scholarly journals Prevalence and distribuion of staphylococcal enterotoxin genes among Staphylococcus aureus isolates from chicken and turkey carcasses in Algeria

2019 ◽  
Vol 69 (4) ◽  
pp. 1297 ◽  
Author(s):  
F. Mebkhout ◽  
L. Mezali ◽  
T. M. Hamdi ◽  
Z. Cantekin ◽  
Y. Ergun ◽  
...  
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Staphylococcal Enterotoxin ◽  
Foodborne Illnesses ◽  
Biochemical Tests ◽  
Staphylococcal Enterotoxins ◽  
Enterotoxin Genes ◽  
Real Risk ◽  
Genes Encoding

This study is aimed to determine the prevalence of staphylococcus aureus (S.aureus) by biochemical tests in poultry carcasses. It is also intend to detect the distribution of genes for classical staphylococcal enterotoxins A, B, C, D and E (sea, seb, sec, sed and see) and for gene femA, specific for S.aureus species, using multiplex PCR. A total of 385 samples of neck skins from fresh poultry carcasses were collected during the period 2012-2013 from 16 different slaughterhouses located in the region of Algiers, Algeria. The overall prevalence of S.aureus in freshly slaughtered poultry carcasses was 41.56%, with an individual prevalence of 40.63% and 45.71% for chicken and turkey respectively. From the 95 strains of S.aureus identified by biochemical tests, 82 (86.32%) isolates were femA positive using multiplex PCR. The investigation has also revealed the presence of both enterotoxins B and D, with a predominance of seb (13.33%) followed by sed (1.67%), in the chicken carcasses while in turkey only sed was detected (4.55%) It has been found that strains of S.aureus of poultry origin can be enterotoxigenic with the predominance of genes encoding for enterotoxins seb in chicken and sed in turkey. As enterotoxins can be produced in adequate amounts to induce foodborne illnesses, these potential dangers must be considered in terms of a real risk to public health.

scholarly journals Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

2014 ◽  
Vol 17 (3) ◽  
pp. 407-411 ◽  
Author(s):  
M. Piechota ◽  
B. Kot ◽  
E. Zdunek ◽  
J. Mitrus ◽  
J. Wicha ◽  
...  
Keyword(s):  
Public Health ◽  
Bovine Milk ◽  
Coagulase Negative Staphylococci ◽  
Staphylococcal Enterotoxins ◽  
Enterotoxin Genes ◽  
Genes Encoding ◽  
Enterotoxin B ◽  
Staphylococcus Spp

AbstractThe aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes.The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes.The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.

scholarly journals Prevalence and expression of staphylococcal enterotoxin genes in Staphylococcus aureus isolated from food poisoning outbreaks

2017 ◽  
Vol 63 (10) ◽  
pp. 834-840 ◽  
Author(s):  
Caroline Peixoto Bastos ◽  
Milena Tomasi Bassani ◽  
Marcia Magalhães Mata ◽  
Graciela Volz Lopes ◽  
Wladimir Padilha da Silva
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Rio Grande ◽  
Staphylococcal Enterotoxin ◽  
The State ◽  
Transcriptional Expression ◽  
Rio Grande Do Sul ◽  
Staphylococcal Enterotoxins ◽  
Enterotoxin Genes ◽  
Reference Strains

Staphylococcus aureus is an important pathogen of foodborne origin. The pathogen produces a variety of toxins that include the staphylococcal enterotoxins (SE). The present study aimed to evaluate the prevalence and expression of 5 SE genes (sea, seb, sec, sed, and see) in S. aureus isolated from outbreaks occurred in the state of Rio Grande do Sul, Brazil. All isolates, with the exception of 2, presented the same or higher transcriptional expression than the reference strains for at least 1 of these genes. The presence of SE genes combined with high levels of transcriptional expression suggests that 1 or more SEs were involved with the staphylococcal food poisoning outbreak analyzed in the present study.

scholarly journals Detection of classical genes and enterotoxins of Staphylococcus aureus isolated from raw milk in the south-east region of Poland

2014 ◽  
Vol 58 (4) ◽  
pp. 559-561 ◽  
Author(s):  
Weronika Korpysa-Dzirba ◽  
Jacek Osek
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Raw Milk ◽  
Detectable Level ◽  
The South ◽  
Staphylococcal Enterotoxins ◽  
East Region ◽  
Enterotoxin Genes ◽  
Toxin Protein

