Mass detection of significant bacteriuria. An improved triphenyltetrazolium chloride (TTC) technique

1969 ◽  
Vol 124 (2) ◽  
pp. 165-169 ◽  
Author(s):  
B. Resnick
1970 ◽  
Vol 25 (11) ◽  
pp. 1047-1049
Author(s):  
Bernard Resnick ◽  
Robert L. Cella ◽  
Krikor Soghikian ◽  
Amos H. Lieberman ◽  
Eli Weil

2020 ◽  
Vol 500 (3) ◽  
pp. 3920-3925
Author(s):  
Wolfgang Brandner ◽  
Hans Zinnecker ◽  
Taisiya Kopytova

ABSTRACT Only a small number of exoplanets have been identified in stellar cluster environments. We initiated a high angular resolution direct imaging search using the Hubble Space Telescope (HST) and its Near-Infrared Camera and Multi-Object Spectrometer (NICMOS) instrument for self-luminous giant planets in orbit around seven white dwarfs in the 625 Myr old nearby (≈45 pc) Hyades cluster. The observations were obtained with Near-Infrared Camera 1 (NIC1) in the F110W and F160W filters, and encompass two HST roll angles to facilitate angular differential imaging. The difference images were searched for companion candidates, and radially averaged contrast curves were computed. Though we achieve the lowest mass detection limits yet for angular separations ≥0.5 arcsec, no planetary mass companion to any of the seven white dwarfs, whose initial main-sequence masses were >2.8 M⊙, was found. Comparison with evolutionary models yields detection limits of ≈5–7 Jupiter masses (MJup) according to one model, and between 9 and ≈12 MJup according to another model, at physical separations corresponding to initial semimajor axis of ≥5–8 au (i.e. before the mass-loss events associated with the red and asymptotic giant branch phase of the host star). The study provides further evidence that initially dense cluster environments, which included O- and B-type stars, might not be highly conducive to the formation of massive circumstellar discs, and their transformation into giant planets (with m ≥ 6 MJup and a ≥6 au). This is in agreement with radial velocity surveys for exoplanets around G- and K-type giants, which did not find any planets around stars more massive than ≈3 M⊙.


Author(s):  
He Xiao ◽  
Qingfeng Wang ◽  
Zhiqin Liu ◽  
Jun Huang ◽  
Yuwei Zhou ◽  
...  
Keyword(s):  

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
R. Gopinath ◽  
S. T. Narenderan ◽  
M. Kumar ◽  
B. Babu

AbstractA simple, sensitive, and specific liquid chromatography-tandem mass spectrophotometry (LC-MS/MS) method was developed and validated for the quantification of lenalidomide in human plasma. The separation was carried out on a symmetry, C18, 5-μm (50 × 4.6 mm) column as stationary phase and with an isocratic mobile phase of 0.1% formic acid in water-methanol in the ratio of (15:85, v/v) at a flow rate of 0.5 mL/min. Protonated ions formed by electrospray ionization in the positive mode were used to detect analyte and fluconazole (internal standard). The mass detection was made by monitoring the fragmentation of m/z 260.1/148.8 for lenalidomide and m/z 307.1/238.0 for internal standard on a triple quadrupole mass spectrometer. The developed method was validated over the concentration range of 10–1000 ng/mL for lenalidomide in human plasma with a correlation coefficient (r2) was 0.9930. The accuracy and precision values obtained from six different sets of quality control samples analyzed on separate occasions ranged from 99.41 to 106.97% and 2.88 to 4.22%, respectively. Mean extraction recoveries were 98.06% and 88.78% for the analyte and IS, respectively. The developed method was successfully applied for analyzing lenalidomide in human plasma samples.


