Prevalence and Multiplex PCR for Enterotoxin Genes of Staphylococcus aureus Isolates from Subclinical Mastitis and Kareish Cheese

This study is aimed to determine the prevalence of staphylococcus aureus (S.aureus) by biochemical tests in poultry carcasses. It is also intend to detect the distribution of genes for classical staphylococcal enterotoxins A, B, C, D and E (sea, seb, sec, sed and see) and for gene femA, specific for S.aureus species, using multiplex PCR. A total of 385 samples of neck skins from fresh poultry carcasses were collected during the period 2012-2013 from 16 different slaughterhouses located in the region of Algiers, Algeria. The overall prevalence of S.aureus in freshly slaughtered poultry carcasses was 41.56%, with an individual prevalence of 40.63% and 45.71% for chicken and turkey respectively. From the 95 strains of S.aureus identified by biochemical tests, 82 (86.32%) isolates were femA positive using multiplex PCR. The investigation has also revealed the presence of both enterotoxins B and D, with a predominance of seb (13.33%) followed by sed (1.67%), in the chicken carcasses while in turkey only sed was detected (4.55%) It has been found that strains of S.aureus of poultry origin can be enterotoxigenic with the predominance of genes encoding for enterotoxins seb in chicken and sed in turkey. As enterotoxins can be produced in adequate amounts to induce foodborne illnesses, these potential dangers must be considered in terms of a real risk to public health.

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Detection of biofilm related genes, classical enterotoxin genes and agr typing among Staphylococcus aureus isolated from bovine with subclinical mastitis in southwest of Iran

Microbial Pathogenesis ◽

10.1016/j.micpath.2016.05.022 ◽

2016 ◽

Vol 97 ◽

pp. 45-51 ◽

Cited By ~ 17

Author(s):

Seyed Sajjad Khoramrooz ◽

Fariba Mansouri ◽

Masoud Marashifard ◽

Seyed Ali Asghar Malek Hosseini ◽

Fereshteh Akbarian Chenarestane-Olia ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Subclinical Mastitis ◽

Enterotoxin Genes ◽

Southwest Of Iran

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Virulence gene profiles inStaphylococcus aureusisolated from cows with subclinical mastitis in eastern Poland

Journal of Dairy Research ◽

10.1017/s002202991600008x ◽

2016 ◽

Vol 83 (2) ◽

pp. 228-235 ◽

Cited By ~ 13

Author(s):

Barbara Kot ◽

Piotr Szweda ◽

Aneta Frankowska-Maciejewska ◽

Małgorzata Piechota ◽

Katarzyna Wolska

Keyword(s):

Staphylococcus Aureus ◽

Serine Protease ◽

Virulence Genes ◽

Bovine Mastitis ◽

Virulence Gene ◽

Subclinical Mastitis ◽

Staph Aureus ◽

Gene Profiles ◽

Enterotoxin Genes ◽

Genes Encoding

Staphylococcus aureusis arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124Staph. aureusisolates from subclinical mastitis in cows in eastern Poland. The presence of 30 virulence genes encoding adhesins, proteases and superantigenic toxins was investigated by PCR. The 17 different combinations of adhesin genes were identified. Occurrence ofeno(91·1%) andfib(82·3%) genes was found to be common. The frequency of other adhesion genesfnbA, fnbB, ebpswere 14·5, 50, 25%, respectively, and forcnaandbbpwere 1·6%. TheetAandetDgenes, encoding exfoliative toxins, were present in genomes of 5·6 and 8·9% isolates, respectively. ThesplAandsspA, encoding serine protease, were detected in above 90% isolates. The most frequent enterotoxin genes weresei(21%),sem(19·4%),sen(19·4%),seg(18·5%) andseo(13·7%). Thetstgene was harboured by 2·4% isolates. The 19 combinations of the superantigenic toxin genes were obtained and found in 35·5% of isolates. Three of them (seg, sei, sem, sen, seo; sec, seg, sei, sem, sen, seoandseg, sei, sem, sen) were the most frequent and found in 16·1% of the isolates. The most common virulotype, present in 17·7% of the isolates, wasfib, eno, fnbB, splA, splE, sspA. The results indicate the variation in the presence of virulence genes inStaph. aureusisolates and considerable diversity of isolates that are able to cause mastitis in cows.

