Flow cytometry is characterizing the differences in the immune response between the groups and microglial phenotypes. Further identifying and targeting of the non-enzymatic functions of CD13 in the CNS may lead to novel therapeutic clinical treatments to reduce morbidity and mortality from ischemic stroke.

A Real-Time Video Tracking System

1979 ◽  
Vol 18 (1) ◽  
pp. 180125
Author(s):  
A. L. Gilbert
Keyword(s):  
Real Time ◽  
Tracking System ◽  
Video Tracking ◽  
Video Tracking System

Novel approach to automatically classify rat social behavior using a video tracking system

2016 ◽  
Vol 268 ◽  
pp. 163-170 ◽  
Author(s):  
Suzanne M. Peters ◽  
Ilona J. Pinter ◽  
Helen H.J. Pothuizen ◽  
Raymond C. de Heer ◽  
Johanneke E. van der Harst ◽  
...  
Keyword(s):  
Social Behavior ◽  
Tracking System ◽  
Video Tracking ◽  
Novel Approach ◽  
Video Tracking System

scholarly journals An improved light dark box test by using a real-time video tracking system

2018 ◽  
Vol 69 (4) ◽  
pp. 371-384
Author(s):  
Sareesh Naduvil Narayanan ◽  
Raju Suresh Kumar
Keyword(s):  
Real Time ◽  
Tracking System ◽  
Video Tracking ◽  
Video Tracking System ◽  
Light Dark Box

Remote Person Authentication using a Super-telephoto Video Tracking System

2019 ◽  
Vol 2019 (0) ◽  
pp. 2P1-I08
Author(s):  
Hiroki ADACHI ◽  
Mitsuhiro INOKUCHI ◽  
Kohei SHIMASAKI ◽  
Mingjun JIANG ◽  
Takeshi TAKAKI ◽  
...  
Keyword(s):  
Tracking System ◽  
Video Tracking ◽  
Video Tracking System

scholarly journals GroupTracker: Video tracking system for multiple animals under severe occlusion

2015 ◽  
Vol 57 ◽  
pp. 39-45 ◽  
Author(s):  
Tsukasa Fukunaga ◽  
Shoko Kubota ◽  
Shoji Oda ◽  
Wataru Iwasaki
Keyword(s):  
Tracking System ◽  
Video Tracking ◽  
Video Tracking System

Repeated measures of adult normal walking using a video tracking system

1997 ◽  
Vol 6 (2) ◽  
pp. 147-162 ◽  
Author(s):  
Eric Growney ◽  
Dwight Meglan ◽  
Marjorie Johnson ◽  
Thomas Cahalan ◽  
Kai-Nan An
Keyword(s):  
Repeated Measures ◽  
Tracking System ◽  
Video Tracking ◽  
Normal Walking ◽  
Video Tracking System

Courtship song in Drosophila melanogaster: a differential effect on male–female locomotor activity

2004 ◽  
Vol 82 (8) ◽  
pp. 1258-1266 ◽  
Author(s):  
Solange Kowalski ◽  
Thierry Aubin ◽  
Jean-René Martin
Keyword(s):  
Drosophila Melanogaster ◽  
Locomotor Activity ◽  
Tracking System ◽  
Species Recognition ◽  
Courtship Song ◽  
Video Tracking ◽  
Activity Level ◽  
Sexual Stimulation ◽  
Video Tracking System ◽  
Accurate Quantification

The courtship song of male Drosophila melanogaster Meigen, 1830 is involved in species recognition and sexual stimulation. This signal is usually addressed to the female to reduce her locomotor activity, thereby facilitating copulation. However, no accurate quantification of her locomotion has been made. To examine the effect of courtship song on locomotor behaviour of both sexes, we used a video-tracking system that allowed for the quantification of two indicators of activity level: distance moved and movement duration. First, we showed that the broadcast of the courtship song alone produced no effect on female locomotion. Females reduced their locomotor activity only when acoustical stimulation was placed in a natural courtship context (i.e., in the presence of a male). This suggests that the sum of visual, tactile, acoustic, and chemical stimuli provided by the male may act together to trigger female receptivity. Second, our playback experiments showed a strong stimulating effect of courtship song (particularly of the pulse component) on the locomotor activity of isolated males, suggesting that this signal probably plays a role in male stimulation. Courtship song has an opposite effect on male/female locomotor activity in D. melanogaster.

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