Allele frequencies of microsatellite loci for genetic characterization of a Sicilian bovine population

We report the development and characterization of 13 novel microsatellite loci for the Caribbean queen conch, Lobatus gigas, an ecologically and commercially important marine gastropod. Paired-end sequencing was carried out on genomic DNA from a single queen conch using half a flow cell lane of an Illumina MiSeq. A total of 48 potentially amplifiable loci containing microsatellites were tested on 45 individuals from the Florida Keys and Bahamas. In total, 13 consistently amplifying and polymorphic microsatellite loci were identified. The number of alleles ranged from 4 to 26 and observed heterozygosities ranged from 0.340 to 1.00. There was no evidence of scoring error, large allele dropout, or evidence of linkage disequilibrium at any locus. Four loci deviated from Hardy-Weinberg equilibrium due to moderate levels of null alleles (null allele frequencies ranged from 0.081 to 0.230). Although null alleles were detected at four microsatellite loci, the high levels of polymorphism and moderate null allele frequencies suggest that these 13 novel microsatellite markers will be useful for researchers carrying out conservation genetic studies of L. gigas.

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Genetic characterization of Albanian grapevine cultivars by microsatellite markers

OENO One ◽

10.20870/oeno-one.2005.39.3.895 ◽

2005 ◽

Vol 39 (3) ◽

pp. 109

Author(s):

Emmanuel D. Ladoukakis ◽

François Lefort ◽

Petraq Sotiri ◽

Arjola Bacu ◽

Efigjeni Kongjika ◽

...

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Genetic Characterization ◽

Allelic Profile ◽

Genetic Profiling ◽

Nuclear Microsatellite ◽

Genetic Profiles

A recently restored ampelographic collection of Albanian grapevine accessions has been submitted to genetic profiling with eleven nuclear microsatellite markers, widely used in other studies. Microsatellite profiling resulted in 28 single profiles for 29 accessions. Two cultivars, Shesh I bardhë and Pucalla, were found to be synonyms. Genetic profiles of Albanian cultivars were compared at 8 microsatellite loci to 29 most commonly cultivated Greek cultivars. Albanian cultivars were found to be more closely related to Greek cultivars from Peloponnese. One Greek cultivar named Dempina was found to be genetically close to two Albanian cultivars Debina teki and Debina kala, which are homonyms Another cultivar, known as Toska or Sinambel displayed a tri-allelic profile at 5 loci over 10 analysed loci. Such a high number of tri-allelic loci found in one individual favours the hypothesis of triploidy but the chimerism hypothesis cannot be excluded without further work.

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Genetic Characterization of Six Stocks ofLitopenaeus vannameiUsed in Cuba for Aquaculture by Means of Microsatellite Loci

International Journal of Zoology ◽

10.1155/2012/352165 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7

Author(s):

Anna Pérez-Beloborodova ◽

Adriana Artiles-Valor ◽

Lourdes Pérez-Jar ◽

Damir Hernández-Martínez ◽

Missael Guerra-Aznay ◽

...

Keyword(s):

Litopenaeus Vannamei ◽

Microsatellite Loci ◽

First Generation ◽

Second Generation ◽

Genetic Characterization ◽

Parental Population ◽

Allelic Variants ◽

The Third ◽

Private Alleles

Four microsatellite loci were used to achieve genetic characterization of six stocks fromLitopenaeus vannameiused for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the crossings from two parental population: first generation from the first with first generation from the third (S1-1 × S1-3) and first generation from the second with first generation from the third (S1-2 × S1-3). 66% (16/24) of genetic systems in total loci were in genetic disequilibrium. The four microsatellite loci were polymorphic for all six stocks. Major quantities of allelic variants correspond to locus Pvan 1758, which is at the same time that one where there are private alleles from first generation of the third. All Fst comparisons were significant. This indicates big differences between stocks. The highest values are those in which there is presence of the second introduction. This introduction and its descendants are also more consanguineous.

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Genetic characterization of Akhal-Teke horse subpopulations using 17 microsatellite loci

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/341/1/012070 ◽

2019 ◽

Vol 341 ◽

pp. 012070 ◽

Cited By ~ 1

Author(s):

A V Ustyantseva ◽

L A Khrabrova ◽

N V Abramova ◽

T N Ryabova

Keyword(s):

Microsatellite Loci ◽

Genetic Characterization

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Application of Novel Polymorphic Microsatellite Loci Identified in the Korean Pacific Abalone (Haliotis diversicolor supertexta (Haliotidae)) in the Genetic Characterization of Wild and Released Populations

International Journal of Molecular Sciences ◽

10.3390/ijms130910750 ◽

2012 ◽

Vol 13 (9) ◽

pp. 10750-10764 ◽

Cited By ~ 8

Author(s):

Hye Suck An ◽

Jang Wook Lee ◽

Seong Wan Hong

Keyword(s):

Microsatellite Loci ◽

Genetic Characterization ◽

Haliotis Diversicolor ◽

Pacific Abalone ◽

Haliotis Diversicolor Supertexta ◽

Polymorphic Microsatellite ◽

Diversicolor Supertexta

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Microsatellite marker analysis of macedonian grapevines (Vitis vinifera L.) compared to Bulgarian and Greek cultivars

OENO One ◽

10.20870/oeno-one.2009.43.1.805 ◽

2009 ◽

Vol 43 (1) ◽

pp. 29

Author(s):

Natasa Stajner ◽

Elizabeta Angelova ◽

Zvonimir Bozinovic ◽

Mihail Petkov ◽

Branka Javornik

Keyword(s):

Vitis Vinifera ◽

Microsatellite Marker ◽

Microsatellite Loci ◽

Genetic Background ◽

Genetic Characterization ◽

Molecular Investigation ◽

Balkan Region ◽

Shared Alleles ◽

Microsatellite Marker Analysis

Aims: Eleven Macedonian grapevine accessions were genotyped by microsatellite profiling at 9 microsatellite loci, in order to identify Macedonian cultivars and to evaluate the relationships among them. The comparison with grapevine cultivars from two neighbouring countries was also performed.Methods and results: Clustering analyses based on the proportion of shared alleles resulted in two clusters containing all accessions except cultivar « Vranec », which was distant from the others. Comparison of genotyping results of Macedonian accessions with 76 Bulgarian and 298 Greek accessions revealed no identical genotypes. In the dendogram, Macedonian accessions are dispersed among Greek and Bulgarian grapevines, suggesting a common genetic background. Additionaly, the synonyms « Smederevka » = « Dimyat » = « Zoumiatiko » and « Belo Zimsko » = « Karatsova Naousis » were also evaluated.Conclusions: Clustering analyses showed that authentic Macedonian cultivars are distant from two widespread cultivars « Vranec » and « Smederevka ». Comparison of Macedonian cultivars with their synonyms from Greece and Bulgaria revealed differences in allelic profiles at some loci, but further analyses are needed to confirm their unique allelic profiles.Significance and impact of study: This work is a first step towards the genetic characterization of Macedonian grapevine germplasm, thus contributing to the molecular investigation of grapevine germplasm within the Balkan region.

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