Abstract The aim of the study was to investigate if the enterotoxigenic strains of S. aureus isolated from raw milk are able to produce staphylococcal enterotoxins (SEs) A - E. A total of 168 of S. aureus isolates from raw milk collected in the south - east region of Poland (Lubelskie Province) were tested for SE production by the ELFA, while multiplex PCR was applied for detection of enterotoxin genes (sea, seb, sec, sed, see). It was found that 20 (11.9%) out of 168 strains were positive for one or more classical SE markers and 19 of them produced a detectable level of enterotoxins. The results obtained by mPCR and ELFA were in agreement, when the presence of A, B, and D toxin types was tested; whereas SEC was not found by the ELFA although the S. aureus was positive for the respective gene. The results of the two methods showed that mPCR identified one more strain potentially producing enterotoxin than the ELFA, which may suggest that the enterotoxigenic S. aureus are not always able to express the toxin protein.

scholarly journals Prevalence of methicillin-resistant Staphylococcus aureus and pattern of antimicrobial resistance in mastitis milk of cattle in Chitwan, Nepal

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Asmita Shrestha ◽  
Rebanta Kumar Bhattarai ◽  
Himal Luitel ◽  
Surendra Karki ◽  
Hom Bahadur Basnet
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Subclinical Mastitis ◽  
World Health ◽  
Dairy Herds ◽  
Microbiological Analysis ◽  
Biochemical Tests ◽  
Methicillin Resistant ◽  
Milk Samples

Abstract Background The threat of methicillin-resistant Staphylococcus aureus (MRSA) exists globally and has been listed as a priority pathogen by the World Health Organization. One of the sources of MRSA emergence is livestock and its products, often raised in poor husbandry conditions. There are limited studies in Nepal to understand the prevalence of MRSA in dairy animals and its antimicrobial resistance (AMR) profile. A cross-sectional study was conducted in Chitwan, one of the major milk-producing districts of Nepal, from February 2018 to September 2019 to estimate the prevalence of MRSA in milk samples and its AMR profile. The collected milk samples (n = 460) were screened using the California Mastitis Test (CMT) and positive samples were subjected to microbiological analysis to isolate and identify S. aureus. Polymerase Chain Reaction (PCR) was used to identify the presence of the mecA gene and screen for MRSA. Results In total, 41.5% (191/460) of milk samples were positive in the CMT test. Out of 191 CMT positive milk samples, the biochemical tests showed that the prevalence of S. aureus was 15.2% (29/191). Among the 29 S. aureus isolates, 6.9% (2/29) were identified as MRSA based on the detection of a mecA gene. This indicates that that 1.05% (2/191) of mastitis milk samples had MRSA. The antibiotic sensitivity test showed that 75.9% (22/29) and 48.3% (14/29) S. aureus isolates were found to be sensitive to Cefazolin and Tetracycline respectively (48.3%), whereas 100% of the isolates were resistant to Ampicillin. In total 96.6% (28/29) of S. aureus isolates were multidrug-resistant (MDR). Conclusions This study revealed a high prevalence of S. aureus-mediated subclinical mastitis in dairy herds in Chitwan, Nepal, with a small proportion of it being MRSA carrying a mecA gene. This S. aureus, CoNS, and MRSA contaminated milk poses a public health risk due to the presence of a phenotype that is resistant to very commonly used antibiotics. It is suggested that dairy herds be screened for subclinical mastitis and treatments for the animals be based on antibiotic susceptibility tests to reduce the prevalence of AMR. Furthermore, future studies should focus on the Staphylococcus spp. to explore the antibiotic resistance genes in addition to the mecA gene to ensure public health.

scholarly journals Ocurrence of Staphylococcus aureus and multiplex pcr detection of classic enterotoxin genes in cheese and meat products

2009 ◽  
Vol 40 (1) ◽  
pp. 145-148 ◽  
Author(s):  
Marcia Regina Pelisser ◽  
Cátia Silene Klein ◽  
Kelen Regina Ascoli ◽  
Thaís Regina Zotti ◽  
Ana Carolina Maisonnave Arisi
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Meat Products ◽  
Pcr Detection ◽  
Enterotoxin Genes

scholarly journals Analysis of home dust for Staphylococcus aureus and staphylococcal enterotoxin genes using quantitative PCR

