Serodiagnosis and Screening of Canine Visceral Leishmaniasis in an Endemic Area of Corsica: Applicability of a Direct Agglutination Test and Immunoblot Analysis

We compared the performance of a locally produced β-mercaptoethanol-modified promastigote antigen (β-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for β-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for β-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s κ = 0.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of β-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.

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Dog-DAT: a direct agglutination test using stabilized, freeze-dried antigen for the serodiagnosis of canine visceral leishmaniasis

FEMS Immunology & Medical Microbiology ◽

10.1111/j.1574-695x.1996.tb00141.x ◽

1996 ◽

Vol 16 (3-4) ◽

pp. 235-239 ◽

Cited By ~ 17

Author(s):

Linda Oskam ◽

Rob J. Slappendel ◽

Eef G.M. Beijer ◽

Nel C.M. Kroon ◽

Cor W. van Ingen ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Agglutination Test ◽

Canine Visceral Leishmaniasis ◽

Direct Agglutination Test ◽

Freeze Dried

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THE USE OF IMMUNOBLOT ANALYSIS IN THE DIAGNOSIS OF CANINE VISCERAL LEISHMANIASIS IN AN ENDEMIC AREA OF RIO DE JANEIRO

Journal of Parasitology ◽

10.1645/ge-2988 ◽

2003 ◽

Vol 89 (4) ◽

pp. 832-836 ◽

Cited By ~ 13

Author(s):

Adelzon A. de Paula ◽

Alba M. V. da Silva ◽

Octávio Fernandes ◽

Ana M. Jansen

Keyword(s):

Visceral Leishmaniasis ◽

Rio De Janeiro ◽

Endemic Area ◽

Immunoblot Analysis ◽

Canine Visceral Leishmaniasis

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Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health?

Ciência Rural ◽

10.1590/0103-8478cr20160846 ◽

2017 ◽

Vol 47 (4) ◽

Cited By ~ 2

Author(s):

Monique Paiva de Campos ◽

Paula Mello De Luca ◽

Alinne Rangel dos Santos Renzetti ◽

Sara Maria Marques de Souza ◽

Artur Augusto Velho Mendes Júnior ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Enzyme Immunoassay ◽

Clinical Evaluation ◽

Laboratory Tests ◽

Endemic Area ◽

Canine Visceral Leishmaniasis ◽

Ministry Of Health ◽

Evaluation Time ◽

One Year ◽

Serological Diagnostics

ABSTRACT: The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations of this dog were negative. Our results showed that in a non-endemic area, even at different evaluation times, dogs vaccinated against CVL with Leishmune® or Leish tec® did not seroconvert in the serological protocol used by the Brazilian Ministry of Health (DPP/EIE).

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