Evaluation of cross-protection with an attenuated isolate of Bean yellow mosaic virus by differential detection of virus isolates using RT-PCR

2004 ◽  
Vol 70 (6) ◽  
pp. 359-362 ◽  
Author(s):  
Eiko Nakazono-Nagaoka ◽  
Chikako Sato ◽  
Yoshitaka Kosaka ◽  
Tomohide Natsuaki
Keyword(s):  
Mosaic Virus ◽  
Cross Protection ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Differential Detection ◽  
Rt Pcr ◽  
Attenuated Isolate ◽  
Virus Isolates

RT-PCR-RFLP analysis for evaluating cross protection by an attenuated isolate of Cucumber mosaic virus

2005 ◽  
Vol 71 (3) ◽  
pp. 243-246 ◽  
Author(s):  
Eiko Nakazono-Nagaoka ◽  
Masako Suzuki ◽  
Yoshitaka Kosaka ◽  
Tomohide Natsuaki
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rflp Analysis ◽  
Cross Protection ◽  
Rt Pcr ◽  
Pcr Rflp ◽  
Attenuated Isolate

scholarly journals Relationships of Legume Viruses in Australia II. Serological Relationships of Bean Yellow Mosaic Virus and Pea Mosaic Virus

1956 ◽  
Vol 9 (2) ◽  
pp. 231 ◽  
Author(s):  
DJ Goodchild
Keyword(s):  
Mosaic Virus ◽  
Cross Protection ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Legume Viruses

Antisera were prepared to one strain of boan yellow mosaie virus (B.Y.lVLV.) and two strains of pea mosaie virus (P.i\LV. strain 1 and P.lII.V. sLraill 2). By utilizing the aggluLinatioll reaetiol1, the serological activit,y of both straills of P.lVLV. is clemollst,rated for the first timo. The results of erose-agglutination experim81lt,s show the strains of P.M. V. and B.Y.M.V. to be serologically related. Cross-protection tests support tho hypothesis that Lhose are strains of one and the same virlls.

scholarly journals Occurrence and distribution of viruses infecting the bean in Serbia

2010 ◽  
Vol 62 (3) ◽  
pp. 595-601 ◽  
Author(s):  
Dragana Petrovic ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
Milka Vujakovic ◽  
Mirjana Vasic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Viral Infections ◽  
Alfalfa Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Pcr Method ◽  
Virus Isolates ◽  
Viral Isolates

This work describes the incidence and distribution of the most important bean viruses in Serbia: Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). The viral isolates were characterized serologically and biologically. BCMV was found in the largest number of plants (30.53%), followed by BCMNV (2.67%), CMV (5.34%), and AMV (3.41%), since BYMV was not determined. Mixed viral infections were found in several samples. The RT-PCR method was used to prove that the tested isolates belong to the BCMV, family Potyviridae and strains Russian and NL-3 D. Results obtained in this work will enable further studies of the genetic variability of bean virus isolates from Serbia. .

scholarly journals Cross-Protection Against Bean yellow mosaic virus (BYMV) and Clover yellow vein virus by Attenuated BYMV Isolate M11

2009 ◽  
Vol 99 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Eiko Nakazono-Nagaoka ◽  
Tsubasa Takahashi ◽  
Takumi Shimizu ◽  
Yoshitaka Kosaka ◽  
Tomohide Natsuaki ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Phylogenetic Trees ◽  
Amino Acid Sequences ◽  
Cross Protection ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Vein ◽  
Yellow Mosaic Virus ◽  
Challenge Virus ◽  
Clover Yellow Vein Virus

Attenuated isolate M11 of Bean yellow mosaic virus (BYMV), obtained after exposing BYMV-infected plants to low temperature, and its efficacy in cross-protecting against infection by BYMV isolates from gladiolus, broad bean (Vicia faba) and white clover (Trifolium repens) was assessed with western blotting and reverse transcription-polymerase chain reaction. The level of cross-protection varied depending on the challenge virus isolates. Cross-protection was complete against BYMV isolates from gladiolus, but incomplete against BYMV isolates from other hosts. M11 also partially cross-protected against an isolate of Clover yellow vein virus. A comparison of the nucleotide sequence of M11 and those of BYMV isolates from gladiolus and from other hosts showed higher homology among gladiolus isolates than the homology between gladiolus isolates and nongladiolus isolates. In the phylogenetic trees, constructed using the nucleotide sequences of an overall polyprotein of the genomes, five gladiolus isolates clustered together, completely separated from the three BYMV isolates from other hosts. A comparison of the amino acid sequences between M11 and its parental isolate IbG, and analysis of recombinant infectious clones between M11 and IbG revealed that an amino acid at position 314 was involved in the attenuation of BYMV.

Selection of an attenuated isolate of Bean yellow mosaic virus for protection of dwarf gentian plants from viral infection in the field

2004 ◽  
Vol 70 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Hiroyuki Uga ◽  
Yuki O. Kobayashi ◽  
Kyoji Hagiwara ◽  
Yohachiro Honda ◽  
Toshihiro Omura
Keyword(s):  
Viral Infection ◽  
Mosaic Virus ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Attenuated Isolate ◽  
Selection Of
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