scholarly journals Electrophysiological and Mechanical Characteristics in Human Ileal Motility: Recordings of Slow Waves Conductions and Contractions,In vitro

2015 ◽  
Vol 19 (6) ◽  
pp. 533 ◽  
Author(s):  
Seung-Bum Ryoo ◽  
Heung-Kwon Oh ◽  
Sang Hui Moon ◽  
Eun Kyung Choe ◽  
Sung A Yu ◽  
...  
1984 ◽  
Vol 246 (4) ◽  
pp. G335-G341 ◽  
Author(s):  
A. Bortoff ◽  
L. F. Sillin ◽  
A. Sterns

Spontaneous electrical activity was recorded with bipolar electrodes from the gastrointestinal tracts of unanesthetized fasted cats (upper and lower cut-off frequencies: 35 and 3 Hz). In addition to slow waves (SWs) and spike potentials (SPs), the following three patterns of activity were recorded that are not observed in vitro. 1) Intense bursts of SPs (migrating spike complexes, MSCs) migrate caudally at a velocity of approximately 1 mm/s. MSCs resemble migrating myoelectric complexes (MMCs) in their velocity and by their traversal of intestinal anastomoses. SWs are usually suppressed during and immediately after the MSC, and, on their return, propagate at a higher velocity than they do prior to the MSC. Unlike its effect on MMCs, motilin does not appear to elicit MSCs, a finding consistent with the fact that MSCs occur infrequently in the duodenum and not at all in the antrum. 2) Bursts of SPs are found in the absence of recorded SWs. The SP bursts are of variable duration and occur virtually simultaneously at several recording sites, or propagate at 1-2 cm/s in either direction along the jejunum. The more usual caudally propagating SPs occur when SWs reappear. 3) "Minute rhythms," periods of spiking SWs, occur simultaneously over long lengths of upper bowel, sometimes including antrum, at intervals of about 1-2 min. It is proposed that, despite their differences, the cat MSC may be the functional counterpart of the MMC, that cat SWs are not omnipresent, and that the minute rhythms described here are of central origin.


1999 ◽  
Vol 277 (2) ◽  
pp. G306-G313 ◽  
Author(s):  
Harold G. Preiksaitis ◽  
Nicholas E. Diamant

A myogenic control system (MCS) is a fundamental determinant of peristalsis in the stomach, small bowel, and colon. In the esophagus, attention has focused on neuronal control, the potential for a MCS receiving less attention. The myogenic properties of the cat esophagus were studied in vitro with and without nerves blocked by 1 μM TTX. Muscle contraction was recorded, while electrical activity was monitored by suction electrodes. Spontaneous, nonperistaltic, electrical, and mechanical activity was seen in the longitudinal muscle and persisted after TTX. Spontaneous circular muscle activity was minimal, and peristalsis was not observed without pharmacological activation. Direct electrical stimulation (ES) in the presence of bethanechol or tetraethylammonium chloride (TEA) produced slow-wave oscillations and spike potentials accompanying smooth muscle contraction that progressed along the esophagus. Increased concentrations of either drug in the presence of TTX produced slow waves and spike discharges, accompanied by peristalsis in 5 of 8 TEA- and 2 of 11 bethanechol-stimulated preparations without ES. Depolarization of the muscle by increasing K+ concentration also produced slow waves but no peristalsis. We conclude that the MCS in the esophagus requires specific activation and is manifest by slow-wave oscillations of the membrane potential, which appear to be necessary, but are not sufficient for myogenic peristalsis. In vivo, additional control mechanisms are likely supplied by nerves.


2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Kazimiera H. Bodek ◽  
Karolina M. Nowak ◽  
Marcin Kozakiewicz ◽  
Andrzej Bodek ◽  
Marta Michalska

The aim of this study was to describe the mechanical and sorption features of homogeneous and composite membranes which consist of microcrystalline chitosan (MCCh) and fibrin (Fb) in various proportions as well as thein vitrokinetics of platelet-derived growth factor-BB (PDGF-BB) released from ten types of membranes in the presence or absence of amoxicillin (Am). The films were characterized by Fourier transform infrared (FTIR) spectroscopy, mechanical tests: breaking strength (Bs) and elongation at break (Eb), as well as SEM images, and swelling study. The influence of the form of samples (dry or wet) on Young’s modulus (E) was also examined. The homogeneous MCCh (M1) and composite M3 and M4 (MCCh : Fb = 2 : 1 and 1 : 1) membranes were characterized by good sorption properties and higher mechanical strength, when compared with Fb (M2) membrane. Connecting MCCh with Fb decreases release of PDGF-BB and increases release of Am. The most efficient release of PDGF-BB was observed in the case of M4 (the optimum MCCh : Fb ratio was 1 : 1) membrane. It was found that the degree of PDGF-BB release from the membrane is influenced by the physicochemical and mechanical characteristics of the films and by its affinity to growth factor PDGF-BB.


