scholarly journals Variability of Bean yellow mosaic virus isolates in the Czech Republic

2009 ◽  
Vol 53 (4) ◽  
pp. 277-280 ◽  
Author(s):  
D. Ganesh_Selvaraj ◽  
R. Pokorny ◽  
L. Holkova
Author(s):  
Ganesh Selvaraj Duraisamy ◽  
Radovan Pokorný

The occurrence of Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) Tobacco rattle virus (TRV) in gladiolus, iris, tulip and Iris yellow spot virus (IYSV) in iris was investigated by examining the plants by the means of serological techniques (ELISA). ELISA was applied to determine the presence of BYMV, CMV, TRV infections in both aerial and underground parts of gladiolus, iris, and tulip, and IYSV on the aerial parts of iris, respectively. 262 gladiolus plants were tested. 63.7% was infected by BYMV, 29.4 % by CMV, and 2.7 % by TRV. Out of 180 plants of iris, 1.1% was infected by BYMV, 6.7% by CMV, 2.8% by TRV, and 0% by IYSV. Out of 28 plants of tulip, 28.6% was infected by CMV, and 7.1% by TRV. ELISA proved to be a suitable method for detection of viruses in leaves of these ornamental plants, but it often failed to detect viruses in flowers and corms. A high transmission of BYMV by gladiolus cormlets was also found.


2012 ◽  
Vol 38 (No. 4) ◽  
pp. 125-130 ◽  
Author(s):  
J. Svoboda ◽  
J. Polák

The incidence of Zucchini yellow mosaic virus (ZYMV) was monitored in the south Moravian region of the Czech Republic during 1997–2001. Crops of gherkin, squash, zucchini and cucumbers were found infected with ZYMV, manifested by symptoms of severe stunting and yellowing with deformed leaves and fruits. Twenty to eighty percent of infected plants were recorded. Six isolates of ZYMV from four localities were differentiated on indicator plants; three of them were maintained as being typical for differences in pathogenicity. Overwintered weed species were tested for the presence of the virus. ZYMV was detected by ELISA in one plant of Tripleurospermum maritimum out of 46 tested, and in two plants of Stellaria media out of 29 tested in 2001. Such tests were repeated in 2002, and ZYMV was detected in three plants of T. maritimum out of 45 tested, in three plants of S. media out of 52, and in two plants of Trifolium repens out of 17 tested. The virus was successfully transmitted from T. maritimum, S. media and T. repens to indicator plants of Cucurbita pepo convar. giromontiina. Back-transmission of ZYMV was proved by ELISA, electron microscopy and symptoms. T. maritimum was found to be a new natural host of ZYMV.


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