Open Physics ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 927-934
Author(s):  
Tao Song ◽  
Chao Liu ◽  
Hengxuan Zhu ◽  
Min Zeng ◽  
Jin Wang

Abstract Normal operation of gas turbines will be affected by deposition on turbine blades from particles mixed in fuels. This research shows that it is difficult to monitor the mass of the particles deposition on the wall surface in real time. With development of electronic technology, the antenna made of printed circuit board (PCB) has been widely used in many industrial fields. Microstrip antenna is first proposed for monitoring particles deposition to analyse the deposition law of the particles accumulated on the wall. The simulation software Computer Simulation Technology Microwave Studio (CST MWS) 2015 is used to conduct the optimization design of the PCB substrate antenna. It is found that the S11 of vivaldi antenna with arc gradient groove exhibits a monotonous increase with the increase of dielectric layer thickness, and this antenna is highly sensitive to the dielectric layer thickness. Moreover, a cold-state test is carried out by using atomized wax to simulate the deposition of pollutants. A relationship as a four number of times function is found between the capacitance and the deposited mass. These results provide an important reference for the mass detection of the particle deposition on the wall, and this method is suitable for other related engineering fields.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoonhee Kim ◽  
Yoon Bum Lee ◽  
Seung Kuk Bae ◽  
Sung Suk Oh ◽  
Jong-ryul Choi

AbstractPhotochemical thrombosis is a method for the induction of ischemic stroke in the cerebral cortex. It can generate localized ischemic infarcts in the desired region; therefore, it has been actively employed in establishing an ischemic stroke animal model and in vivo assays of diagnostic and therapeutic techniques for stroke. To establish a rabbit ischemic stroke model and overcome the shortcoming of previous studies that were difficult to build a standardized photothrombotic rabbit model, we developed a photochemical thrombosis induction system that can produce consistent brain damage on a specific area. To verify the generation of photothrombotic brain damage using the system, longitudinal magnetic resonance imaging, 2,3,5-triphenyltetrazolium chloride staining, and histological staining were applied. These analytical methods have a high correlation for ischemic infarction and are appropriate for analyzing photothrombotic brain damage in the rabbit brain. The results indicated that the photothrombosis induction system has a main advantage of being accurately controlled a targeted region of photothrombosis and can produce cerebral hemisphere lesions on the target region of the rabbit brain. In conjugation with brain atlas, it can induce photochemical ischemic stroke locally in the part of the brain that is responsible for a particular brain function and the system can be used to develop animal models with degraded specific functions. Also, the photochemical thrombosis induction system and a standardized rabbit ischemic stroke model that uses this system have the potential to be used for verifications of biomedical techniques for ischemic stroke at a preclinical stage in parallel with further performance improvements.


ChemPlusChem ◽  
2015 ◽  
Vol 80 (5) ◽  
pp. 839-850 ◽  
Author(s):  
Petr Žáček ◽  
Jiří Kindl ◽  
Kateřina Frišonsová ◽  
Markéta Průchová ◽  
Alena Votavová ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
pp. 76
Author(s):  
Flavia A. Tejeda-Bayron ◽  
David E. Rivera-Aponte ◽  
Christian J. Malpica-Nieves ◽  
Gerónimo Maldonado-Martínez ◽  
Héctor M. Maldonado ◽  
...  

Stroke is one of the leading causes of long-term disability. During ischemic stroke, glutamate is released, reuptake processes are impaired, and glutamate promotes excitotoxic neuronal death. Astrocytic glutamate transporter 1 (GLT-1) is the major transporter responsible for removing excess glutamate from the extracellular space. A translational activator of GLT-1, LDN/OSU 0212320 (LDN) has been previously developed with beneficial outcomes in epileptic animal models but has never been tested as a potential therapeutic for ischemic strokes. The present study evaluated the effects of LDN on stroke-associated brain injury. Male and female mice received LDN or vehicle 24 h before or 2 h after focal ischemia was induced in the sensorimotor cortex. Sensorimotor performance was determined using the Rung Ladder Walk and infarct area was assessed using triphenyltetrazolium chloride staining. Males treated with LDN exhibited upregulated GLT-1 protein levels, significantly smaller infarct size, and displayed better sensorimotor performance in comparison to those treated with vehicle only. In contrast, there was no upregulation of GLT-1 protein levels and no difference in infarct size or sensorimotor performance between vehicle- and LDN-treated females. Taken together, our results indicate that the GLT-1 translational activator LDN improved stroke outcomes in young adult male, but not female mice.


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