Modified Methylene Blue Screening Method for Significant Bacteriuria

The new adsorbents were prepared from Moroccan oil shale by chemical and physical process .In this study, experimental Plackett-Burman has been used as a screening method to study six factors for the development of materials to adsorbent basis of oil shale Moroccan. The factors have been identified by two levels, To Know temperature (°C), Processing time (min), mass ratio (m precursor/m acid), Pretreatment mixture the precursor with acid, origin of the raw material and type of the activating agent (H2SO4, H3PO4).And it was chosen as a response The maximum quantity of adsorption of the molecule of Methylene blue (Qads in mg/g) and the specific surface measure by the method bet (Sbet in m2/g), The predicted values were in agreement with the experimental values with a coefficient of determination (R2) of 0.98. The model has been validated by experiments subsequent to optimized conditions. The experimental data processing by software JMP 7 showed that the processing temperature The report of oil shale on the acid and activation time were the important effect on the maximal capacity of adsorption of methylene blue. The sample prepared at 237 °C during 215 min with pre-processing has a maximal capacity of adsorption equal to 54mg/g according to model of adsorption of Langmuir and SBET equal to 143 m2/g.

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The Methylene Blue Reduction Test: Evaluation of a Screening Method for Glucose-6-Phosphate Dehydrogenase Deficiency

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.1974.23.1197 ◽

1974 ◽

Vol 23 (6) ◽

pp. 1197-1202 ◽

Cited By ~ 3

Author(s):

W. N. Gibbs

Keyword(s):

Methylene Blue ◽

Dehydrogenase Deficiency ◽

Screening Method ◽

Test Evaluation ◽

Reduction Test ◽

Methylene Blue Reduction ◽

Glucose 6 Phosphate Dehydrogenase

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A New screening method of urine culture for assessing significant bacteriuria

Pathology ◽

10.1016/s0031-3025(16)39660-x ◽

1969 ◽

Vol 1 (2) ◽

pp. 164

Author(s):

E.M. Mackay-Scollay

Keyword(s):

Urine Culture ◽

Screening Method ◽

Significant Bacteriuria

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Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria

Journal of Clinical Microbiology ◽

10.1128/jcm.3.1.42-46.1976 ◽

1976 ◽

Vol 3 (1) ◽

pp. 42-46

Author(s):

D N Alexander ◽

G M Ederer ◽

J M Matsen

Keyword(s):

False Negative ◽

Screening Method ◽

Culture Method ◽

Clinical Microbiology Laboratory ◽

University Of Minnesota ◽

Atp Assay ◽

Negative Results ◽

False Negative Results ◽

Significant Bacteriuria ◽

Urine Specimens

The bioluminescent reaction of adenosine 5'-triphosphate (ATP) with luciferin and luciferase has been used in conjunction with a sensitive photometer (Lab-Line's ATP photometer) to detect significant bacteriuria in urine. This rapid method of screening urine specimens for bacteriuria was evaluated by using 348 urine specimens submitted to the clinical microbiology laboratory at the University of Minnesota Hospitals for routine culture using the calibrated loop-streak plate method. There was 89.4% agreement between the culture method and the ATP assay, with 7.0% false positive and 27.0% false negative results from the ATP assay using 10(5) organisms/ml of urine or greater as positive for significant bacteriuria and less than 10(5) organisms/ml as negative for significant bacteriuria.

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A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065833 ◽

1971 ◽

Vol 29 ◽

pp. 358-359

Author(s):

B. J. Panessa ◽

J. F. Gennaro

Keyword(s):

Methylene Blue ◽

Toluidine Blue ◽

Outer Surface ◽

Microscope Study ◽

Light Microscope ◽

Carnivorous Plant ◽

Sarracenia Purpurea ◽

Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

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An integrated screening method for evaluating the pulmonary toxicity of inhaled particulates: Role of microscopic techniques in assessing pulmonary macrophage clearance functions

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161692 ◽

1990 ◽

Vol 48 (3) ◽

pp. 826-827

Author(s):

David B. Warheit ◽

Lena Achinko ◽

Mark A. Hartsky

Keyword(s):

Bronchoalveolar Lavage ◽

Pulmonary Toxicity ◽

Screening Method ◽

Pulmonary Response ◽

Inhaled Particles ◽

Cytochemical Analysis ◽

Pulmonary Macrophages ◽

Integrated Screening

There is a great need for the development of a rapid and reliable bioassay to evaluate the pulmonary toxicity of inhaled particles. A number of methods have been proposed, including lung clearance studies, bronchoalveolar lavage analysis, and in vitro cytotoxicity tests. These methods are often limited in scope inasmuch as they measure only one dimension of the pulmonary response to inhaled, instilled or incubated dusts. Accordingly, a comprehensive approach to lung toxicity studies has been developed.To validate the method, rats were exposed for 6 hours or 3 days to various concentrations of either aerosolized alpha quartz silica (Si) or carbonyl iron (CI) particles. Cells and fluids from groups of sham and dust-exposed animals were recovered by bronchoalveolar lavage (BAL). Alkaline phosphatase, LDH and protein values were measured in BAL fluids at several time points postexposure. Cells were counted and evaluated for viability, as well as differential and cytochemical analysis. In addition, pulmonary macrophages (PM) were cultured and studied for morphology, chemotaxis, and phagocytosis by scanning electron microscopy.

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