Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa

Deteksi dan Analisis Faktor Risiko Hipertensi pada Karyawan di Lingkungan Universitas Sriwijaya

Office workers are one group with a high proportion of hypertension compared to other types of work. Objectives: To determined the proportion of pre-hypertension, hypertension and risk factors for hypertension among employees in Sriwijaya University. Methods: This study used a cross-sectional design at Sriwijaya University, and samples were taken from 4 faculties totaling 152 respondents. Sampling using cluster random sampling technique. Data were collected from September to October 2020. The analysis was carried out in univariate, bivariate and multivariate analysis with logistic regression tests. Results: There were 13.2% of employees had hypertension, 40.8% had pre-hypertension, and the risk factor for hypertension among employees was age (p-value = 0.01; OR = 4.76; CI 1.50 <OR <15, 10) after controlling for the variables of the length of work, marriage status and cholesterol levels. Conclusion: There are more than 50% of employees are in a condition of pre-hypertension and hypertension, with age as the main risk; for this reason, employees who are 45 years old are expected to carry out routine health checks with the facilitation of the university and be able to implement the Dietary Approach to Stop hypertension in employees with pre-hypertension / hypertension.Keywords: DASH, hypertension, pre-hypertension, the risk factor

A Case of Tuberculous Spondylitis in Child with Undernourish

Tuberculous spondylitis accounts for around 2% of all cases of Tuberculosis (TB) and around 15% of extrapulmonary TB cases. It has been reported that a 17 years old boy with a complaint of a bump on the lumbar region and felt low back pain since two years before admission with a history of back trauma. There was a decrease of body weight. There was no paraesthesia nor paralysis. Defecation and micturition were normal. Basic immunization was incomplete. On physical examination found palpable lymph nodes 0,5x0,5x0,5 cm, multiple et regio colli. There was no BCG scar. Impression nutritional status was undernourished. There was fixed palpable mass at back size about 5x4x5 cm, hard, no fluctuations, no rebound tenderness. Lung examination was normal. Tuberculin test showed induration sized 20 mm. Gene Xpert result Micobacterium Tuberculosis (MTB) not detected. On chest X-ray examination found L1-2 corpus destruction. MRI Spine was suggestive of compressive fractures and suggestive of a bilateral psoas abscess. Decompression and lumbar stabilization surgery were performed. The histopathology examination results were consistent to spondylitis TB characteristics. The patient was discharged on 6th hospitalization and given anti-tuberculous drug.Keywords: bump, extrapulmonary, fracture, spondylitis, tuberculosis

Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on 15 drinking water at the Andalas University faculty canteens. Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number (MPN) tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

Perubahan Perilaku Hidup Bersih dan Sehat Sebelum dan Selama Pandemi COVID-19 pada Mahasiswa Kedokteran Universitas Muhammadiyah Jakarta

Novel Coronavirus (2019-nCoV) is a virus that causes respiratory disease COVID-19 (Coronavirus Disease-2019). COVID-19 can be transmitted through droplets when someone coughs or sneezes. Clean and healthy behavior is one way that can be done to prevent transmission of COVID-19. Objectives: To determined changes in clean and healthy behavior before and during the COVID-19 pandemic in 135 medical students of Muhammadiyah Jakarta University class of 2020. Methods: Data was collected by online questionnaire. The sampling technique was total sampling. Statistical tests were performed by using paired sample t-test. Results: There were changes in clean and healthy behavior before and during the COVID-19 pandemic. Conclusion: Clean and healthy behavior was better during the COVID-19 pandemic among respondents.Keywords: COVID-19, clean and healthy behavior, questionnaire

Perbandingan Tingkat Keparahan Infeksi Sekunder Virus Dengue pada Keempat Serotipe di Indonesia: Systematic Review

Secondary infection with the dengue virus causes mild to severe manifestations. The distribution of dengue virus serotypes varies in various areas and can change over time. There are four dengue serotypes, namely DENV-1, DENV-2, DENV-3 and DENV-4. Objectives: To knew the distribution of virus serotypes in an area and determined the pathogenesis of the disease, which can cause severe manifestations in patients with secondary infections. Methods: The data taken is the severity of secondary infections and dengue serotypes. The literature search was performed on PMC and Cochrane. Search criteria were performed using keywords (secondary infection * OR secondary dengue infection *) AND (Dengue Virus * OR Dengue Infection * OR Dengue * OR DENV) AND (Serotype * OR Serogroup) AND (severe dengue * OR severity * OR severity of illness indexs * OR dengue fever * OR dengue haemorrhage fever * OR dengue shock syndrome * OR DF * OR DHF * OR DSS *) AND (Indonesia *). Results: Literature study search found 387 literature with five studies conducted the analysis. From the results of the analysis, it was found that secondary infections were more common in patients with recurrent dengue infection with serotype 2 (DENV-2), serotype 3 (DENV-3) and serotype 4 (DENV-4). Conclusion: Secondary infection of dengue virus serotype 2 (DENV-2) and serotype 3 (DENV-3) can cause severe dengue infection.Keywords: Dengue Virus, Indonesia, Secondary Infection, Serotype, Severity