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A strategy based on 5′ nuclease multiplex PCR to detect enterotoxin genes sea to sej of Staphylococcus aureus

Molecular and Cellular Probes ◽

10.1016/s0890-8508(03)00058-6 ◽

2003 ◽

Vol 17 (5) ◽

pp. 227-235 ◽

Cited By ~ 25

Author(s):

Capucine Letertre ◽

Sylvie Perelle ◽

Françoise Dilasser ◽

Patrick Fach

Keyword(s):

Staphylococcus Aureus ◽

Multiplex Pcr ◽

Enterotoxin Genes

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DETECTION OF STAPHYLOCOCCUS AUREUS CLASSIC ENTEROTOXIN GENES IN SOME MEAT PRODUCTS USING MULTIPLEX PCR

Kafrelsheikh Veterinary Medical Journal ◽

10.21608/kvmj.2016.108554 ◽

2016 ◽

Vol 14 (1) ◽

pp. 47-58

Author(s):

N. Y. Moustafa ◽

Azza, M.M Deeb ◽

Seham, N. Homouda ◽

Shimaa, M. Abd El-Bar

Keyword(s):

Staphylococcus Aureus ◽

Multiplex Pcr ◽

Meat Products ◽

Enterotoxin Genes

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Enterotoxicity and genetic variation among clinical Staphylococcus aureus isolates in Jordan

Journal of Medical Microbiology ◽

10.1099/jmm.0.46183-0 ◽

2006 ◽

Vol 55 (2) ◽

pp. 183-187 ◽

Cited By ~ 29

Author(s):

Randa G. Naffa ◽

Salwa M. Bdour ◽

Hussein M. Migdadi ◽

Asem A. Shehabi

Keyword(s):

Genetic Diversity ◽

Staphylococcus Aureus ◽

Genetic Variation ◽

Multiplex Pcr ◽

Dna Fingerprinting ◽

Rapd Analysis ◽

Clinical Isolates ◽

Randomly Amplified Polymorphic Dna ◽

Toxin Genes ◽

Enterotoxin Genes

A total of 100 Jordanian clinical Staphylococcus aureus isolates was analysed for the presence of the enterotoxin genes sea, seb, sec, sed and see using multiplex PCR. Twenty-three isolates (23 %) were potentially enterotoxigenic. The prevalence of sea, sec and sea plus sec among the total clinical isolates was 15, 4 and 4 %, respectively. None of the isolates harboured sed, seb or see genes. S. aureus isolates were subjected to DNA fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis to test whether isolates harbouring the toxin genes were genetically clustered. A total of 13 genotypes was identified at a 47 % similarity level. Genotypes I and V accounted for the largest number of enterotoxigenic isolates (19 %). This study has demonstrated the genetic diversity of Jordanian clinical S. aureus isolates and shown that the presence of the toxin genes is not genotype specific.

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Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/bvip-2014-0086 ◽

2014 ◽

Vol 58 (4) ◽

pp. 559-561 ◽

Cited By ~ 2

Author(s):

Weronika Korpysa-Dzirba ◽

Jacek Osek

Keyword(s):

Staphylococcus Aureus ◽

Multiplex Pcr ◽

Raw Milk ◽

Detectable Level ◽

The South ◽

Staphylococcal Enterotoxins ◽

East Region ◽

Enterotoxin Genes ◽

Toxin Protein

Abstract The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins (SEs) A - E. A total of 168 of S. aureus isolates from raw milk collected in the south - east region of Poland (Lubelskie Province) were tested for SE production by the ELFA, while multiplex PCR was applied for detection of enterotoxin genes (sea, seb, sec, sed, see). It was found that 20 (11.9%) out of 168 strains were positive for one or more classical SE markers and 19 of them produced a detectable level of enterotoxins. The results obtained by mPCR and ELFA were in agreement, when the presence of A, B, and D toxin types was tested; whereas SEC was not found by the ELFA although the S. aureus was positive for the respective gene. The results of the two methods showed that mPCR identified one more strain potentially producing enterotoxin than the ELFA, which may suggest that the enterotoxigenic S. aureus are not always able to express the toxin protein.

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