1970 ◽  
Vol 134 (4) ◽  
pp. 1010-1014 ◽  
Author(s):  
D. B. Young ◽  
H. H. Rostorfer
Keyword(s):  

2016 ◽  
Vol 20 (1) ◽  
Author(s):  
Ramesh Subbiah ◽  
Muhammad Suhaeri ◽  
Mintai Peter Hwang ◽  
Woojun Kim ◽  
Kwideok Park

1986 ◽  
Vol 250 (1) ◽  
pp. G28-G34 ◽  
Author(s):  
N. Suzuki ◽  
C. L. Prosser ◽  
W. DeVos

Electrical slow waves from cat or rabbit small intestine show more variability when recorded in vivo than in vitro. One pattern of variation is waxing and waning of amplitude, or "spindling," during which two rhythms of slightly different frequency come in and out of phase. Fourier power analyses of slow waves during spindles show two frequency peaks of slow waves differing by 0.4-5.0 waves/min and corresponding to measured spindle durations of 12-150 s. Spindles can be induced in vitro in rabbit intestine by K depolarization of approximately 15 mV. Histograms of intracellular recordings of slow nonspindling waves show variation of 0.5-1.0 s on either side of a mean slow wave duration. Spindles are abolished by treatments that reduce electrical coupling between cells, e.g., hypertonic sucrose or lowered pH, but changes in calcium do not alter spindles. Simultaneous recordings by two electrodes in the longitudinal axis show synchrony of spindles at 2- to 3-mm but not at 5-mm separation and synchrony circumferentially to the opposite side of a segment. Contractions, both in vivo and in vitro, correspond with electrical spindles in amplitude. Spindle durations were significantly shorter in vivo than in vitro, indicating a significantly greater difference in vivo in the competing frequencies at the point of recording (P less than 0.01). Three conditions favoring waxing and waning are slight depolarization, variation in slow wave frequency at a point, and electrotonic coupling between muscle fibers. Spindles provide for rhythms of contractions of a 1- to 2-min period.


2018 ◽  
Vol 23 (2) ◽  
pp. 43-51
Author(s):  
Robert Pasławski ◽  
Monika Jacyna ◽  
Krzysztof Jacyna ◽  
Adrian Janiszewski ◽  
Romuald Będziński

Background – In spite of a number of researchers, it is well known that mechanical behaviour of a spinal cord under loading has not yet been studied extensively enough. Methods - Specimens were loaded at various strain rates: 0.02/s and 0.002/s to 5% and 10% strain. After reaching defined strain value, samples were left at a constant strain for stress relaxation. Findings – The demonstrated tensile testing stress-strain response is a highly non-linear curve corresponding to low stiffness. In the toe region stress increases exponentially with the applied strain. The highest calculated stress value for 10% strain was 0,014 MPa (strain rate 0,02/s) and 0,008 MPa (strain rate 0,002/s). Linear approximation of the stress by the least square method allowed to derive Young modulus of the value: 39,68 kPa at strain rate 0,02/s and 31,07 kPa at strain rate 0,002/s. R squared value for both regressions was above 0,99 and confirmed a good quality of approximation. A and β coefficients were 1,5MPa and 31,3 at 0,02/s strain rates and 1,3MPa and 25,3 at 0,002/s strain rates correspondingly. Relative stress relaxation increased from 20% to 37% after 60 s. Absolute stress relaxation was from 0,4kPa to 2,4kPa, at 0,002/s strain rate by 5% maximum strain and 0,02/s strain rate by 10% respectively. Interpretation - Mechanical characteristics demonstrated a visible strain-rate dependence as stiffness was significantly increasing with an increase of strain rate. Mechanical characteristics demonstrated a visible strain-rate dependence as stiffness was significantly increasing with an increase of strain rate.


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