scholarly journals Evaluation of Two rK39 Dipstick Tests, Direct Agglutination Test, and Indirect Fluorescent Antibody Test for Diagnosis of Visceral Leishmaniasis in a New Epidemic Site in Highland Ethiopia

2011 ◽  
Vol 84 (1) ◽  
pp. 102-106 ◽  
Author(s):  
Carmen Cañavate ◽  
Caryn Bern ◽  
Carmen Chicharro ◽  
Anna J. Blackstock ◽  
Jorge Alvar ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody

scholarly journals Comparison of Enzyme-Linked Immunosorbent Assay, Indirect Fluorescent Antibody Test, and Direct Agglutination Test for Detecting Toxoplasma Gondii Antibodies in Naturally Aborted Ovine Fetuses

1989 ◽  
Vol 1 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Sheryl L. Seefeldt ◽  
Clyde A. Kirkbride ◽  
Jitender P. Dubey
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Special Equipment

Results obtained in an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFA), and a modified direct agglutination test (MAT) for Toxoplasma gondii antibodies from examination of fetal fluids from 377 aborted ovine fetuses were compared. Sixty-seven samples were positive by MAT (titers 1:16 to > 1:65,536), 58 were positive by ELISA, and 62 were positive by immunoglobulin G-IFA. The MAT was preferred because it required less time, labor, and special equipment. It was simple to run, could be done on serum from any species without modification, and it was more effective than the IFA for detecting toxoplasma antibodies in severely autolyzed fetuses. No advantage was found in determining immunoglobulin M antibodies in ovine fetal sera.

scholarly journals A Modified Agglutination Test for Neospora caninum: Development, Optimization, and Comparison to the Indirect Fluorescent-Antibody Test and Enzyme-Linked Immunosorbent Assay

1998 ◽  
Vol 5 (4) ◽  
pp. 467-473 ◽  
Author(s):  
Andrea E. Packham ◽  
Karen W. Sverlow ◽  
Patricia A. Conrad ◽  
Emily F. Loomis ◽  
Joan D. Rowe ◽  
...  
Keyword(s):  
Indirect Fluorescent Antibody Test ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Secondary Antibodies ◽  
Higher Sensitivity

ABSTRACT Current serologic tests used to detect antibodies to Neospora caninum require species-specific secondary antibodies, limiting the number of species that can be tested. In order to examine a wide variety of animal species that may be infected with N. caninum, a modified direct agglutination test (N-MAT) similar to the Toxoplasma gondii modified direct agglutination test (T-MAT) was developed. This test measures the direct agglutination of parasites by N. caninum-specific antibodies in serum, thus eliminating the need for secondary host-specific anti-isotype sera. The N-MAT was compared to the indirect fluorescent-antibody test (IFAT) and the enzyme-linked immunosorbent assay (ELISA) with a “gold standard” serum panel from species for which secondary antibodies were available (n = 547). All positive samples tested were from animals with histologically confirmed infections. Up to 16 different species were tested. The N-MAT gave a higher sensitivity (100%) and specificity (97%) than the ELISA (74 and 94%, respectively) and had a higher sensitivity but a lower specificity than the IFAT (98 and 99%, respectively). The reduced specificity of the N-MAT was due to false-positive reactions in testing fetal fluids with particulate matter or severely hemolyzed serum. Overall, the N-MAT proved to be highly sensitive and specific for both naturally and experimentally infected animals, highly reproducible between and within readers, easy to use on large sample sizes without requiring special equipment, and useful in testing serum from any species without modification.

scholarly journals Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil

2016 ◽  
Vol 25 (1) ◽  
pp. 109-111 ◽  
Author(s):  
Solange Maria Gennari ◽  
Hilda Fátima de Jesus Pena ◽  
David Scott Lindsay ◽  
Marcos Gomes Lopes ◽  
Herbert Sousa Soares ◽  
...  
Keyword(s):  
Animal Species ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Northeastern Brazil ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Sarcocystis Neurona ◽  
Neurological Illness

Abstract Sarcocystis neurona and Neospora hughesi are coccidian protozoa that can cause neurological illness in horses in America. In this study we report seroprevalence of Neospora spp. andS. neurona in sera of 333 donkeys from the northeastern region of Brazil. Antibodies to Neospora spp. were detected in 2% (7 donkeys) of 333 sera tested by the indirect fluorescent antibody test (IFAT) with a cut-off dilution of 1:40. Antibodies to S. neurona were found in 3% (10 donkeys) of the samples tested by IFAT (cut-off ≥50) and 21% (69 donkeys) by the direct agglutination test (SAT ≥50). The SAT and IFAT results for S. neurona showed a poor concordance (value of Kappa=0.051). This is the first report ofNeospora spp. antibodies in Brazilian donkeys and the first detection of antibodies against S. neurona in this animal species.

Lysogeny in the mycobacteria. II. Alterations of bacterial antigens mediated by mycobacteriophage

1969 ◽  
Vol 15 (9) ◽  
pp. 1112-1114 ◽  
Author(s):  
Wilbur D. Jones Jr. ◽  
R. Edward Beam
Keyword(s):  
Mycobacterium Smegmatis ◽  
Bacterial Culture ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Bacterial Antigen ◽  
Indirect Fluorescent Antibody ◽  
Bacterial Antigens ◽  
Mycobacterium Smegmatis Atcc 607

Using the agglutination test and the indirect fluorescent antibody test it was demonstrated that lysogenization of the rough variant of Mycobacterium smegmatis ATCC 607 by mycobacteriophages D29 and B4 was accompanied by the appearance of new bacterial antigens. Each phage mediated a different bacterial antigen. That the new bacterial antigens were phage mediated was demonstrated by the loss of the new antigens when the bacterial culture was "cured" of the prophage.

Comparison of indirect fluorescent antibody test and the modified agglutination test for the detection of Toxoplasma gondii antibodies in stray dogs from Southern Brazil

2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Jonatas Campos de Almeida ◽  
Michelle Salmon Frehse ◽  
Italmar Teodorico Navarro ◽  
João Luis Garcia ◽  
Alexander Welker Biondo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Southern Brazil ◽  
Stray Dogs ◽  
Modified Agglutination Test

AbstractThe aim of the present study was to determine the prevalence of antibodies to

scholarly journals Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs

2004 ◽  
Vol 24 (4) ◽  
pp. 199-202 ◽  
Author(s):  
Alessandro Pelegrine Minho ◽  
Roberta Lemos Freire ◽  
Odilon Vidotto ◽  
Solange Maria Gennari ◽  
Elizabete Marangoni Marana ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Confidence Interval ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Antibody Detection ◽  
Indirect Fluorescent Antibody ◽  
Serum Samples ◽  
Modified Agglutination Test

The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.

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