Effect of an egg yolk immunoglobulin（IgY）product on oocyst shedding and blood and fecal IgY concentrations in <i>cryptosporidium</i>-infected calves

AbstractIn the present study, a sandwich ELISA based on IgY (egg yolk immunoglobulin) was developed for detection of circulating antigens (CAg) in sere of mice experimentally infected with Trichinella spiralis. The IgY-sandwich ELISA assay involved the use of chicken antibody IgY against excretory-secretory (ES) antigens of Trichinella spiralis muscle larvae as a capture antibody and mouse polyclonal antibody IgG to ES antigens as a detecting antibody. This method was able to detect as little as 3 ng/ml of ES antigens added to normal mouse serum. A group of sixteen mice was orally inoculated with 500 T. spiralis muscle larvae per animal. The serum samples from the infected mice were taken during 1–35 days post-infection (dpi). The CAg was detectable as early as 8 dpi in the sera of infected mice. The level of CAg increased dramatically during 13–15 dpi and reached a peak at 22 dpi and remained a plateau for 3 days, then declined gradually. Another peak of CAg occurred at 31 dpi. The anti-Trichinella antibodies was first detected in 14.3 % of the infected mice at 2 weeks post-infection (wpi), and reached a peak positive rate of 100 % at 5 wpi. Moreover, the infected mice were treated with abendazole at 5 wpi and the serum CAg levels increased significantly during 2–6 days posttreatment (dpt) and then declined rapidly during 8–14 dpt. By 42 dpt, the CAg levels decreased to the undetected level, but the detection rate of antibodies was still 100 %. The IgY-sandwich ELISA appears to be a sensitive for detection of antigenemia of T. spiralis and valuable to judge the efficacy of chemotherapy in trichinellosis.

Download Full-text

Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis

Microbial Pathogenesis ◽

10.1016/j.micpath.2019.103741 ◽

2019 ◽

Vol 137 ◽

pp. 103741 ◽

Cited By ~ 1

Author(s):

Yire Xiao ◽

Qingqing Hu ◽

Luoying Jiao ◽

Xiping Cui ◽

Panpan Wu ◽

...

Keyword(s):

Trichophyton Rubrum ◽

Therapeutic Potential ◽

Egg Yolk ◽

Egg Yolk Immunoglobulin

Download Full-text

Enterovirus type 71‑immunized chicken egg yolk immunoglobulin has cross antiviral activity against coxsackievirus A16 in�vitro

Experimental and Therapeutic Medicine ◽

10.3892/etm.2019.7529 ◽

2019 ◽

Cited By ~ 1

Author(s):

Enyi Gao ◽

Shuwen Wu ◽

Qing Xu ◽

Yonglian Zeng ◽

Ning Tan ◽

...

Keyword(s):

Antiviral Activity ◽

Egg Yolk ◽

Coxsackievirus A16 ◽

Chicken Egg ◽

Egg Yolk Immunoglobulin ◽

Enterovirus Type ◽

Chicken Egg Yolk

Download Full-text

Egg yolk immunoglobulin Y as a promising tool to detect immune complexes in neurocysticercosis serum samples

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1093/trstmh/traa028 ◽

2020 ◽

Vol 114 (8) ◽

pp. 585-592

Author(s):

Gabriela B da Silva ◽

Lucas S da Faria ◽

Camila A Lopes ◽

Daniela S Nunes ◽

Vanessa S Ribeiro ◽

...

Keyword(s):

Area Under The Curve ◽

Egg Yolk ◽

Enzyme Linked Immunosorbent Assay ◽

Immunoglobulin Y ◽

Likelihood Ratios ◽

Serum Samples ◽

Promising Tool ◽

Egg Yolk Immunoglobulin ◽

Potential Applicability ◽

Sensitivity Specificity

Abstract Background Neurocysticercosis (NCC) is a neglected tropical disease and its diagnosis is still a challenge due to non-specific manifestations. Neuroimaging techniques are used in the diagnosis of NCC, however, due to the high cost of these methods and the advantages presented in the use of immunological tests, such as ease of performance and satisfactory results, immunoassays are commonly used to detect antibodies against Taenia sp. antigens. The aim of the present study was to produce, characterize and apply specific polyclonal immunoglobulin Y (IgY) anti-Taenia crassiceps extracted from egg yolk of hens immunized with T. crassiceps metacestodes. Methods Indirect enzyme-linked immunosorbent assay (ELISA), avidity ELISA, immunoblotting and indirect immunofluorescence tests were performed for characterization of IgY antibodies. Diagnostic performance was verified by ELISA for immune complex detection testing 90 serum samples. Results Values of sensitivity, specificity, positive and negative likelihood ratios (LR+/LR−) and area under the curve (AUC) were calculated and presented the following results: sensitivity 83.3%, specificity 96.7%, AUC 0.966, LR+ 25.0 and LR− 0.17. Conclusions Results of this pioneering and innovative study demonstrate that anti-T. crassiceps IgY antibodies present potential applicability and can be used as an efficient tool in human NCC serodiagnosis.

Download Full-text