2017 ◽  
Vol 581-582 ◽  
pp. 750-755 ◽  
Author(s):  
Shanna Ludwig ◽  
Isabel Jimenez-Bush ◽  
Emily Brigham ◽  
Sonali Bose ◽  
Gregory Diette ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Quantitative Pcr ◽  
Staphylococcal Enterotoxin ◽  
Enterotoxin Genes

scholarly journals Prevalence of classical Staphylococcal enterotoxin genes of Staphylococcus aureus isolated from ready-to-eat food in Ho Chi Minh City, Vietnam

2020 ◽  
Vol 3 (4) ◽  
pp. 9-15
Author(s):  
Phuc Nguyen Do ◽  
Linh Dang Thuy ◽  
My Huong Le Thi ◽  
Thuy Tran Thi ◽  
Ngoc Bong Nguyen Thi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Fast Food ◽  
Staphylococcal Enterotoxin ◽  
Ho Chi Minh City ◽  
Food Services ◽  
Hygiene Practices ◽  
Enterotoxin Genes ◽  
Three Samples ◽  
Random Samples ◽  
Good Hygiene

Contamination of Staphylococcus aureus in ready-to-eat (RTE) food is a leading cause of foodborne illness in Vietnam. The aim of this study was to analyze the frequency of classical Staphylococcal enterotoxin genes (sea, seb, sec, sed, and see genes) in S. aureus strains isolated from ready-to-eat food in Ho Chi Minh, Vietnam (76 strains of  S. aureus were isolated from 200 random samples of ready - to - eat (RTE) food, which include pate, pork roll, barbecue pork, roasted pork and roasted duck, 40 samples of each), all the samples were collected from different fast food services on street in district 5, 6, 7 and 8 in Ho Chi Minh City, Vietnam. All the trains of S. aureus were stored at -70oC for analyzing Staphylococcal enterotoxin genes). The obtained results in this study indicated that out of 76 S. aureus strains, there are 6 strains (7.9%) carried SE genes:  sea gene (1.32%) was detected in one pate sample (Dist. 8), sec gene was detected (3.95%) in three samples, which are two pork roll samples (Dist. 7 and 8) and one roasted duck sample (Dist. 8), and finally see gene was detected in one pate sample (Dist. 8)and in one pork roll sample (Dist.  8). This could be a serious public health risk and highlight the need to implement good hygiene practices.

Enzyme Immunoassay for Staphylococcal Enterotoxin A

1980 ◽  
Vol 63 (5) ◽  
pp. 1138-1143
Author(s):  
Peter E Kauffman
Keyword(s):  
Staphylococcus Aureus ◽  
Alkaline Phosphatase ◽  
Enzyme Immunoassay ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Cross Reactions ◽  
Staphylococcal Enterotoxins ◽  
Staphylococcus Aureus Cowan

Abstract An enzyme immunoassay procedure specifying alkaline phosphatase-labeled enterotoxin A was used to determine enterotoxin in standardized solutions and food extracts. Staphylococcus aureus Cowan 1 cells were used to separate unbound from antibody-bound enterotoxin. The method is sensitive to 2 ng enterotoxin A/mL; cross reactions with other staphylococcal enterotoxins did not interfere with the specificity. The method is sensitive and precise enough to serve as a reliable alternative to radioimmunoassay for enterotoxin A.

scholarly journals Prevalence of Staphylococcus aureus and staphylococcal enterotoxins at different stages of production of raw milk cheeses - preliminary results

2013 ◽  
Vol 57 (3) ◽  
pp. 341-345 ◽  
Author(s):  
Jolanta G. Rola ◽  
Weronika Korpysa-Dzirba ◽  
Jacek Osek
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Farms ◽  
Raw Milk ◽  
Preliminary Results ◽  
Staphylococcal Enterotoxins ◽  
Potential Sources ◽  
Genes Encoding ◽  
Coagulase Positive Staphylococci ◽  
Sources Of Contamination

Abstract The aim of the study was to identify the potential sources of contamination of traditionally made cheeses during their production with Staphylococcus aureus . The samples were collected at nine dairy farms at different points of manufacturing the cheeses. Isolation and enumeration of coagulase positive staphylococci (CPS) on Baird- Parker RPF agar was conducted, and detection of staphylococcal enterotoxins (SEs) was performed using ELISA and ELFA. The genes encoding SEs were identified by PCR. CPS were isolated from 51 samples with the highest level of contamination in mature cheese up to 107 CFU g-1. No SEs were detected in tested samples; however, enterotoxic CPS strains were found.

Sign in / Sign up

Export Citation Format

Share Document