scholarly journals Distribution, variabiality and overwintering of Zucchini yellow mosaic virus in the Czech Republic

2012 ◽  
Vol 38 (No. 4) ◽  
pp. 125-130 ◽  
Author(s):  
J. Svoboda ◽  
J. Polák

The incidence of Zucchini yellow mosaic virus (ZYMV) was monitored in the south Moravian region of the Czech Republic during 1997–2001. Crops of gherkin, squash, zucchini and cucumbers were found infected with ZYMV, manifested by symptoms of severe stunting and yellowing with deformed leaves and fruits. Twenty to eighty percent of infected plants were recorded. Six isolates of ZYMV from four localities were differentiated on indicator plants; three of them were maintained as being typical for differences in pathogenicity. Overwintered weed species were tested for the presence of the virus. ZYMV was detected by ELISA in one plant of Tripleurospermum maritimum out of 46 tested, and in two plants of Stellaria media out of 29 tested in 2001. Such tests were repeated in 2002, and ZYMV was detected in three plants of T. maritimum out of 45 tested, in three plants of S. media out of 52, and in two plants of Trifolium repens out of 17 tested. The virus was successfully transmitted from T. maritimum, S. media and T. repens to indicator plants of Cucurbita pepo convar. giromontiina. Back-transmission of ZYMV was proved by ELISA, electron microscopy and symptoms. T. maritimum was found to be a new natural host of ZYMV.

Author(s):  
Heba A. Mahfouze ◽  
Sherin A. Mahfouze ◽  
Mahmoud E. S. Ottai

Squash or cucurbit (Cucurbita pepo) is one of the famous and important vegetable plants in most world countries. The squash is infected by the critical Zucchini yellow mosaic virus (ZYMV) in Egypt. Though pesticides can protect the plants of pest infections, there are no effective compounds that can be applied as virucides. In this work, the effects of aqueous extracts from Jasmina montana and Artemisia herba-alba on ZYMV infection in the squash plants were examined. In addition, SDS-PAGE protein patterns and enzyme activities were evaluated and induction of resistance by plant elicitors against ZYMV was studied The results observed that using of the aqueous extracts from A. herba-alba or J. montana extracts prior to ZYMV inoculation recorded 100% inhibition of virus infection. Also, complete inhibition was obtained by treatment of A. herba-alba extract with the virus inoculum led to destroy the virus particles due to presence of the effective compounds (terpens, phenolics and the essential oils) in the plant extract. SDS-PAGE protein profiles and enzyme activities were studied in treated and untreated plants. Genomic DNA variation was studied using random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeat (ISSR) loci. The alterations in RAPD and ISSR assays of representative squash plants can be applied to comprehend of induced systemic resistance. These inducers stimulated resistance in the squash plants. Therefore, elicitors should be taken into consideration in the breeding programs for ZYMV control.


2010 ◽  
Vol 45 (Special Issue) ◽  
pp. S20-S26 ◽  
Author(s):  
J. Polák

Results of research aiming at monitoring of climate changes impact on plant pathogens distribution such as <i>Zucchini yellow mosaic virus</i> (ZYMV), quarantine <i>Plum pox virus</i> (PPV) and quarantine phytoplasma European stone fruit yellows (ESFY) are presented here. ZYMV has spread from Northern Italy across Austria up to Central Moravia and Bohemia. PPV has been continuously spreading from the lowlands of Central Bohemia and Moravia up to plains. Later, from the sixties and seventies of the last century, due to climate warming and human activities the virus quickly spread to uplands, foothills and mountains of the Czech Republic. Phytoplasma ESFY was spreading in a manner similar to ZYMV in the eighties of the twentieth century from Northern Italy and currently is affecting mainly apricot and peach trees in Southern Moravia.


2007 ◽  
Vol 33 (2) ◽  
pp. 192-194 ◽  
Author(s):  
Mônica Juliani Zavaglia Pereira ◽  
Angelo Aparecido Barbosa Sussel ◽  
Ricardo Ferreira da Silva ◽  
Odair José Kuhn ◽  
Fernanda Domingues ◽  
...  

Este trabalho teve por objetivo avaliar, em condições de casa de vegetação e de campo, os danos causados pelo PRSV-W e ZYMV em abobrinha-de-moita (Cucurbita pepo cv. Caserta). As plantas em casa de vegetação foram inoculadas com os vírus individualmente e em mistura aos 12 e 22 dias após emergência (DAE) e aos 5, 15 e 25 DAE no campo. Em casa de vegetação, as infecções com PRSV-W + ZYMV, PRSV-W e ZYMV, na primeira época de inoculação, ocasionaram reduções de área foliar de 39,6%, 36,8% e 12,1%, respectivamente. As massas fresca e seca também foram significativamente afetadas na primeira época de inoculação. No campo, as plantas com infecções individuais ou mistas dos potyvírus produziram frutos não comerciais em quantidades que variaram de 14 a 861 g/planta, dependendo da idade que foram inoculadas. As plantas tratadas com tampão fosfato aos 5, 15 e 25 DAE produziram em média 573 g, 937 g e 1172 g de frutos comerciais e 282 g, 221 g e 192 g de frutos não comerciais, respectivamente. A redução na massa fresca das plantas foi diretamente relacionada com a época de inoculação, com médias de 60,7% para aquelas inoculadas aos 5 DAE e de 22,7% para aquelas inoculadas aos 15 DAE. Na terceira época de inoculação não houve diferença significativa de massa fresca entre os tratamentos.


2014 ◽  
Vol 134 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Martin Pachner ◽  
Harry S. Paris ◽  
Johanna Winkler ◽  
Tamas Lelley

2010 ◽  
Vol 25 (3) ◽  
pp. 213-230
Author(s):  
Ana Vucurovic ◽  
Aleksandra Bulajic ◽  
Ivana Stankovic ◽  
Danijela Ristic ◽  
Janos Berenji ◽  
...  

Watermelon mosaic virus (WMV) is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group), as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELI


Author(s):  
Ganesh Selvaraj Duraisamy ◽  
Radovan Pokorný

The occurrence of Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) Tobacco rattle virus (TRV) in gladiolus, iris, tulip and Iris yellow spot virus (IYSV) in iris was investigated by examining the plants by the means of serological techniques (ELISA). ELISA was applied to determine the presence of BYMV, CMV, TRV infections in both aerial and underground parts of gladiolus, iris, and tulip, and IYSV on the aerial parts of iris, respectively. 262 gladiolus plants were tested. 63.7% was infected by BYMV, 29.4 % by CMV, and 2.7 % by TRV. Out of 180 plants of iris, 1.1% was infected by BYMV, 6.7% by CMV, 2.8% by TRV, and 0% by IYSV. Out of 28 plants of tulip, 28.6% was infected by CMV, and 7.1% by TRV. ELISA proved to be a suitable method for detection of viruses in leaves of these ornamental plants, but it often failed to detect viruses in flowers and corms. A high transmission of BYMV by gladiolus cormlets was also found.


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