Comparison of IgE-binding components between 2 house dust mites in adult allergic patients

Group 10 allergens (tropomyosins) have been assumed to be a major cause of cross-reactivity between house-dust mites (HDMs) and other invertebrates. Despite all of the published data regarding the epidemiology, percent IgE binding and level of sensitization in the population, the role of tropomyosin as a cross-reactive allergen in patients with multiple allergy syndrome still remains to be elucidated. Homology between amino acid sequences reported in allergen databases of selected invertebrate tropomyosins was determined with Der f 10 as the reference allergen. The 66.9 and 54.4% identities were found with selected crustacean and insect species, respectively, whereas only 20.4% identity was seen with mollusks. A similar analysis was performed using reported B-cell IgE-binding epitopes from Met e1 (shrimp allergen) and Bla g7 (cockroach allergen) with other invertebrate tropomyosins. The percent identity in linear sequences was higher than 35% in mites, crustaceans, and cockroaches. The polar and hydrophobic regions in these groups were highly conserved. These findings suggest that tropomyosin may be a major cause of covariation of sensitization between HDMs, crustaceans, and some species of insects and mollusks.

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IgE AND IgG antibody responses to Dermatophagoides pteronyssinus in dogs with demodicosis and atopic dermatitis

Bioscience Journal ◽

10.14393/bj-v36n6a2020-48029 ◽

2020 ◽

Vol 36 (6) ◽

Author(s):

Maria Cecília Oliveira ◽

Ana Cláudia Arantes Marquez Pajuaba ◽

José Eugênio Diniz Bastos ◽

José Roberto Mineo ◽

Fabiana Parreira Souza ◽

...

Keyword(s):

House Dust ◽

Skin Diseases ◽

Specific Ige ◽

Dermatophagoides Pteronyssinus ◽

House Dust Mites ◽

Dust Mites ◽

Ige Antibodies ◽

Ige Binding ◽

Healthy Dogs ◽

Demodex Mites

Canine demodicosis is a common inflammatory parasitic skin disease caused by Demodex mites. House dust mites, such as Dermatophagoides spp., play an important role in the pathogenesis of canine atopic dermatitis (AD). The goal of this experimental work was to investigate whether demodectic dogs could be previously exposed/sensitized to house dust mites’ antigens. First the prevalence of demodicosis in a southeastern region of Brazil was investigated by analyzing clinical files of dogs that were admitted to a Veterinary Hospital. Subsequently, the IgG responses to Dermatophagoides pteronyssinus (Dp) and Dermatophagoides farinae (Df) and IgE to D. pteronyssinus (Dp) were evaluated in two groups, AD or demodicosis dogs. Additionally, the major IgE-binding Dp proteins that are recognized by sera from dogs with demodicosis and AD were evaluated. A total of 2,599 clinical files were analyzed to identify the major parasitic skin diseases in dogs from this region, considering the age, sex and breed of the animals. The epidemiological study identified 111 animals with skin diseases; from these 20.7% presented demodicosis. Afterwards, serum samples were obtained from another groups of demodicosis, AD, and healthy dogs, and analyzed for Dp and Df-specific IgG, and IgE antibody levels, Dp IgG avidity by ELISA and IgE-binding Dp-specific proteins by immunoblot. IgG and IgE antibodies to Dp were detected in sera from additional groups of dogs with AD, demodicosis or healthy, with higher IgE levels to Dp in AD than demodectic or healthy dogs. IgG to Df was detected, despite with smaller levels compared to Dp in sera from demodectic dogs, and also in healthy dogs. Immunoblot showed IgE-binding to Dp proteins in sera of dogs with demodicosis and AD; with strong reactivity for the 72 and 116 kDa antigens detected by sera from demodicosis dogs. However, sera from healthy dogs >12 months old also presented reactivity to these bands. In conclusion, the detection of Dp-IgG and IgE antibodies in sera from demodectic dogs indicates previous exposure and sensitization to the house dust mite, respectively, more than cross-reactivity between demodex mites and Dp antigens detected by canine antibodies. Additionally, higher Dp-specific IgE levels were found in dogs with AD compared with those with demodicosis or healthy, suggesting that Dp-specific IgE could better discriminate dogs with AD from healthy ones or even those with demodicosis.

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Seasonal differences in airway hyperresponsiveness in asthmatic patients: relationship with allergen exposure and sensitization to house dust mites

Clinical & Experimental Allergy ◽

10.1046/j.1365-2222.1997.d01-554.x ◽

1997 ◽

Vol 27 (6) ◽

pp. 627-633 ◽

Cited By ~ 26

Author(s):

S. VAN DER HEIDE ◽

J. G. R. DE MONCHY ◽

K. DE VRIES ◽

A. E. J. DUBOIS ◽

H. F. KAUFFMAN

Keyword(s):

House Dust ◽

Airway Hyperresponsiveness ◽

Allergen Exposure ◽

House Dust Mites ◽

Dust Mites ◽

Seasonal Differences ◽

Asthmatic Patients

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Correlation Between Density of House Dust Mites and Recurrence of Urticaria

Jurnal Kesehatan Andalas ◽

10.25077/jka.v8.i3.p511-514.2019 ◽

2019 ◽

Vol 8 (3) ◽

pp. 511

Author(s):

Gardenia Akhyar ◽

Rahma Ledika Veroci

Keyword(s):

Skin Prick Test ◽

House Dust ◽

House Dust Mites ◽

Dust Mites ◽

Prick Test

Beberapa kasus urtikaria pada paparan debu dan diantaranya menghubungkan sensitivitas tungau debu rumah (TDR) dengan urtikaria berdasarkan riwayat pasien dan pemeriksaan uji intradermal. Tujuan penelitian ini adalah menentukan korelasi antara kepadatan TDR dan rekurensi urtikaria. Jumlah sampel pada penelitian ini 30 orang. Kepadatan TDR dari tiap kamar sampel dihitung dengan mengumpulkan debu, dan sampel dinilai sensitivitas terhadao TDR dengan pemeriksaan skin prick test (SPT). Diagnosis urtikaria berdasarkan anamnesis, kemudian ditanyaan rekurensinya dalam tiga bulan terakhir. Hasil: Dari 30 peserta, tujuh (23,3%) sampel memiliki kepadatan TDR sedang sementara 23 (76,7%) memiliki kepadatan rendah. Selain itu, 15 sampel positif SPT terhadap alergen TDR. Dari anamnesis, 12 (40%) sampel didiagnosis urtikaria dan diantaranya, 11 orang (91,67%) positif terhadap SPT. Tidak ada sampel yang memiliki rekurensi urtikaria dalam tiga bulan terakhir. Kesimpulan: Meskipun penelitian ini tidak menunjukkan korelasi yang signifikan antara kepadatan TDR dan tingkat kekambuhan urtikaria (p> 0,05), TDR memang bisa memicu eksaserbasi. Penelitian ini membutuhkan penyelidikan lebih lanjut dengan ukuran sampel yang lebih besar.

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