scholarly journals Experimental Evidence on the Nature of the Antigen in the Direct Agglutination Test for Visceral Leishmaniasis

2020 ◽  
Vol 102 (4) ◽  
pp. 788-796
Author(s):  
Vera Kühne ◽  
Ruben Verstraete ◽  
Xaveer van Ostade ◽  
Philippe Büscher
Keyword(s):  
Visceral Leishmaniasis ◽  
Experimental Evidence ◽  
Agglutination Test ◽  
Direct Agglutination Test

scholarly journals DIRECT AGGLUTINATION TEST WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS

2004 ◽  
Vol 70 (1) ◽  
pp. 78-82 ◽  
Author(s):  
MOHAMMAD ZAHIDUL ISLAM ◽  
IFTIKHAR AHMED ◽  
ABDUL HALIM SARDER ◽  
RUSELLA MIRZA ◽  
EISAKU KIMURA ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Agglutination Test ◽  
Urine Samples ◽  
Direct Agglutination Test

scholarly journals A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis

2016 ◽  
Vol 221 ◽  
pp. 9-13 ◽  
Author(s):  
Edward Oliveira ◽  
Juliana Wilke Saliba ◽  
Diana Oliveira ◽  
Edelberto Santos Dias ◽  
Gustavo Fontes Paz
Keyword(s):  
Visceral Leishmaniasis ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test ◽  
Test Kit

Serodiagnosis and Screening of Canine Visceral Leishmaniasis in an Endemic Area of Corsica: Applicability of a Direct Agglutination Test and Immunoblot Analysis

1992 ◽  
Vol 47 (6) ◽  
pp. 772-777 ◽  
Author(s):  
Asit B. Neogy ◽  
Didier Rzepka ◽  
Yiannis Tselentis ◽  
Jean C. Lascombe ◽  
Ioannis Vouldoukis ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Endemic Area ◽  
Immunoblot Analysis ◽  
Agglutination Test ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test

Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis

2011 ◽  
Vol 105 (7) ◽  
pp. 414-416 ◽  
Author(s):  
Edward Oliveira ◽  
Soraya Wilke Saliba ◽  
Camila Filizzola de Andrade ◽  
Ana Rabello
Keyword(s):  
Visceral Leishmaniasis ◽  
Laboratory Diagnosis ◽  
Agglutination Test ◽  
Direct Agglutination Test

scholarly journals Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis

2014 ◽  
Vol 63 (1) ◽  
pp. 106-110
Author(s):  
Saul José Semião-Santos ◽  
Laura Barral Veloso ◽  
Paulo Paes de Andrade ◽  
Marcia Almeida de Melo ◽  
Luis Miguel Lourenço Martins ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Leishmania Donovani ◽  
Agglutination Test ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test ◽  
Serum Samples

We compared the performance of a locally produced β-mercaptoethanol-modified promastigote antigen (β-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for β-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for β-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s κ = 0.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of β-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.

scholarly journals Dog-DAT: a direct agglutination test using stabilized, freeze-dried antigen for the serodiagnosis of canine visceral leishmaniasis

1996 ◽  
Vol 16 (3-4) ◽  
pp. 235-239 ◽  
Author(s):  
Linda Oskam ◽  
Rob J. Slappendel ◽  
Eef G.M. Beijer ◽  
Nel C.M. Kroon ◽  
Cor W. van Ingen ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Agglutination Test ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test ◽  
Freeze Dried

Evaluation of direct agglutination test (dat) and elisa for serodiagnosis of visceral leishmaniasis in india

1991 ◽  
Vol 5 (5) ◽  
pp. 303-306 ◽  
Author(s):  
Abhijit Pal ◽  
Krishna Mukerji ◽  
Kshudiram Naskar ◽  
Dilip K. Ghosh ◽  
Debashish Basu ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Agglutination Test ◽  
Direct Agglutination Test

scholarly journals Use of a Newly Developed β-Mercaptoethanol Enzyme-Linked Immunosorbent Assay To Diagnose Visceral Leishmaniasis in Patients in Eastern Sudan

2007 ◽  
Vol 14 (12) ◽  
pp. 1592-1595 ◽  
Author(s):  
Durria Mansour ◽  
Elfadil M. Abass ◽  
Mohamed el Mutasim ◽  
Abdelhafeiz Mahamoud ◽  
Abdallah el Harith
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Leishmania Donovani ◽  
Clinical Evidence ◽  
High Reliability ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Symptomatic Group ◽  
Eastern Sudan

ABSTRACT Corroboration of serology results is essential for restricting the risk of inappropriate antileishmanial prescription. A direct agglutination test (DAT) and a recently developed β-mercaptoethanol-modified enzyme-linked immunosorbent assay (β-ME ELISA) based on the use of antigen prepared as described for the DAT were applied to 416 sera from two Sudanese populations with and without clinical evidence of visceral leishmaniasis (VL). Of 285 sera with the lowest antileishmanial DAT titers (≤1:100 to 1:1,600), 270 (94.7%) scored comparable minimum β-ME ELISA absorbance values (≤0.1 to 0.26). In 117 sera that demonstrated the highest DAT titers (1:12,800 to ≥1:25,600), 86 (73.5%) scored maximum (0.81 to ≥1.35) and 30 (25.6%) medium (0.27 to 0.80) β-ME ELISA absorbance values. VL diagnosis was established for 142 (44.1%) patients in the VL-symptomatic group (n = 322), based on positive microscopy for Leishmania donovani in lymph node aspirates or positive DAT (titer, ≥1:3,200). Of the 125 sera from the symptomatic patients for whom microscopy was positive for VL, 111 (88.8%) had comparable positive DAT and β-ME ELISA readings. In all 17 sera from the symptomatic DAT-positive patients for whom leishmaniasis was not established by microscopy but who responded favorably to antileishmanial therapy, absorbance values (≥0.27) indicative of VL were obtained by β-ME ELISA. Of 197 symptomatic patients for whom microscopy was negative for VL, 172 (87.3%) tested negative in β-ME ELISA and 180 (91.4%) in DAT. Based on the high reliability demonstrated here for VL detection, β-ME ELISA fulfills the requirement of confirming DAT results in patients manifesting suspected VL.

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