The transfer of leaf rust resistance from Triticum turgidum ssp. dicoccoides to hexaploid wheat

1994 ◽  
Vol 74 (4) ◽  
pp. 671-673 ◽  
Author(s):  
P. L. Dyck
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Triticum Turgidum ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Accession 8404 of Triticum turgidum ssp. dicoccoides was shown to have excellent resistance to leaf rust. Genetic analysis of the F3 of 8404 and RL6089, a leaf rust susceptible durum, indicated that 8404 had three genes for leaf rust resistance. Two of these genes were transferred to hexaploid wheat (Thatcher) by a series of backcrosses. One of the genes transferred was the same as Lr33 (RL6057). The second gene, which gives a fleck reaction to avirulent P. recondita races, appears to be fully incorporated into the hexaploid where it segregated to fit a one-gene ratio. Backcross lines with this gene give excellent resistance to leaf rust, although race MBG is virulent to this gene. This may be a previously unidentified leaf rust resistance gene and should increase the genetic diversity available for wheat breeders. Key words:Triticum aestivum, wheat, Triticum turgidum ssp. dicoccoides, leaf rust resistance

Molecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat

2012 ◽  
Vol 31 (3) ◽  
pp. 743-747 ◽  
Author(s):  
B. B. Dholakia ◽  
A. V. Rajwade ◽  
P. Hosmani ◽  
R. R. Khan ◽  
S. Chavan ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

scholarly journals Molecular mapping of leaf rust resistance gene LrL224 in Chinese wheat cultivar L224-3

2018 ◽  
Vol 54 (No. 2) ◽  
pp. 65-70
Author(s):  
Y. Zhou ◽  
H. Li ◽  
P.-P. Zhang ◽  
L. Wang ◽  
Z.-F. Li
Keyword(s):  
Genetic Analysis ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Chinese Wheat Cultivar ◽  
Chinese Wheat

Leaf rust, caused by Puccinia triticina, is a major wheat disease worldwide. The chinese wheat cultivar L224-3 showed high resistance to most of P. triticina pathotypes in the seedling and adult stage. Identifying and mapping the leaf rust resistance gene(s) in L224-3 is very useful for breeding leaf rust resistant wheat cultivars. In the present study, the wheat cultivar L224-3 and thirty-six lines with known leaf rust resistance genes were inoculated with 15 pathotypes at the seedling stage for gene postulation. A total of 144 F<sub>2:3</sub> lines from the cross L224-3 × Zhengzhou 5389 were inoculated with the pathotype FHBQ for leaf rust genetic analysis at the seedling stage. A total of 1276 SSR (simple sequence repeat) markers and the STS (sequence tagged-site) marker ω-secali/Glu-B3 were used to test the parents, resistant and susceptible bulks. The polymorphic markers were used to genotype the F<sub>2:3</sub> populations. L224-3 was highly resistant to all Lr26 avirulent pathotypes, showing the presence of Lr26 in L224-3. The presence of Lr26 in L224-3 was also confirmed by the molecular marker ω-secalin/Glu-B3. Due to resistance to some Lr26 virulent pathotypes, L224-3 may carry another resistance gene. Based on the genetic analysis using the pathotype FHBQ with virulence to Lr26 the resistance of L224-3 was controlled by a single dominant gene, tentatively designated LrL224. Four SSR markers (barc8, gwm582, wmc419, and wmc694) and one STS marker (ω-secali/Glu-B3) on 1B were closely linked to LrL224. The two flanking SSR loci were barc8 and gwm582, with the genetic distances of 4.3 and 4.6 cM, respectively. LrL224 was located on 1BL, and it showed different seedling reactions with other genes on 1B. Therefore LrL224 is likely to be a new leaf rust resistance gene.

scholarly journals Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  
Keyword(s):  
Amino Acid ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Agronomic Traits ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.

scholarly journals Microsatellite markers for the Triticum timopheevi-derived leaf rust resistance gene Lr18 on wheat 5BL chromosome

2017 ◽  
Vol 67 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Ali Aliakbari Sadeghabad ◽  
Ali Dadkhodaie ◽  
Bahram Heidari ◽  
Hooman Razi ◽  
Reza Mostowfizadeh-Ghalamfarsa
Keyword(s):  
Microsatellite Markers ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Triticum Timopheevi
Sign in / Sign up

Export Citation Format

Share Document