scholarly journals Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi

2015 ◽  
Vol 14 (3) ◽  
pp. 9343-9352 ◽  
Author(s):  
C.F. Sun ◽  
X. Ye ◽  
Y.Y. Tian ◽  
J.J. Dong
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Population Genetic Structure ◽  
Simple Sequence Repeat ◽  
Population Genetic ◽  
Sequence Repeat ◽  
Siniperca Chuatsi ◽  
Genetic Structure Of Populations ◽  
Simple Sequence

scholarly journals Genetic structure of populations of several endangered and endemic Dianthus species revealed by microsatellite markers

2018 ◽  
Vol 77 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Anca Butiuc-Keul ◽  
Cornelia Crăciunaș ◽  
Irina Goia ◽  
Anca Farkas ◽  
Liliana Jarda ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Genetic Structure Of Populations ◽  
Ssr Loci ◽  
Different Populations ◽  
The Mean ◽  
Simple Sequence

Abstract In order to develop a proper conservation programme for several endangered, rare or endemic species of Dianhtus from Romania, molecular characterization by simple sequence repeat (SSR) markers has been accomplished. Amplification of SSR loci in individuals belonging to different populations of D. callizonus, D. glacialis ssp. gelidus, D. henteri, D. nardiformis and D. tenuifolius revealed 23 polymorphic alleles. D. callizonus and D. tenuifolius showed particular sets of SSR alleles. D. glacialis ssp. gelidus, D. henteri and D. nardiformis proved to share almost the same alleles in most of the loci. The highest genetic diversity was observed in D. glacialis ssp. gelidus and D. tenuifolius in locus MS-DINMADSBOX. Allelic patterns across Dianthus species indicate that the mean number of different alleles was highest in D. glacialis ssp. gelidus, while the number of effective alleles was highest in D. tenuifolius. There are no particular differences in individuals belonging to the same species. Genetic diversity is generally low, ranging from 0.18 (D. callizonus) to 0.44 (D. henteri). Regarding the genetic diversity within populations of the same species, no differences were revealed by the use of the SSR markers tested in the present study.

scholarly journals Genetic Diversity of a Natural Population of Akebia trifoliata (Thunb.) Koidz and Extraction of a Core Collection Using Simple Sequence Repeat Markers

2021 ◽  
Vol 12 ◽  
Author(s):  
Yicheng Zhong ◽  
Yue Wang ◽  
Zhimin Sun ◽  
Juan Niu ◽  
Yaliang Shi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Simple Sequence Repeat ◽  
Core Collection ◽  
Polymorphic Information Content ◽  
Germplasm Conservation ◽  
Sequence Repeat ◽  
Information Index ◽  
Molecular Identity ◽  
Simple Sequence

Understand genetic diversity and genetic structure of germplasm is premise of germplasm conservation and utilization. And core collection can reduce the cost and difficulty of germplasm conservation. Akebia trifoliata (Thunb.) Koidz is an important medicinal, fruit and oil crop, particularly in China. In this study, 28 simple sequence repeat (SSR) markers were used to assess the genetic diversity and genetic structure of 955 A. trifoliata germplasms, determine their molecular identity and extract a core collection. The genetic diversity of the 955 germplasms was moderately polymorphic. The average number of alleles (Na), observed heterozygosity (HO), expected heterozygosity (HE), Shannon’s information index (I∗), and polymorphic information content (PIC) were 3.71, 0.24, 0.46, 0.81, and 0.41, respectively. Four subpopulations were identified, indicating a weak genetic structure. A 955 germplasms could be completely distinguished by the characters of s28, s25, s74, s89, s68, s30, s13, s100, s72, s77, and s3. And each germplasm’s molecular identity was made up of eleven characters. The core collection was composed of 164 germplasms (17.2% of 955 total germplasms in the population) and diversity parameters differed significantly from those of a random core collection. These results have implications for germplasm conservation. At the same time, based on the results, the 955 germplasm could be better used and managed.

scholarly journals Genetic Diversity of a Natural Population of Akebia Trifoliata (Thunb.) Koidz and Extraction of a Core Collection Using Simple Sequence Repeat Markers

2020 ◽  
Author(s):  
Yicheng Zhong ◽  
Yue Wang ◽  
Zhimin Sun ◽  
Juan Niu ◽  
Yaliang Shi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Natural Population ◽  
Simple Sequence Repeat ◽  
Core Collection ◽  
Research Report ◽  
Germplasm Management ◽  
Sequence Repeat ◽  
The Core ◽  
Simple Sequence

Abstract Background: Developing a core collection can deepen our understanding of the genetic diversity of germplasm resources and lay the foundation for their rational utilization. Akebia trifoliata (Thunb.) Koidz is an important oil crop, particularly in China, but there is no relevant research report about the core collection of A.trifoliate.Results: In this study, 28 simple sequence repeat (SSR) markers were used to assess the genetic diversity and genetic structure of a natural population of A. trifoliata, including 955 germplasms, and to extract a core collection. The genetic diversity of the natural population was moderately polymorphic. The average number of alleles (Na), observed heterozygosity (HO), expected heterozygosity (HE), Shannon’s information index (I*), and polymorphic information content (PIC) were 3.71, 0.24, 0.46, 0.81, and 0.41, respectively. Two sub-populations were identified, indicating a weak genetic structure. The core collection was composed of 164 individuals (17.2% of 955 total germplasms in the population), and diversity parameters differed significantly from those of a random core germplasm collection. Conclusions: The genetic diversity of the natural population of A.trifoliata was moderately polymorphic, and the genetic structure was weak. Moreover 164 individuals could represent the genetic diversity of the 995 individals. These results have implications for germplasm management and genomics studies in A. trifoliata as well as for the establishment of core collections of other perennial liana species.

Sign in / Sign up

Export Citation Format

Share Document