Perpaduan Isometric Exercise dan Latihan Pernafasan Yoga Terhadap Penurunan Tekanan Darah pada Pasien Hipertensi di Desa Mojojajar Wilayah Kerja Puskesmas Kedungsari Kabupaten Mojokerto

One of the non-pharmacological treatments that can be done to lower blood pressure is to increase the patient's physical activity, such as Isometric Exercise and Yoga breathing exercises. Objectives: To determined isometric exercise and yoga breathing on reducing blood pressure in hypertension. Methods: This study used a pre-experimental design with a one-group pretest-postest design. The sampling technique was purposive sampling. The number of samples in this study was 25 people. Results: The paired t-test statistical test results were a decrease in blood pressure after being given the intervention of isometric exercise and yoga breathing exercises with a p-value of 0.000 (p<0.05). Conclusion: Isometric exercise and yoga breathing exercises can be an alternative non-pharmacological therapy to reduce blood pressure in hypertension.Keywords: Isometric Exercise, Yoga Breathing Exercise, Hypertension

Perbandingan Perubahan Luas Luka dan Angiogenesis pada Jaringan Luka Bakar Derajat IIB Tikus Sprague Dawley yang Diberikan Platelet Rich Fibrin dan Advanced Platelet Rich Fibrin

Burns increase tissue damage by free radicals, thus hindering the healing process. Platelet Rich Fibrin (PRF) and Advanced Platelet Rich Fibrin (A-PRF) are autologous fibrin matrices that contain growth factors and have been proven to increase tissue formation and regeneration. Objectives: To determined the effect of applying PRF and A-PRF on deep second-degree burns. Methods: This study was experimental research on twenty male Sprague Dawley rats were randomly divided into four groups. Group A and B as control groups received NaCl 0,9% and silver sulfadiazine ointment, respectively. Group C was given PRF and group D was given A-PRF. Burns are photographed and the area was calculated using the ImageJ application. After 14 days of therapy, the burn areas were excised and made into histological slides. Microscopic study of angiogenesis was observed using a microscope at 400x magnification. Results: Changes in wound surface area in PRF and A-PRF groups were 3,106 cm and 2,333 cm. The number of angiogenesis in PRF and A-PRF groups were 3.88 and 5.88. The data was analyzed using One-Way Anova and the results showed that there were no significant differences between the changes in the wound area and angiogenesis of PRF and A-PRF groups. Conclusion: This study showed that there are no significant differences in changes in wound surface area and angiogenesis of burn injury after topical application of PRF and A-PRF.Keywords: angiogenesis, burn injury, changes in wound surface area, A-PRF, PRF

The Profile of COVID-19 Patients in Semen Padang Hospital Indonesia

The rapid increase in the number of Coronavirus Disease 2019 (COVID-19) cases, including in Padang, has led the government to designate several hospitals as referrals for COVID-19 patients. The profile of COVID-19 patients is needed to describe the pattern of this disease in Padang. Objectives: To revealed the profile of COVID-19 patients in SPH, Indonesia. Methods: This was a retrospective study. This study was conducted and collected data of COVID-19 patients from March to June 2020. Results: There are 54 patients with mean age of 50.93 (14.6) years old, 43% male and 57% female. The clinical manifestations are cough (74%), fever (37%), sore throat (22%), and fatigue (35%). There are 82% of patients admitted that they have contacted with other COVID-19 patients or have activities in COVID-19 cluster area, and only 7% have traveled to other cities. The mean length of stay is 31.91 (14.87) days. The mean laboratory data: Hemoglobin 13.7 (1.39) gr/dl, WBC 8,037.04 (2,846.27) /mm3, platelet 274,389 (75,053.80) /mm3, and NLR 3.16 (3.37). Chest radiograph results are bronchopneumonia (22.22%), cardiomegaly (7.41%), and normal (70.37%). Conclusion: The profile of COVID-19 patients in SPH is more in female patients, cough is the most clinical manifestation that appeared, the mean laboratory data (hemoglobin, WBC, and platelet) are normal, and normal chest radiography is the most chest radiograph results in these patients.Kata kunci: COVID-19, Padang, profile

Intoksikasi Alkohol Akibat Minuman Keras Oplosan

The incidence of alcoholic liquor poisoning (alcoholic liquor) in Indonesia shows a high number. One of the causes of alcohol poisoning is methanol. Methanol poisoning in Indonesia usually occurs as a result of drinking mixed alcohol liquor. It has been reported a male 23 years old with decreased consciousness after drinking oplosan alcohol suspected to contain methanol. The examination reveals that patients has metabolic acidosis, toxic optic neuropathy (TON) and erosive gastritis. Treatment was acidosis correction, prevent formation of metabolites, hemodialysis with supportive and symptomatic teraphy .Keywords: methanol, poisoning, alcohol mixed liquor